A GC/MS method for the simultaneous determination and quantification of chlorpromazine and diazepam in pork samples

Abstract

A gas chromatography coupled with mass spectrometry (GC/MS) method for the simultaneous determination of chlorpromazine and diazepam in pork samples, which are two of the most often used antipsychotics, has been developed and validated. The samples were prepared and purified by solidphase extraction (SPE), and then were analyzed by GC/MS. Chromatography was performed using an HP-5 MS 5% phenyl-95% methylpolysiloxane fused silica capillary column (30 m  0.25 mm; film thickness 0.25 mm). The method had a linear calibration curve over the range 10-500 ng mL À1 (r > 0.99), recovery (>66.5% for chlorpromazine and >77.4% for diazepam), precision (%RSD < 11.5% for chlorpromazine and <9.5% for diazepam). The limit of quantification for both chlorpromazine and diazepam was 2.0 mg kg À1 . This method can result in faster analysis, lower solvent consumption and less workload per sample while maintaining or even improving sensitivity.

Electroanalytical determination of diazepam in tablet and human serum samples using a multiwalled carbon nanotube embedded molecularly imprinted polymer-modified carbon paste electrode

“…It is also an abused drug in which sudden withdrawal, particularly from high doses, carries the risk of epileptic seizures. 2 A variety of analytical methods have been developed for the determination of this drug in both pharmaceutical and biological samples, such as spectrophotometry, [3][4][5][6] different chromatographic methods (HPLC, LC/MS, GC, GC/MS, and TLC), [7][8][9][10][11] dispersive liquid-liquid microextraction, 12 capillary electrophoresis, 2 radioimmunoassay 13 and electrochemical methods. [14][15][16][17][18][19] Among these methods, electrochemical techniques are a powerful and versatile analytical technique that offers high sensitivity, accuracy, and precision as well as a large linear dynamic range, with relatively low-cost instrumentation than other methods.…”

Extraction and determination of trace amounts of chlorpromazine in biological fluids using magnetic solid phase extraction followed by HPLC

“…However, in many cases, owing to matrix interference and insufficient instrumental detection limit for (ultra) trace level of analyte in real biological samples, direct chromatographic separation and determination of the species is difficult [7] . Therefore, in order to obtain accurate, reliable and sensitive results, a separation/preconcentration method is required prior to chromatographic separation and/or other determination techniques [7] , [8] . Consequently, separation/preconcentration techniques such as solid phase microextraction [9] , hollow fiber liquid phase microextraction (HF-LPME) [10] and molecularly imprinted polymer [11] have been reported for extraction of CPZ from biological fluids.…”

New Fluorescent Nanosensor for Determination of Diazepam Using Molecularly Imprinted Mn-doped ZnS Quantum Dots

“…Overdose symptoms include drowsiness, confusion, coma, and decreased reflexes (2,3). Various analytical methods have been used to determine diazepam in both biological and pharmaceutical samples, such as spectrophotometry (4,5), capillary electrophoresis (2), electrochemical methods (6)(7)(8), and various chromatographic methods (9)(10)(11). Chemical and biochemical sensors are measuring devices that convert a chemical of a particular analyte into a measurable signal, where the signal is proportional to the concentration of the analyte (12).…”