A feasibility study of the use of a SIRE biosensor in the monitoring of H2O2 added to milk samples. A possible new technology for early detection of mastitis?

Leth, Suzanne; Krook, Margareta; Hörnsten, E. Gunnar

doi:10.1016/j.ifset.2004.06.004

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…Barkema et al (1998) indicated that management practices such as dry cow treatment, milking technique, post milking teat disinfection, and antibiotic treatment of clinical mastitis were found to be important in the explanation of the difference among SCC levels. Besides, the average of untransformed SCC value estimated in the study (302772 cells/ml) was within the acceptable level for human consumption according to EU directive (92/46/EEC) which prescribed a threshold of 400x10 3 cells/ml (Leth et al 2004). …”

Section: Resultssupporting

confidence: 63%

Influence of some environmental factors on body condition score and somatic cell count in Czech Holstein cows

Stádník

Atasever

2015

IJAR

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The objective of this study was to determine some environmental factors affecting body condition score (BCS) and somatic cell count (SCC) in Czech Holstein cows. A total of 278 primiparous cows were examined by BCS and SCC in four calving seasons, three years and six test days after calving. The results revealed no significant effect of calving season was found for both parameters. While year was not effective on SCC values, BCS mean of third year was significantly (P<0.05) high. Also, no significant effect of test day was determined on SCC, but BCS values elevated with later test days (P<0.05). Negative and significant relationships (P<0.01) were estimated among all consecutive change in BCS (CBCS) and positive associations (P<0.01) were determined among all logSCC groups. As the result, primiparous Czech Holstein cows should be managed by eliminating effective factors to obtain more productivity in dairy farms.

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Section: Resultssupporting

confidence: 63%

Influence of some environmental factors on body condition score and somatic cell count in Czech Holstein cows

Stádník

Atasever

2015

IJAR

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“…According the data published in the literature the catalase content increases in the mastitic milk up to 5–10 U mL −1 25. Therefore, we added different amounts of catalase enzyme (2, 5, 10 U mL −1 ) to 0.2 and 0.1 mM hydrogen peroxide standard.…”

Section: Resultsmentioning

confidence: 99%

Development of a Catalase‐Based Amperometric Biosensor for the Determination of Increased Catalase Content in Milk Samples

et al. 2011

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The aim of our work was to develop a selective amperometric biosensor for infected milk detection. The catalase enzyme (EC 1.11.1.6) was immobilized onto the surface of a thin-layer enzyme cell. The measurements were carried out in stopped-flow injection analysis system (0.8 mL min À1 flow rate, + 590 mV polarization potential) in organic phase (6 % sodium acetate buffer 200 mM pH 6.0, ferrocene conductive salt (7.5 mg L À1 ) dissolved in acetonitrile. A certain amount of hydrogen peroxide was degraded by the catalase content of the milk. The degradation of the hydrogen peroxide is proportional to the infection of the milk samples.

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A feasibility study of the use of a SIRE biosensor in the monitoring of H2O2 added to milk samples. A possible new technology for early detection of mastitis?

Cited by 2 publications

References 23 publications

Influence of some environmental factors on body condition score and somatic cell count in Czech Holstein cows

Influence of some environmental factors on body condition score and somatic cell count in Czech Holstein cows

Development of a Catalase‐Based Amperometric Biosensor for the Determination of Increased Catalase Content in Milk Samples

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