A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota

Coyne, Michael J.; Béchon, Nathalie; Matano, Leigh M.; McEneany, Valentina Laclare; Chatzidaki‐Livanis, Maria; Comstock, Laurie E.

doi:10.1038/s41467-019-11494-1

Cited by 55 publications

(53 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…may utilize various transporters (such as ABC exporters) to actively secrete the toxins to the environment of their microbial targets. 38 Several of the diffusible toxins produced by Bacteroides spp. have membrane attack complex/perforin (MACPF) domains.…”

Section: Antimicrobial Toxins Are Used In Bacterial Competitionmentioning

confidence: 99%

Gut Bacteroides species in health and disease

2021

View full text Add to dashboard Cite

The functional diversity of the mammalian intestinal microbiome far exceeds that of the host organism, and microbial genes contribute substantially to the well-being of the host. However, beneficial gut organisms can also be pathogenic when present in the gut or other locations in the body. Among dominant beneficial bacteria are several species of Bacteroides, which metabolize polysaccharides and oligosaccharides, providing nutrition and vitamins to the host and other intestinal microbial residents. These topics and the specific organismal and molecular interactions that are known to be responsible for the beneficial and detrimental effects of Bacteroides species in humans comprise the focus of this review. The complexity of these interactions will be revealed.

show abstract

Section: Antimicrobial Toxins Are Used In Bacterial Competitionmentioning

confidence: 99%

Gut Bacteroides species in health and disease

2021

View full text Add to dashboard Cite

show abstract

“…Accordingly, these common predominant bacteria also played a crucial role in the host's nutrient absorption and immune defense, such as synthesizing glycoside hydrolase and polysaccharide lyase, promoting the degradation of the fibrosome, fructose, starch, etc., and providing the five-carbon or six-carbon sugars [36,38]; the bacterial components (such as LPS [39]) and metabolites (such as SCFAs [40,41], antimicrobial peptides [6,42], etc.) were important in the regulating host energy metabolism and intestinal development and maintaining intestinal epithelial integrity etc.…”

Section: The Relationship Between the Host And Common Dominant Bacteriamentioning

confidence: 99%

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

Zhang

Yao

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

The microbial communities colonize the mucosal immune inductive sites could be captured by hosts, which could initiate the mucosal immune responses. The aggregated lymphoid nodule area (ALNA) and the ileal Payer's patches (PPs) in Bactrian camels are both the mucosal immune inductive sites of the gastrointestinal tract. Here, the bacteria community associated with the ALNA and ileal PPs were analyzed using of 16S rDNA-Illumina Miseq sequencing. The mutual dominant bacterial phyla at the two sites were the Bacteroidetes, Firmicutes, Verrucomicrobia and Proteobacteria, and the mutual dominant genus in both sits was Prevotella. The abundances of the Fibrobacter, Campylobacter and RFP12 were all higher in ALNA than in ileal PPs. While, the abundances of the 5-7N15, Clostridium, and Escherichia were all higher in ileal PPs than in ALNA. The results suggested that the host's intestinal microenvironment is selective for the symbiotic bacteria colonizing the corresponding sites, on the contrary, the symbiotic bacteria could impact on the physiological functions of this local site. In ALNA and ileal PPs of Bactrian camel, the bacteria which colonized different immune inductive sites have the potential to stimulate different immune responses, which is the result of the mutual selection and adaptation between microbial communities and their host.

show abstract

“…Analyses of recent human fecal isolates of Bacteroidales with interesting phenotypes revealed that many are resistant to both tetracycline and erythromycin (60, 65) and therefore are not amenable to genetic manipulation using available genetic tools for these bacteria. We evaluated tetracycline and erythromycin resistance among 120 Bacteroides strains from our collection isolated between 2009 and 2011 from the stool of 15 healthy U.S.-resident adults with no history of gastrointestinal diseases (Fig.…”

Section: Resultsmentioning

confidence: 99%

Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota

García-Bayona

Comstock

2019

mBio

Self Cite

View full text Add to dashboard Cite

Studies of the gut microbiota have dramatically increased in recent years as the importance of this microbial ecosystem to human health and disease is better appreciated. The Bacteroidales are the most abundant order of bacteria in the healthy human gut and induce both health-promoting and disease-promoting effects. There are more than 55 species of gut Bacteroidales with extensive intraspecies genetic diversity, especially in regions involved in the synthesis of molecules that interact with other bacteria, the host, and the diet. This property necessitates the study of diverse species and strains. In recent years, the genetic toolkit to study these bacteria has greatly expanded, but we still lack a facile system for creating deletion mutants and allelic replacements in diverse strains, especially with the rapid increase in resistance to the two antibiotics used for genetic manipulation. Here, we present a new versatile and highly efficient vector suite that allows the creation of allelic deletions and replacements in multiresistant strains of Bacteroides and Parabacteroides using a gain-of-function system based on polysaccharide utilization. These vectors also allow for easy counterselection independent of creating a mutant background strain, using a toxin from a type VI secretion system of Bacteroides fragilis. Toxin production during counterselection is induced with one of two different molecules, providing flexibility based on strain phenotypes. This family of vectors greatly facilitates functional genetic analyses and extends the range of gut Bacteroidales strains that can be genetically modified to include multiresistant strains that are currently genetically intractable with existing genetic tools. IMPORTANCE We have entered an era when studies of the gut microbiota are transitioning from basic questions of composition and host effects to understanding the microbial molecules that underlie compositional shifts and mediate health and disease processes. The importance of the gut Bacteroidales to human health and disease and their potential as a source of engineered live biotherapeutics make these bacteria of particular interest for in-depth mechanistic study. However, there are still barriers to the genetic analysis of diverse Bacteroidales strains, limiting our ability to study important host and community phenotypes identified in these strains. Here, we have overcome many of these obstacles by constructing a series of vectors that allow easy genetic manipulation in diverse gut Bacteroides and Parabacteroides strains. These constructs fill a critical need and allow streamlined allelic replacement in diverse gut Bacteroidales, including the growing number of multiantibiotic-resistant strains present in the modern-day human intestine.

show abstract

A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota

Cited by 55 publications

References 60 publications

Gut Bacteroides species in health and disease

Gut Bacteroides species in health and disease

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota

Contact Info

Product

Resources

About