Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

Sugie, Atsushi; Murai, Koji; Takumi, Shigeo

doi:10.1266/ggs.82.231

Cited by 25 publications

(27 citation statements)

References 35 publications

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“…Distribution of the Ne1 and Ne2 dominant alleles is postulated to be due to selection advantages such as rust fungus resistance , although no direct evidence proving a relationship between type I necrosis and disease resistance has been reported. In leaves of wheat showing type I necrosis, superoxide is highly accumulated and antioxidant enzymes show high levels of activity (Dalal and Khanna-Chopra 2001;KhannaChopra et al 1998;Sugie et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

et al. 2015

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Hybrid necrosis is a well-known reproductive isolation mechanism in plant species, and an autoimmune response is generally considered to trigger hybrid necrosis through epistatic interaction between disease resistance-related genes in hybrids. In common wheat, the complementary Ne1 and Ne2 genes control hybrid necrosis, defined as type I necrosis. Two other types of hybrid necrosis (type II and type III) have been observed in interspecific hybrids between tetraploid wheat and Aegilops tauschii. Another type of hybrid necrosis, defined here as type IV necrosis, has been reported in F1 hybrids between Triticum urartu and some accessions of Triticum monococcum ssp. aegilopoides. In types I, III and IV, cell death occurs gradually starting in older tissues, whereas type II necrosis symptoms occur only under low temperature. To compare comprehensive gene expression patterns of hybrids showing growth abnormalities, transcriptome analysis of type I and type IV necrosis was performed using a wheat 38k oligo-DNA microarray. Defense-related genes including many WRKY transcription factor genes were dramatically up-regulated in plants showing type I and type IV necrosis, similarly to other known hybrid abnormalities, suggesting an association with an autoimmune response. Reactive oxygen species generation and necrotic cell death were effectively inhibited by ZnCl2 treatment in types I, III and IV necrosis, suggesting a significant association of Ca(2+) influx in upstream signaling of necrotic cell death in wheat hybrid necrosis.

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Section: Introductionmentioning

confidence: 99%

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

et al. 2015

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“…Total RNA was extracted from the seedling leaves and treated with DNaseI to remove the contaminated DNA. Reverse transcriptase (RT)-PCR with the gene-specific primer sets resulted in amplification of the specific single fragments as described previously (Sugie et al, 2006(Sugie et al, , 2007. RT-PCR for wheat uncoupling protein 1 (WhUCP1) was conducted with the following primers: 5 0 -TGAAAGTGAGATTGCAAGCA-3 0 and 5 0 -GCCCAT-CATTCTTGATTTCA-3 0 .…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial alternative pathway is associated with development of freezing tolerance in common wheat

Mizuno

Sugie

Kobayashi

et al. 2008

Journal of Plant Physiology

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Cold acclimation is an adaptive process for acquiring cold/freezing tolerance in wheat. To clarify the cultivar difference of freezing tolerance, we compared mitochondrial respiration activity and the expression profile of alternative oxidase (AOX) genes under low-temperature conditions using two common wheat cultivars differing in freezing tolerance. During cold acclimation, the respiration capacity of the alternative pathway significantly increased in a freezing-tolerant cultivar compared with a freezing-sensitive cultivar. More abundant accumulation of the AOX and uncoupling protein gene transcripts was also observed under the low-temperature conditions in the tolerant cultivar than in the sensitive cultivar. These results suggest that the mitochondrial alternative pathway might be partly associated with the cold acclimation and freezing tolerance in wheat.

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“…Necrotic cell death induced by the Ne1-Ne2 epistatic interaction is accompanied with generation of reactive oxygen (Sugie et al 2007 ). Because the Ne2 gene seems to be located in a chromosomal region closely linked to the R gene against rust fungus, it has been postulated that type I necrosis is due to autoimmune-like responses triggered by the Ne1 -Ne2 interaction (Bomblies and Weigel 2007 ).…”

Section: Abnormal Phenotypes In Wheat Hybridsmentioning

confidence: 99%

Association of Wheat miRNAs with Hybrid Incompatibility in Interspecific Crosses of Triticum and Aegilops

Takumi

Matsuda

Iehisa

2015

Advances in Wheat Genetics: From Genome to Field

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Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

Cited by 25 publications

References 35 publications

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

Mitochondrial alternative pathway is associated with development of freezing tolerance in common wheat

Association of Wheat miRNAs with Hybrid Incompatibility in Interspecific Crosses of Triticum and Aegilops

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