AIEgen bioconjugates for specific detection of disease-related protein biomarkers

Abstract

Mis-regulation of certain proteins and enzymes is often identified as an indicator of many pathologies, and these disease-related proteins can be used as biomarkers to diagnose disease onset and progression.

One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens

“…31,32 To date, AIEgens have enjoyed great successes in bioanalyte sensing with the merits of low background, high sensitivity and good photobleaching resistance. [33][34][35][36][37][38][39] The multirotor structures of AIEgens also endow them with high sensitivity to the surrounding microenvironment. In particular, when bearing twisted D-A structures, AIEgens can adapt different molecular congurations and show diverse uorescence color responses to the microenvironments based on the TICT effect.…”

In vivomonitoring of tissue regeneration using a ratiometric lysosomal AIE probe

“…In recent decades, AIEgens have been proved to possess high photostability, large Stokes shi, excellent capability of longterm tracking and good biocompatibility. [55][56][57][58][59][60][61][62][63][64][65][66][67] Although great attention has been paid to tissue regeneration and lysosome study, 4,5,27,28,[68][69][70] monitoring lysosomal pH during tissue regeneration in vivo has not been investigated yet.…”

Enzyme and AIEgens Modulated Solid‐State Nanochannels: In Situ and Noninvasive Monitoring of H2O2 Released from Living Cells

“…This new strategy aims to detect H 2 O 2 released from living cells based on functionalized solid‐state nanochannels without living cells insertion procedure. Aggregation‐induced emission luminogens (AIEgens, a class of luminogens, which are emitting strong fluorescence in aggregated state) with enzyme‐responsive linkage property were utilized in this strategy . In this fabrication, living cells are first incubated on the transparent and flexible polyethylene terephthalate (PET) membrane for 24 h. The PET membrane (thickness: 12 µm) is then rolled up and inserted into left chamber of nanochannel device (Scheme b).…”

“…Aggregationinduced emission luminogens (AIEgens, a class of luminogens, which are emitting strong fluorescence in aggregated state) with enzyme-responsive linkage property were utilized in this strategy. [61][62][63][64][65][66][67][68] In this fabrication, living cells are first incubated on the transparent and flexible polyethylene terephthalate (PET) membrane for 24 h. The PET membrane (thickness: 12 µm) is then rolled up and inserted into left chamber of nanochannel device (Scheme 1b). The solid-state nanochannels that functionalized with capture probes are mounted between two halves of the conductance cell.…”