A fluorescent switchable AIE probe for selective imaging of dipeptidyl peptidase-4 in vitro and in vivo and its application in screening DPP-4 inhibitors

Wang, Yi; Wu, Xueli; Cheng, Yiyu; Zhao, Xiaoping

doi:10.1039/c5cc08921b

Cited by 40 publications

(28 citation statements)

References 26 publications

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“…In addition to the above-mentioned AIE bioprobes, many other excellent AIEgen bioconjugates based on enzyme cleavage that were not elaborated on in this mini-review are summarized in Table 2 with information about their target enzymes, associated diseases, respective AIEgen bioconjugates for detection of target enzyme and their responsive peptide sequences. [49][50][51][52][53][54] 3.2.2 AIEgen-carbohydrate bioconjugate. Interestingly, there are several reported AIEgen-carbohydrates that have shown applicability in the sensing of glycoside hydrolase enzyme through the same enzyme cleavage mechanism applied in AIEgen-peptide probes.…”

Section: Aiegen Bioconjugate Probe Based On Enzyme Cleavagementioning

confidence: 99%

AIEgen bioconjugates for specific detection of disease-related protein biomarkers

Chong

Liu

2019

Mater. Chem. Front.

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Section: Aiegen Bioconjugate Probe Based On Enzyme Cleavagementioning

confidence: 99%

AIEgen bioconjugates for specific detection of disease-related protein biomarkers

Chong

Liu

2019

Mater. Chem. Front.

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“…Thus, CLNPPPV/BDP is useful for in vivo inflammation imaging and diagnosing inflammation‐related disease. In addition, by exploiting the AIE effect, Zhao et al have developed a new TPE‐KFPE probe to screen drugs for type 2 diabetes mellitus in zebrafish …”

Section: Lighting Up Living Bodies and Benefiting Healthmentioning

confidence: 99%

Aggregation‐Induced Emission: Lighting up Cells, Revealing Life!

Wang

Zhang

Liang

2016

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115

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Understanding metabolism and dynamic biological events in cells, as well as physiological functions and pathological changes in organisms, is the major goal of biological investigations. It will improve our capability to diagnose and treat diseases, and will enhance personalized medicine. Fluorescence imaging is a powerful tool that plays an essential role in acquiring the comprehensive knowledge necessary to help reach this goal. Fluorescent molecules are crucial factors for obtaining high quality images. In contrast to conventional fluorogens with aggregation-caused quenching (ACQ) effect, molecules that show aggregation-induced emission (AIE) effect open up new avenues for fluorescence imaging. So far, a large variety of AIE probes have been developed and applied to bioimaging because of their outstanding characteristics, such as high fluorescence efficiency, excellent photostability and high signal-to-noise ratio (SNR). In this review, recent advances in AIE-based probes for biomedical imaging of intracellular microenvironments, natural macromolecules, subcellular organelles, intracellular processes, living tissues, and diagnosis and therapeutic evaluation of diseases in vivo are summarized. It is hoped that this review generates great research enthusiasm for AIE-based bioimaging, in order to promote the development of promising AIE probes and guide us to a better understanding of the biological essence of life.

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“…A variety of AIE-functionalized probes based on enzyme-actived cleavage reactions have been developed via a general assay, and these fluorescent turn-on bioprobes can be used for monitoring matrix metallopeptidase 13 [31], dipeptidyl peptidase-4 [32], γ-glutamyl transpeptidase [33], chymase [34], angiotensin converting enzyme [35], caspase-3 [36][37][38][39], real-time imaging cancer cell apoptosis [40][41][42][43][44], differentiation in living stem cells [45] and targeted drug delivery and therapeutic [46].…”

Section: Amido Bondmentioning

confidence: 99%

Analyte-responsive fluorescent probes with AIE characteristic based on the change of covalent bond

Wang

et al. 2019

Sci. China Mater.

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It is important for the determination of biologically and/or environmentally relevant species by utilization of fluorescent probes. Conventional fluorescent probes are subjected to the influence of aggregation-caused quenching that is limiting their application due to low selectivity as well as photobleaching. Additionally, quencher pairs are usually introduced in the design of these probes, which lead to the complex synthetic procedure. A novel class of fluorogens with aggregation-induced emission (AIE) characteristic provide a solution to address the dilemma. By taking advantage of the unique characteristic of AIE fluorogens, specific turn-on probes have been developed via combination with recognition components, exhibiting low background, good selectivity and outstanding photostability. This review focuses on the development of fluorescent probes with AIE characteristics via the bond cleavage as well as formation strategy.

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A fluorescent switchable AIE probe for selective imaging of dipeptidyl peptidase-4 in vitro and in vivo and its application in screening DPP-4 inhibitors

Cited by 40 publications

References 26 publications

AIEgen bioconjugates for specific detection of disease-related protein biomarkers

AIEgen bioconjugates for specific detection of disease-related protein biomarkers

Aggregation‐Induced Emission: Lighting up Cells, Revealing Life!

Analyte-responsive fluorescent probes with AIE characteristic based on the change of covalent bond

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