A Fiber-Optic Cocaine Biosensor

Devine, Patrick J.; Anis, Nabil A.; Wright, J.R.; Kim, Sung Hoon; Eldefrawi, Amira T.; Eldefrawi, Mohyee E.

doi:10.1006/abio.1995.1273

Cited by 46 publications

(18 citation statements)

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“…Finally, we anticipate that the optimized displacement method (coupled with QCM) and model for TNT detection can be modified for the detection of other molecules of interest (e.g. imazethapyr [49] or cocaine [50]), provided that an antibody specific for the molecule of interest, and displaying cross reactivity for analogous molecules that typically limit its detection, has been isolated or engineered.…”

Section: Discussionmentioning

confidence: 99%

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Wang

AlMakhaita²,

Biswal³

et al. 2012

J Biosens Bioelectron

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Currently available TNT sensors are characterized by high sensitivity, but low specificity, which limits the detection of TNT in dirty environments. We report here a TNT sensor designed to measure the displacement of a TNT-specific antibody by quartz crystal microbalance (QCM). This sensor combines high sensitivity of detection (0.1 ng/mL) with the ability to distinguish TNT from molecules with similar chemical properties. Particularly, the reliability of this method for the detection of TNT in dirty environments was investigated by using fertilizer solution and artificial seawater. Instead of measuring actual binding of TNT, the method described is based on the displacement of an anti-TNT antibody, which allows quantifying the concentration of TNT in solution with higher sensitivity. In addition, by utilizing the rate of antibody displacement, the detection time is significantly decreased from hours, which would be necessary to measure the frequency change at equilibrium, to minutes. A Langmuir kinetic model was used to describe the molecular interactions on the surface of the sensor and to establish a standard curve to estimate on-site TNT detection. In summary, QCM detection of anti-TNT antibody displacement provides a method for rapid detection of TNT with high sensitivity and specificity.

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Section: Discussionmentioning

confidence: 99%

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Wang

AlMakhaita²,

Biswal³

et al. 2012

J Biosens Bioelectron

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“…13 ) The purpose of this study was to develop a rapid, sensitive, and easy-to-use fiber-optic biosensor for the detection of protein A, which is an indicator of the presence of S. aureus.…”

Section: Detection Of Protein a Produced By Staphylococcus Aureus Witmentioning

confidence: 99%

Detection of Protein A Produced byStaphylococcus aureuswith a Fiber-optic-based Biosensor

Chang¹,

Chang

Kao³

et al. 1996

Bioscience, Biotechnology, and Biochemistry

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Staphylococcus aureus is a pathogen important in causing human infections and intoxication. A sensitive fiber-optic that produces evanescent waves was developed for the detection of protein A, a product secreted only by S. aureus. In the immunosensor, a 40-mV argon-ion laser that generated laser light at 488 nm was used together with plastic optical fiber and antibodies to protein A were physically adsorbed onto the fiber. The principle of the detection involved a sandwich immunoassay with fluorescein isothiocyanate conjugated with anti-(protein A) immunoglobulin G to produce signals of the antigen-antibody reaction. The detection limit was 1 ng of protein A per milliliter. The fiber-optic immunosensor could be used for rapid and specific detection of S. aureus in clinical specimens and foods.

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“…Nowadays, the advances in designing and fabricating micro-cantilever devices capable of detecting extremely small forces, mechanical stresses offer the promising prospect of biological sensing with unprecedented sensitivity [3]. So, in the last several years, there has been great interest in bio-and immunosensors that employ micro-cantilever sensors to detect specific disease markers [4]. Traditional cantilever-based immunosensor is achieved by immobilizing a monolayer of receptor molecules on one side of the cantilever and then detecting the mechanical bending induced by antigen-antibody specific binding in a liquid environment.…”

Section: Introductionmentioning

confidence: 99%

Design and optimization of micro-cantilever-based and magnetic bead-based immunosensor for application of the biological molecules testing

Gao

Yao

et al. 2006

2006 8th International Conference on Solid-State and Integrated Circuit Technology Proceedings

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A Fiber-Optic Cocaine Biosensor

Cited by 46 publications

References 0 publications

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Detection of Protein A Produced byStaphylococcus aureuswith a Fiber-optic-based Biosensor

Design and optimization of micro-cantilever-based and magnetic bead-based immunosensor for application of the biological molecules testing

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