In vivomonitoring of tissue regeneration using a ratiometric lysosomal AIE probe

Abstract

An AIE-active ratiometric probe for the first time achieved the long-term quantification of lysosomal pH during the medaka larva's caudal fin regeneration.

Fluorescent small organic probes for biosensing

“…Recently, in 2020 the Tang group proposed an AIE lysosome targeting probe for monitoring lysosomal pH during the tissue regeneration process. 115 Lysosomes play a key role in tissue regeneration, for which pH 4–5 is essential for lysosomal hydrolase activity. Whilst research has been conducted on lysosomes and tissue regeneration, an in vivo study of lysosome pH during the regeneration process had not previously been reported.…”

Materials with aggregation-induced emission characteristics for applications in diagnosis, theragnosis, disease mechanism study and personalized medicine

“…al recently reported the utilization of an AIEgen for imaging the tissue regeneration process. 82 In their approach, lysosome was selected as the target, due to its important roles in autophagy, a critical process involved in tissue regeneration. To track the lysosome and quantify lysosomal pH, CSMPP was designed and synthesized ( Figure 17A).…”

Strategic engineering of alkyl spacer length for a pH-tolerant lysosome marker and dual organelle localization

“…37,38 Due to the dynamicity of the lysosomal compartment, imaging lysosomal properties to comprehend its activities is extremely fascinating and to pursue this, numerous synthetic organic lysotrackers have been reported by various research groups. [38][39][40][41] The majority of such reported lysosome targeting probes and commercially available lysotrackers like Neutral Red (NR), DND-189, and DND-99 suffer from pHdependent fluorescence, interference from non-specific binding, background fluorescence, insufficient photostability, and cytotoxicity. 26,42,43 .…”