Detection of Protein A Produced by<i>Staphylococcus aureus</i>with a Fiber-optic-based Biosensor

Chang, Yu-Hao; Chang, Tsung Chain; Kao, E-Fong; Chou, Chien

doi:10.1271/bbb.60.1571

Cited by 41 publications

(2 citation statements)

References 6 publications

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“…Rapid detection and identification of S. aureus bacteremia facilitates a prompt and adequate antibiotic therapy. Protein A is a potent virulence factor and can be taken as indicating the presence of S. aureus in clinical specimens [ 28 , 29 ]. Most S. aureus strains from clinical sources contained protein A associated with the cell wall or released extracellularly [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

et al. 2020

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Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

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Section: Discussionmentioning

confidence: 99%

Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

et al. 2020

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“…A similar LOD value of 1.56 pg/mL was only obtained with a chemiluminescence enzyme immunoassay (CLEIA) based system ( Fujimoto et al, 2008 ). The presented LSPCF fiber-optic biosensor presents many interesting and useful features, as it measures the fluorescence signal close to the reaction region resulting in a significant increase of the fluorescence efficiency ( Chang et al, 1996 ), showing also a high specificity, thanks to the sandwich immunoassay configuration.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Developments in biosensors for CoV detection and future trends

Antiochia

2021

Biosensors and Bioelectronics

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This review summarizes the state of art of biosensor technology for Coronavirus (CoV) detection, the current challenges and the future perspectives. Three categories of affinity-based biosensors (ABBs) have been developed, depending on their transduction mechanism, namely electrochemical, optical and piezoelectric biosensors. The biorecognition elements include antibodies and DNA, which undergo important non-covalent binding interactions, with the formation of antigen-antibody and ssDNA/oligonucleotide-complementary strand complexes in immuno- and DNA-sensors, respectively. The analytical performances, the advantages and drawbacks of each type of biosensor are highlighted, discussed, and compared to traditional methods. It is hoped that this review will encourage scientists and academics to design and develop new biosensing platforms for point-of-care (POC) diagnostics to manage the coronavirus disease 2019 (COVID-19) pandemic, providing interesting reference for future studies.

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Biosensors

Turner

Laschi

Mascini

2002

Kirk-Othmer Encyclopedia of Chemical Technology

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Biosensors are chemical sensors in which the recognition system utilizes a biochemical mechanism. The term biosensor has been variously used to a number of devices either used to monitor living system or incorporating biotic elements. The current consensus is that this term should be reserved for sensors incorporating a biological element such as an enzyme, antibody, nucleic acid, microorganism, or eukariotic cell where the biological element is in intimate contact with the transducer. The main purpose of the recognition system is to provide specificity to the biosensor, thus creating a device able to detect either a specific molecular target or a related family of compounds. The ability of biosensors to detect trace and low levels of biologically active substances is the main characteristic of these systems, and for this reason they have applications in the fields of medicine, biotechnology, food, and environmental science.

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Detection of Protein A Produced byStaphylococcus aureuswith a Fiber-optic-based Biosensor

Cited by 41 publications

References 6 publications

Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Developments in biosensors for CoV detection and future trends

Biosensors

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