A flow cytometric study of hydrodynamic damage to mammalian cells

Al‐Rubeai, Mohamed; Emery, A. N.; Chalder, S.; Goldman, M.

doi:10.1016/0168-1656(93)90158-j

Cited by 51 publications

(26 citation statements)

References 21 publications

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“…The maximum cell concentration decreased concurrently with increasing aeration rate ( Table 2). Similar observations were also reported by Al-Rubeai et al [27], who recommended that high hydrodynamic stress which is caused by heavy agitation or bubble bursting, may lead to the aggressive and rapid destruction of the cells just after the beginning of cell death. , respectively (Fig.…”

Section: Resultssupporting

confidence: 77%

Cultivation and comparison of BHK-21 anchorage semi-dependent cell line in different production systems

Ayyildiz-Tamis¹,

Nalbantsoy²,

Elibol³

et al. 2012

Turk J Bioch

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Aim: The investigation of the different culture systems in order to get high yield of baby hamster kidney (BHK) cell line. Materials and Methods: Different cultivation systems, vibrofermentor and 5-L stirred tank bioreactors, have been used for the production of BHK cell line. The production systems were evaluated with respect to pH, temperature, viable cell number, aeration rate and stirring speed. Results: BHK cells were successfully cultivated in vibrofermentor and stirred tank bioreactor (STR) systems. In addition, higher cell concentration was obtained with low aeration rate (0.005 vvm) comparing to those of 0.02 and 0.05 vvm. The cultivation in STR can be operated with low aeration rate due to decrease the expenditure and improve the cell productivity. Conclusion: In order to improve the cell yield of the anchorage semi-dependent cell line in pilot scale, it should be preferred that the cells are firstly adapted to suspension culture by vibrational stirring and then be cultivated in stirred tank bioreactor which is operated at low aeration rate. These results showed consistency with the previous studies. Key Words: BHK-21, stirred tank bioreactor, vibrofermentor Conflict of Interest: Authors have no conflict of interests. ÖZETAmaç: Bebek hamster böbrek (BHK) hücre hattında, hücre veriminin arttırılması ve pilot ölçekte elde edilmesi için üretim sistemlerinin incelenmesi. Gereç ve Yöntem: Bu çalışmada, BHK hücre hattının üretimi vibrofermentör ve 5 L karıştırmalı tank biyoreaktör kültür sistemleri kullanılarak gerçekleştirildi. Üretim sistemlerinin, pH, sıcaklık, canlı hücre derişimi, havalandırma ve karıştırma hızı gibi parametreleri değerlendirildi. Bulgular: BHK hücrelerinin, hem vibrofermentörde hem de karıştırmalı tank biyoreaktörde başarılı bir şekilde üretilebildiği görüldü. Ayrıca, 0.02 ve 0.05 vvm havalandırma hızlarında elde edilenlere göre düşük havalandırma hızında (0.005 vvm) daha yüksek hücre konsantrasyonlarına ulaşıldı. Düşük havalandırma hızlarında çalışan karıştırmalı tank biyoreaktörün hem maliyet hem de hücre verimliliği açısından daha uygun olduğu belirlendi. Sonuçlar: Yarı-yüzey bağımlı BHK hücrelerinin pilot ölçekli üretiminde yüksek verim elde edebilmek için, öncelikle vibrasyon türü karıştırma ile hücrelerin süspansiyon kültüre adaptasyonu ve ardından düşük havalandırma hızlarında çalıştırılan karıştırmalı tank biyoreaktörlerin kullanılması tercih edilebilir. Elde edilen bu sonuçlar daha önceki çalışmalar ile paralellik göstermektedir. Anahtar Kelimeler: BHK-21, karıştırmalı tank biyoreaktör, vibrofermentör. Çıkar Çatışması: Yazarların çıkar çatışması bulunmamaktadır.

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Section: Resultssupporting

confidence: 77%

Cultivation and comparison of BHK-21 anchorage semi-dependent cell line in different production systems

Ayyildiz-Tamis¹,

Nalbantsoy²,

Elibol³

et al. 2012

Turk J Bioch

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“…The most widely used mitochondrial-specific probe, rhodamine 123 (R123), is a cationic compound that accumulates in the mitochondria as a function of transmembrane potential. 79 It was historically applied to spermatozoa in combination with ethidium bromide. 80 The R123 accumulates in the mitochondria and fluoresces green, the fluorescence intensity depends on total amount of functioning mitochondria.…”

Section: Sperm Intactnessmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

135

108

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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“…However, such adjustments are not practical in mammalian cell cultures [25]. This is due to the fragile nature of animal cells, which can be damaged under conditions of high turbulence [26,27]. Hence, in mammalian cell culture the objectives of maximizing oxygen transfer rates must be balanced with the maintenance of the integrity of the cell membrane [28,29].…”

Section: Discussionmentioning

confidence: 99%

Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension

Negrete¹,

Ling²,

Lyddiatt³

2008

TOBIOTJ

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A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium.

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A flow cytometric study of hydrodynamic damage to mammalian cells

Cited by 51 publications

References 21 publications

Cultivation and comparison of BHK-21 anchorage semi-dependent cell line in different production systems

Cultivation and comparison of BHK-21 anchorage semi-dependent cell line in different production systems

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension

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