A genetic basis for resistance to enteric disease caused by E. coli

Rutter, J. M.; Burrows, M. R.; Sellwood, R.; Gibbons, R. A.

doi:10.1038/257135a0

Cited by 129 publications

(46 citation statements)

References 13 publications

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“…One of the factors that has an influence on the clinical signs is the presence of an adhesion site in the small intestine, which is usually referred to as the F4 receptor (F4R) or K88 receptor [4][5][6]. This adhesion site is a genetically inherited dominant characteristic and its presence can be shown by in vitro adhesion assays [7,8].…”

Section: Introductionmentioning

confidence: 99%

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Geenen

Meulen²,

Bouma

et al. 2004

Epidemiol. Infect.

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SUMMARY F4+Escherichia coli is an important agent of post-weaning diarrhoea in piglets. Piglets that express an adhesion site for F4+ E. coli in their small intestine (F4R+) shed higher numbers of F4+ E. coli than piglets lacking this site (F4Rx). We hypothesized that F4R+ piglets are more infectious and more susceptible for F4+ E. coli. This implies that in populations with F4R+ and F4Rx piglets, the transmission would be dependent on the frequency of both types of animals. To quantify the difference in infectiousness and susceptibility, a one-to-one transmission experiment was performed with 20 pairs consisting of one inoculated and one contact piglet. Based on the contact infections observed, transmission parameters were estimated with generalized linear models. F4R+ piglets were infectious for other piglets and the reproduction ratio (R 0 ) for homogeneous F4R+ populations, that is the average number of secondary infections that one F4R+ pig will cause during its entire infectious period in a population of susceptible F4R+ individuals only, was estimated as 7 . 1. F4R+ piglets were more susceptible than F4Rx piglets and reducing the fraction of F4R+ piglets of a population will reduce transmission. It was calculated that in order to prevent major outbreaks of F4+ E. coli (R 0 <1), the fraction of F4R+ piglets must be lower than 0 . 14.

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Section: Introductionmentioning

confidence: 99%

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Geenen

Meulen²,

Bouma

et al. 2004

Epidemiol. Infect.

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“…The receptor for K88 antigen, defining the susceptibility of the piglet to neonatal E. coli diarrhea, is expressed by a dominant allele (13). Piglets lacking the receptor (homozygous recessive) are resistant to infection.…”

Section: Introductionmentioning

confidence: 99%

Oral immunization of sows: Anti‐K88 antibodies in serum and milk of the sow and in serum of the piglets

Kortbeek-Jacobs¹,

Houten²

1982

Veterinary Quarterly

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“…3), F4R seemed to be the major factor affecting the shedding profiles. This can be explained by the differences of specific adhesion to F4R on the brush borders of the pig [10] which is important with respect to colonization of F4+ E. coli [5,7]. In ref.…”

Section: Discussionmentioning

confidence: 99%

“…F4R promotes adherence of F4+ E. coli to the brush border of small-intestinal enterocytes and is important for the colonization and replication of F4+ E. coli in the small intestine, which is followed by production of enterotoxins (resulting in diarrhoea) [5]. In earlier studies, diarrhoea was found to be more prevalent in F4R+ pigs [7,9,11]. Pigs can be classified upon slaughter by in vitro adhesion assay [10] into F4R+ (adherent brush borders) and F4Rx (nonadherent brush borders).…”

Section: Introductionmentioning

confidence: 99%

“…However, before we were able to study transmission and diarrhoea among pigs, we needed to find a measure to discriminate between infectious and noninfectious pigs and a measure to discriminate between diarrhoeic and non-diarrhoeic pigs. Discrimination is far from straightforward since the colonization and replication of F4+ E. coli within the intestine [5] and clinical symptoms vary considerably [6][7][8][9]. The number of F4+ E. coli shed in the faeces as a function of time might be one of the measures of infectiousness of the pigs, which is of importance with respect to transmission.…”

Section: Introductionmentioning

confidence: 99%

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Classification of temporal profiles of F4+E. colishedding and faecal dry matter in experimental post-weaning diarrhoea of pigs

Geenen¹,

Meulen²,

Bouma

et al. 2006

Epidemiol. Infect.

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SUMMARYEnterotoxigenic F4+ Escherichia coli can colonize the intestine of pigs and cause diarrhoea. Our primary goal was to find a discriminant rule to discriminate between F4+ E. coli shedding profiles as this may reflect differences in the infectiousness of pigs. Our secondary goal was to find a discriminant rule to discriminate between diarrhoeic and non-diarrhoeic pigs. Repeated measurements (bacterial shedding and percentage dry matter of faeces) were taken of 74 weaned pigs that were infected experimentally with F4+ E. coli. These measurements were summarized into two new variables by means of a principal components analysis. Discriminant rules were derived based on these summary variables by fitting a mixture of normal distributions. Finally, the association between the classifications (as derived from the discriminant rules) and the occurrence in the pigs of the F4 receptor, an adhesion site for F4+ E. coli, was studied. We found that only the classification based on bacterial shedding allowed us to distinguish two significantly different groups of pigs (high and low shedders). Presence of the F4 receptor was associated strongly with pigs being high shedders.

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A genetic basis for resistance to enteric disease caused by E. coli

Cited by 129 publications

References 13 publications

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Oral immunization of sows: Anti‐K88 antibodies in serum and milk of the sow and in serum of the piglets

Classification of temporal profiles of F4+E. colishedding and faecal dry matter in experimental post-weaning diarrhoea of pigs

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