A fibrinogen-based precision microporous scaffold for tissue engineering

Linnes, Michael P.; Ratner, Buddy D.; Giachelli, Cecilia M.

doi:10.1016/j.biomaterials.2007.08.020

Cited by 158 publications

(161 citation statements)

References 31 publications

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“…However, researchers have tried to develop methods by which they can precisely control the nano-and microstructure of fabricated fibrin scaffolds. Linnes et al 185 developed a method by which fibrin scaffolds of defined pore size, porosity, and microstructure can be fabricated. Such procedures involved pouring a fibrinogen and thrombin solution around an interconnected template made of PMMA beads.…”

Section: Application Of Nanotechnology In Fibrin-based Bone Tissue Enmentioning

confidence: 99%

“…After polymerization of fibrin, the PMMA beads were dissolved in acetone through several rinses using acetone and the scaffolds were resolvated in 70% ethanol overnight. 185 Next, the authors incorporated calcium phosphate nanoparticles into the matrices and observed that in vitro culturing of osteoblast-like cells on modified scaffolds resulted in significantly higher ALP activity as well as osteoblast marker gene expression compared to fibrin scaffolds, while higher cell proliferation was detected on fibrin alone scaffolds. An in vivo study was performed by the implantation of disc-shaped scaffolds in cranial defects of mice, and the results demonstrated the promotion of bone formation in defects treated with fibrin/calcium phosphate scaffolds.…”

Section: Application Of Nanotechnology In Fibrin-based Bone Tissue Enmentioning

confidence: 99%

See 1 more Smart Citation

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

344

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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Section: Application Of Nanotechnology In Fibrin-based Bone Tissue Enmentioning

confidence: 99%

Section: Application Of Nanotechnology In Fibrin-based Bone Tissue Enmentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

344

256

View full text Add to dashboard Cite

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“…Some instability issues can be counteracted with a higher concentration of polymer in the gel, but attention has to be paid to the resulting pore size. Higher concentrated gels form a dense network which impedes cell migration and ingrowth into the matrix [23,24]. Lower concentrated gels have poor mechanical properties and may tear easily during implantation.…”

Section: Modifications For Enhanced Stabilitymentioning

confidence: 99%

Hydrogels based on collagen and fibrin – frontiers and applications

Schneider-Barthold¹,

Baganz²,

Wilhelmi

et al. 2016

BioNanoMaterials

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Hydrogels are a versatile tool for a multitude of applications in biomedical research and clinical practice. Especially collagen and fibrin hydrogels are distinguished by their excellent biocompatibility, natural capacity for cell adhesion and low immunogenicity. In many ways, collagen and fibrin represent an ideal biomaterial, as they can serve as a scaffold for tissue regeneration and promote the migration of cells, as well as the ingrowth of tissues. On the other hand, pure collagen and fibrin materials are marked by poor mechanical properties and rapid degradation, which limits their use in practice. This paper will review methods of modification of natural collagen and fibrin materials to next-generation materials with enhanced stability. A special focus is placed on biomedical products from fibrin and collagen already on the market. In addition, recent research on the in vivo applications of collagen and fibrin-based materials will be showcased.

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“…This is because; fibrin shows excellent properties as the matrix where it has been widely used to investigate the angiogenic behavior of different types of endothelial cells in vitro with the presence of growth factor [20]. Fibrin is one of the natural proteins that shows to support the vascular growth meanwhile another synthetic materials are effectively inhibit angiogenesis unless they are impregnated with growth factor [21].…”

Section: Extra-cellular Matrix (Ecm) For Angiogenesis Studymentioning

confidence: 99%