Materials with aggregation-induced emission characteristics for applications in diagnosis, theragnosis, disease mechanism study and personalized medicine

Zhao, Engui; Chen, Sijie

doi:10.1039/d0qm01132k

Cited by 27 publications

(13 citation statements)

References 85 publications

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“…Organic fluorescent dyes have been generally applied to biological imaging, fluorescent probes, pathological detection, and other fields on account of their strong fluorescence emission [1][2][3][4]. However, traditional organic fluorescent dyes often show an aggregation-caused quenching (ACQ) phenomenon, which severely limits their practical application.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of New AIEE-Active Chalcones for Imaging of Mitochondria in Living Cells and Zebrafish In Vivo

Luo

Liu

et al. 2021

IJMS

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Fluorophores with aggregation-induced emission enhancement (AIEE) properties have attracted increasing interest in recent years. On the basis of our previous research, we successfully designed and synthesized eleven chalcones. Through an optical performance experiment, we confirmed that compounds 1–6 had obvious AIEE properties. As these AIEE molecules had excellent fluorescence properties and a large Stokes shift, we studied their application in living cell imaging, and the results showed that these compounds had low cytotoxicity and good biocompatibility at the experimental concentrations. More importantly, they could specifically label mitochondria. Subsequently, we selected zebrafish as experimental animals to explore the possibilities of these compounds in animal imaging. The fluorescence imaging of zebrafish showed that these AIEE molecules can enter the embryo and can be targeted to aggregate in the digestive tract, which provides a strong foundation for their practical application in the field of biological imaging. Compared with traditional fluorophores, these AIEE molecules have the advantages of possessing a small molecular weight and high flexibility. Therefore, they have excellent application prospects in the field of biological imaging. In addition, the findings of this study have very positive practical significance for the discovery of more AIEE molecules.

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Section: Introductionmentioning

confidence: 99%

Synthesis of New AIEE-Active Chalcones for Imaging of Mitochondria in Living Cells and Zebrafish In Vivo

Luo

Liu

et al. 2021

IJMS

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“…The quantum yields of TPD, TPD-C6, and TPD-C12 in solution are less than 0.1%, and their quantum yields in solid are around 5%. Differing from common applications of AIEgens using the aggregation-induced emission mechanism, , cell membrane requires to be lighted up via a restriction-induced emission process, where an AIE-active probe becomes emissive in the restricted microenvironment of cell membranes. We first used adamantane as a solid matrix to examine the restriction-induced emission behaviors of these compounds, and Figure b shows that their red emissions basically remained with small alternations in emission maximum when they are homogeneously dispersed in adamantane compared to their pure solids, but it is easily noticed that their emissions become brighter in adamantane than in pure solid, indicating that the emission brightness from restriction-induced emission is more effective or comparable to that of aggregation-induced emission.…”

Section: Resultsmentioning

confidence: 99%

Antipermeability Strategy to Achieve Extremely High Specificity and Ultralong Imaging of Diverse Cell Membranes Based on Restriction-Induced Emission of AIEgens

et al. 2022

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Long-term in situ cell membrane-targeted bioimaging is of great significance for studying specific biological processes and functions, but currently developed membrane probes are rarely simultaneously used to image the plasma membrane of animal and plant cells, and these probes lack sufficiently high long-term targeting ability. Herein, we proposed an antipermeability strategy to achieve highly specific and long-term imaging of plasma membranes of both human and plant cells using the steric hindrance effect and restriction-induced emission of AIE-active probes based on an updated membrane model. A certain degree of rigidity of plasma membrane containing a large ratio of rigid cholesterol molecules in the updated membrane model provides a promising opportunity to design antipermeable probes by introducing a rigid steric hindrance group in the probe. The designed antipermeable probes can anchor inside plasma membrane for a long term relying on the combination of the steric hindrance effect and the electrostatic and hydrophobic interactions between the probe and the membrane, as well as light up the membrane via the restriction-induced emission mechanism. The excellent performance in imaging completeness and specificity for both human cells and plant cells clearly shows that these designed probes possess outstanding antipermeability to achieve long-term specific imaging of membrane. These probes also show some advanced features such as ultrafast staining, wash-free merit, favorable biocompatibility, good photostability, and effective resistance to viscosity and pH alteration. This work also provides a valuable design principle for membrane probes of plant cells that the designed probes require a suitable molecular size favoring the penetration of small pores of cell walls.

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“…Materials with aggregation-induced emission (AIE) characteristics are gaining increasing attention, and they have been employed as PSs for microorganisms and tumor treatments due to their superior advantages of good photostability and enhanced ROS production in the aggregated state. [33][34][35][36][37][38][39] Previously, we reported an AIE-active PS, DTTPB (Figure 1a), for efficient inactivation of human coronavirus with PDT. [40] Encouraged by these exciting results, we further explored its potential for simultaneous photodynamic eradication of biofilm and tooth whitening (Figure 1b).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Photodynamic Eradication of Tooth Biofilm and Tooth Whitening with an Aggregation‐Induced Emission Luminogen

Jiang

et al. 2022

Advanced Science

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Dental caries is among the most prevalent dental diseases globally, which arises from the formation of microbial biofilm on teeth. Besides, tooth whitening represents one of the fastest‐growing areas of cosmetic dentistry. It will thus be great if tooth biofilm eradication can be combined with tooth whitening. Herein, a highly efficient photodynamic dental therapy strategy is reported for tooth biofilm eradication and tooth discoloration by employing a photosensitizer (DTTPB) with aggregation‐induced emission characteristics. DTTPB can efficiently inactivate S. mutans, and inhibit biofilm formation by suppressing the expression of genes associated with extracellular polymeric substance synthesis, bacterial adhesion, and superoxide reduction. Its inhibition performance can be further enhanced through combined treatment with chlorhexidine. Besides, DTTPB exhibits an excellent tooth‐discoloration effect on both colored saliva‐coated hydroxyapatite and clinical teeth, with short treatment time (less than 1 h), better tooth‐whitening performance than 30% hydrogen peroxide, and almost no damage to the teeth. DTTPB also demonstrates excellent biocompatibility with neglectable hemolysis effect on mouse red blood cells and almost no killing effect on mammalian cells, which enables its potential applications for simultaneous tooth biofilm eradication and tooth whitening in clinical dentistry.

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Materials with aggregation-induced emission characteristics for applications in diagnosis, theragnosis, disease mechanism study and personalized medicine

Abstract: Investigations on healthcare-related subjects are becoming increasingly popular among scientists, focusing on the early diagnosis of diseases, innovations in disease treatments and gaining mechanistic insights into the occurrence, development and...

Cited by 27 publications

References 85 publications

Synthesis of New AIEE-Active Chalcones for Imaging of Mitochondria in Living Cells and Zebrafish In Vivo

Synthesis of New AIEE-Active Chalcones for Imaging of Mitochondria in Living Cells and Zebrafish In Vivo

Antipermeability Strategy to Achieve Extremely High Specificity and Ultralong Imaging of Diverse Cell Membranes Based on Restriction-Induced Emission of AIEgens

Simultaneous Photodynamic Eradication of Tooth Biofilm and Tooth Whitening with an Aggregation‐Induced Emission Luminogen

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