Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls

Cookson, Sarah Jane; Clemente‐Moreno, María José; Hévin, Cyril; Mendome, L. Z. Nyamba; Delrot, Serge; Magnin, Noël; Trossat-Magnin, Claudine; Ollat, Nathalie

doi:10.1093/jxb/eru145

Cited by 97 publications

(91 citation statements)

References 42 publications

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“…Some degree of immune response to grafting is experienced even in these compatible genotypes, and a recent report demonstrated the up-regulation of stress responses in heterografting with nonself rootstocks (Cookson et al, 2014). This general response also could be the basis of the changes in expression that we observed for some candidate miRNAs.…”

Section: The Accumulation Of Some Novel Mirnas Is Differently Modulatsupporting

confidence: 57%

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

et al. 2017

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Grapevine (Vitis vinifera) is routinely grafted, and rootstocks inducing drought tolerance represent a source for adapting vineyards to climate change in temperate areas. Our goal was to investigate drought stress effects on microRNA (miRNA) abundance in a drought-resistant grapevine rootstock, M4 (Vitis vinifera 3 Vitis berlandieri), compared with a commercial cultivar, Cabernet Sauvignon, using their autografts and reciprocal grafts. RNA extracted from roots and leaves of droughted and irrigated plants of different graft combinations was used to prepare cDNA libraries for small RNA sequencing and to analyze miRNAs by quantitative real-time polymerase chain reaction (RT-qPCR). Measurements of leaf water potential, leaf gas exchange, and root hydraulic conductance attested that, under irrigation, M4 reduced water loss in comparison with cultivar Cabernet Sauvignon mostly through nonhydraulic, root-specific mechanisms. Under drought, stomatal conductance decreased at similar levels in the two genotypes. Small RNA sequencing allowed the identification of 70 conserved miRNAs and the prediction of 28 novel miRNAs. Different accumulation trends of miRNAs, observed upon drought and in different genotypes and organs, were confirmed by RT-qPCR. Corresponding target transcripts, predicted in silico and validated by RT-qPCR, often showed opposite expression profiles than the related miRNAs. Drought effects on miRNA abundance differed between the two genotypes. Furthermore, the concentration of drought-responsive miRNAs in each genotype was affected by reciprocal grafting, suggesting either the movement of signals inducing miRNA expression in the graft partner or, possibly, miRNA transport between scion and rootstock. These results open new perspectives in the selection of rootstocks for improving grapevine adaptation to drought.

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Section: The Accumulation Of Some Novel Mirnas Is Differently Modulatsupporting

confidence: 57%

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

et al. 2017

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“…Although only limited numbers of papers have been reported for scion gene expression change by grafting, it was revealed that heterografts induce more stress-responsive genes than autografts (Cookson et al 2014). Our results are different from previous reports since rootstocks and scions from two related vegetable species were used.…”

Section: Identification Of Genes Showing Rootstock-dependent Responsescontrasting

confidence: 99%

Grafting-Induced Gene Expression Change in Brassica rapa Leaves is Different from Fruit Trees

Mun¹,

Yi²,

Nou³

et al. 2015

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2015 (March) ABSTRACT Grafting has widely used in the agriculture of fruit-bearing crops and trees because rootstocks have a profound influence on many aspects of scion development and scion responses to the environmental changes including biotic and abiotic stress tolerance. These effects appear to result from the change of gene expression on scion, but only limited numbers of papers have been published demonstrating it. To identify altered expression of genes in Chinese cabbage, Chiifu(Brassica rapa ssp. pekinensis, inbred line) shoot was grafted on three Brassica rootstocks: mustard, turnip and broccoli. After head formation, Br300K microarray experiment was conducted using total RNAs from scion leaves collected in two different seasons, spring (June) and fall (October). A large number of differentially expressed genes (DEGs) were identified both in two seasonal samples, but DEGs were more notable in June sample than in October sample. However, the number of DEGs by three rootstocks were high in October with respect to up-regulation, but high in June for down-regulation. Categories of DEGs included metal ion binding, response to hormonal stimuli, response to endogenous stimuli, regulation of transcription, oxidation reduction and response to stress. Up-regulated genes in both June and October samples were similar in mustard and turnip rootstocks, but different in broccoli rootstock. Two genes were found to respond to all experimental conditions: Brapa_ESTC049008 (hypothetical protein) as an up-regulated gene and Brapa_ESTC016027 (CNGC12) as a down-regulated gene. Together with the previous reports, these results suggest that grafting-induced gene expression depends on the species involved.

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“…5 We have shown that superimposed upon the gene expression differences occurring in homografts, additional stress and/or defense related genes are differentially expressed. 5 These gene expression changes were not accompanied by alteration in the histology of the graft interface in terms of callose, cellulose and lignin accumulation (data not shown).…”

Section: Hetero-grafting Triggers the Up-regulation Of Stress And/or mentioning

confidence: 92%

“…3 Despite the widespread use of grafting, the sequence of the complex biochemical and structural process that lead to successfully graft union formation remains largely uncharacterized. We have been studying the gene expression differences involved in graft union formation in grapevine homo-grafts (cabernet sauvignon (CS) grafted with CS 4 ) and hetero-grafts (CS grafted with rootstocks 5 ).…”

mentioning

confidence: 99%

Developments at the graft interface in homo- and hetero-grafts

Clemente‐Moreno

Hévin

Ollat

et al. 2014

Plant Signaling & Behavior

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Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls

Abstract: SummaryGrafting together two different genotypes results in the upregulation of stress responses at the graft interface during graft union formation in comparison to the wound-like responses of autografts.

Cited by 97 publications

References 42 publications

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

Grafting-Induced Gene Expression Change in Brassica rapa Leaves is Different from Fruit Trees

Developments at the graft interface in homo- and hetero-grafts

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