A feasibility study of a leaky waveguide aptasensor for thrombin

Alamrani, Nasser A.; Greenway, Gillian M.; Pamme, Nicole; Goddard, N J

doi:10.1039/c9an01421g

Cited by 11 publications

(7 citation statements)

References 42 publications

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“…The deposition of the metal layer increases cost by requiring vacuum deposition and makes the integration of electric fields with LWs challenging [2]. The waveguide resonances have also been visualised by immobilizing suitable dyes in the waveguide, resulting in dips in reflectivity [7][8][9][10]. The immobilization of the dye reduces the number of available binding sites, thus reducing sensitivity, and increases non-specific absorption of sample components.…”

Section: Introductionmentioning

confidence: 99%

A novel manifestation at optical leaky waveguide modes for sensing applications

Goddard

2020

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

A novel manifestation at optical leaky waveguide modes for sensing applications

Goddard

2020

Sensors and Actuators B: Chemical

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“…Aptamers attach to their targets specifically via hydrogen bonding, electrostatic, and hydrophobic interactions. The presented aptasensor had high durability, low cost, and a simple manufacturing procedure [ 134 ].…”

Section: Optical Biosensorsmentioning

confidence: 99%

Recent Progresses in Development of Biosensors for Thrombin Detection

et al. 2022

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Thrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, which causes blood clots to prevent bleeding. The rapid and sensitive detection of thrombin is important in biological analysis and clinical diagnosis. Hence, various biosensors for thrombin measurement have been developed. Biosensors are devices that produce a quantifiable signal from biological interactions in proportion to the concentration of a target analyte. An aptasensor is a biosensor in which a DNA or RNA aptamer has been used as a biological recognition element and can identify target molecules with a high degree of sensitivity and affinity. Designed biosensors could provide effective methods for the highly selective and specific detection of thrombin. This review has attempted to provide an update of the various biosensors proposed in the literature, which have been designed for thrombin detection. According to their various transducers, the constructions and compositions, the performance, benefits, and restrictions of each are summarized and compared.

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“…Equally, the authors pioneered the application of hydrogel thin films to make diffraction-based optical leaky waveguide (LW) biosensors 6,7 . In diffraction-based LWs, resonance angles are observed directly as exponentially decaying bright and dark fringes without having to use dyes 8 , metal films 9 , and patterned structures 10 . Exponentially decaying bright and dark fringes are observed because of the interference between light that was uncoupled and coupled in the hydrogel film with cylindrical and plane wavefronts, respectively 7 .…”

Section: Introductionmentioning

confidence: 99%