A fluorescent modification of the Sakaguchi reaction on arginine

“…Further, improvement was made by introducing a little modification in Sakaguchi reaction for quantitative determination of L -arginine using sulfosalicylic acid and oxine solution with hypobromite, it shows better specificity to arginine with other amino acid but arginine derived materials like methyl arginine also shows good response with this methods [30] . Notenboon et al [31] proposed fluorescent modification of the Sakaguchi reaction using 2,4 dichloro α-naphthol for reaction with arginine and it exhibited good response with higher sensitivity. Many others analytical methods using chromatographic technology were applied to determine the arginine content.…”

Section: Introductionmentioning

confidence: 99%

L-arginine biosensors: A comprehensive review

Verma

Singh

2017

Biochemistry and Biophysics Reports

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Arginine has been considered as the most potent nutraceutics discovered ever, due to its powerful healing property, and it's been known to scientists as the Miracle Molecule. Arginine detection in fermented food products is necessary because, high level of arginine in foods forms ethyl carbamate (EC) during the fermentation process. Therefore, L-arginine detection in fermented food products is very important as a control measure for quality of fermented foods, food supplements and beverages including wine. In clinical analysis arginine detection is important due to their enormous inherent versatility in various metabolic pathways, topmost in the synthesis of Nitric oxide (NO) and tumor growth. A number of methods are being used for arginine detection, but biosensors technique holds prime position due to rapid response, high sensitivity and high specificity. However, there are many problems still to be addressed, including selectivity, real time analysis and interference of urea presence in the sample. In the present review we aim to emphasize the significant role of arginine in human physiology and foods. A small attempt has been made to discuss the various techniques used for development of arginine biosensor and how these techniques affect their performance. The choice of transducers for arginine biosensor ranges from optical, pH sensing, ammonia gas sensing, ammonium ion-selective, conductometric and amperometric electrodes because ammonia is formed as a final product.

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“…Another problem associated with these methods is the severe disturbance caused by contamination with urea and proteins [3,9]. The Sakaguchi reaction and its modifications [10][11][12] are well-known as a colorimetric method for the determination of arginine. However, these methods are time-consuming and are disturbed by substances including Tris, glycine, proteins, and NOTICE: This is the author's version of a work that was accepted for publication in Enzyme and Microbial Technology.…”

Section: Introductionmentioning

confidence: 99%

Rapid enzymatic assays for l-citrulline and l-arginine based on the platform of pyrophosphate detection

Kameya

Asano

2014

Enzyme and Microbial Technology

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Rapid determination of L-citrulline and L-arginine, physiologically important amino acids, is a beneficial technique from the scientific and medical viewpoints. In this study, enzymatic assays for L-citrulline and L-arginine were established and evaluated. L-Citrulline assay was constructed by coupling argininosuccinate synthetase to a pyrophosphate detection system, in which pyruvate phosphate dikinase was employed, so that the citrulline-dependent production of pyrophosphate could be determined. Furthermore, the L-arginine assay was developed by coupling arginine deiminase to the L-citrulline assay. Both assays exhibited high selectivity to L-citrulline and L-arginine without any significant reactivity to other proteinaceous amino acids. These assays were also resistant to various contaminants that interfered with the conventional L-citrulline and L-arginine assays. The high accuracy of these assays was demonstrated by measurements in the presence of human plasma. Because these assays can be conducted under the neutral pH without terminating the reaction progress, they allow not only measurements in static analyte solutions, but also real-time monitoring of L-citrulline and L-arginine synthesis in the reaction mixture. The features of these assays also demonstrated that the pyrophosphate detection system served as a useful platform to develop selective and robust enzymatic assays by being coupled to a pyrophosphate-producing enzyme.

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A fluorescent modification of the Sakaguchi reaction on arginine

Cited by 13 publications

References 8 publications

Deoxyribonucleoprotein Changes in Ram and Bull Spermatids

Deoxyribonucleoprotein Changes in Ram and Bull Spermatids

L-arginine biosensors: A comprehensive review

Rapid enzymatic assays for l-citrulline and l-arginine based on the platform of pyrophosphate detection

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