A fluorescent immunochromatographic strip test using a quantum dot-antibody probe for rapid and quantitative detection of 1-aminohydantoin in edible animal tissues

Abstract

A rapid, simple, and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) has been developed to detect 1-aminohydantoin (AHD), a major metabolite of nitrofurantoin in animal tissues. To achieve this, QD-labeled antibody conjugates, which consist of CdSe/ZnS QDs and monoclonal antibodies, were prepared by an activated ester method. Under optimal conditions, with the nitrophenyl derivative of AHD as the target, the ICST had a linear range from 0.1 to 100 ng/mL, with a correlation coefficient of 0.9656 and a 50% inhibitory concentration of 4.51 ng/mL. The limit of detection was 0.14 ng/g, which was below the minimum required performance limit of 1 μg/kg for AHD established by the European Commission. The recoveries for AHD ranged from 81.5% to 108.2%, with coefficients of variation below 13%, based on intraday and interday analysis. Furthermore, for AHD in real samples, the ICST showed high reliability and high correlation with liquid chromatography-tandem mass spectrometry (correlation coefficient of 0.9916). To the best of our knowledge, this is the first report of a novel and sensitive method based on a fluorescent ICST to detect AHD below the minimum required performance limit. The ICST demonstrated high reliability, and could be ideally suited for rapid, simple, and on-site screening of AHD contamination in animal tissues. Graphical abstract A rapid, simple, and sensitive fluorescent immunochromatographic strip test that is based on quantum dots was developed to detect 1-aminohydantoin (AHD), a major metabolite of nitrofurantoin in animal tissues. 2-NBA 2-nitrobenzaldehyde, NP nitrophenyl.

Immunosensor‐based rapid quantitative detection of Newcastle disease virus antibodies using innovative gold immunochromatographic assay

“…2013; Le et al . 2018) a two‐reaction system and double‐test lines (T1, T2) to improve the accuracy, including rabbit IgG with Gold–goat anti‐rabbit IgG conjugate and NDV antibody–HN with Gold‐HN conjugate for the control line (C) and double‐test lines (T1, T2), respectively (Fig. 1a,b).…”

Progress in immunoassays for nitrofurans detection

“…The recovery fell in the range of 76.3-98.4%, and the coefficient of variation was less than 15.0% (Le et al, 2016). The same group subsequently applied the QD-ICA technique to the detection of AHD (Le et al, 2018), and achieved a LOD of 0.14 μg/kg, which was below the MRPL of 1 μg/kg for AHD set by the European Commission. This method offered a fast and simple on-site detection of the desired analyte.…”

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

“…In a Cd-negative sample, the fluorescently labelled mAb (FM-mAb) binds to the coating antigen Cd-ITCBE-BSA at the T line. The remaining unbound FM-mAb migrates upward along the NC membrane and binds to the goat anti-mouse secondary antibody where it reaches the C line (Le, Zhang, Wu, Shi, & Cao, 2018). In a Cd-positive sample, FM-mAb binds to Cd, and unreacted FM-mAb and conjugate migrate along the NC membrane.…”