Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

Zhang, Wangdong; Yao, Wan-Ling; He, Wei; Li, Jianfei; Wu, Xiuping; Chen, Zhihua; Liu, Lei; Wang, Wenhui

doi:10.1371/journal.pone.0239987

Cited by 4 publications

(5 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The intestinal immune system often nourishes prosperous bacterial communities and establishes advanced symbiotic relationships ( Sutherland & Fagarasan, 2012 ). Firmicutes, Bacteroidetes, and Proteobacteria were the three main phyla across all the samples in this study, which is consistent with our previous study that found that these phyla dominantly colonized on the surface of the different mucosal immune inductive sites of the gastrointestinal tract in Bactrian camels ( Zhang et al, 2020 ). Proteobacteria is conducive to the homeostasis of the anaerobic environment of the GI tract gut ( Moon et al, 2018 ), while the ratio of Firmicutes and Bacteroidetes impacts gut dysbiosis ( Grigor’eva, 2020 ).…”

Section: Discussionsupporting

confidence: 92%

“…Recently, more research has been devoted to revealing the internal interactions between gut taxa and intestinal mucosa. By comparing commensal microbial composition differences between the aggregated lymphoid nodule area (ALNA) and the ileal PPS, we previously observed that the host’s intestinal microenvironment is selective for the symbiotic bacteria colonizing the corresponding sites, which could impact the physiological functions of the site ( Zhang et al, 2020 ). However, it is still unknown if there are associations between colonized flora and the gut-expressed genes in PPS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Integrated omics analysis reveals the immunologic characteristics of cystic Peyer’s patches in the cecum of Bactrian camels

Wang

et al. 2023

PeerJ

View full text Add to dashboard Cite

Bactrian camels have specific mucosa-associated lymphoid tissue (MALT) throughout the large intestine, with species-unique cystic Peyer’s patches (PPS) as the main type of tissue. However, detailed information about the molecular characteristics of PPS remains unclear. This study applied a transcriptomic analysis, untargeted metabolomics, and 16S rDNA sequencing to compare the significant differences between PPS and the adjacent normal intestine tissues (NPPS) during the healthy stage of three young Bactrian camels. The results showed that samples from PPS could be easily differentiated from the NPPS samples based on gene expression profile, metabolites, and microbial composition, separately indicated using dimension reduction methods. A total of 7,568 up-regulated and 1,266 down-regulated differentially expressed genes (DEGs) were detected, and an enrichment analysis found 994 DEGs that participated in immune-related functions, and a co-occurance network analysis identified nine hub genes (BTK, P2RX7, Pax5, DSG1, PTPN2, DOCK11, TBX21, IL10, and HLA-DOB) during multiple immunologic processes. Further, PPS and NPPS both had a similar pattern of most compounds among all profiles of metabolites, and only 113 differentially expressed metabolites (DEMs) were identified, with 101 of these being down-regulated. Deoxycholic acid (DCA; VIP = 37.96, log2FC = −2.97, P = 0), cholic acid (CA; VIP = 13.10, log2FC = −2.10, P = 0.01), and lithocholic acid (LCA; VIP = 12.94, log2FC = −1.63, P = 0.01) were the highest contributors to the significant dissimilarities between groups. PPS had significantly lower species richness (Chao1), while Firmicutes (35.92% ± 19.39%), Bacteroidetes (31.73% ± 6.24%), and Proteobacteria (13.96% ± 16.21%) were the main phyla across all samples. The LEfSe analysis showed that Lysinibacillus, Rikenellaceae_RC9_gut_group, Candidatus_Stoquefichus, Mailhella, Alistipes, and Ruminococcaceae_UCG_005 were biomarkers of the NPPS group, while Escherichia_Shigella, Synergistes, Pyramidobacter, Odoribacter, Methanobrevibacter, Cloacibacillus, Fusobacterium, and Parabacteroides were significantly higher in the PPS group. In the Procrustes analysis, the transcriptome changes between groups showed no significant correlations with metabolites or microbial communities, whereas the alteration of metabolites significantly correlated with the alteration of the microbial community. In the co-occurrence network, seven DEMs (M403T65-neg, M329T119-neg, M309T38-neg, M277T42-2-neg, M473T27-neg, M747T38-1-pos, and M482t187-pos) and 14 genera (e.g., Akkermansia, Candidatus-Stoquefichus, Caproiciproducens, and Erysipelatoclostridium) clustered much more tightly, suggesting dense interactions. The results of this study provide new insights into the understanding of the immune microenvironment of the cystic PPS in the cecum of Bactrian camels.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Integrated omics analysis reveals the immunologic characteristics of cystic Peyer’s patches in the cecum of Bactrian camels

Wang

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

“…In our previous study, we found the bacterial communities to be similar between the RMFR and LMFR (50). The similar bacterial structure suggests that these differences may not be caused by bacterial parasitism, but rather to the host's internal expression, which implies distinct physiological patterns for RMFR and LMFR.…”

Section: Discussionmentioning

confidence: 71%

Proteomic analysis reveals similarity of expression profile in aggregated lymphoid nodules in the abomasum with Peyer’s patches of Bactrian camel

Cheng

Ren

Wang

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Aggregated lymphoid nodules area (ALNA) is a distinctive anatomical feature of the abomasum in Bactrian camels, consisting of the reticular mucosal folds region (RMFR) and the longitudinal mucosal folds region (LMFR). ALNA exhibits many of the same characteristics histologically as Peyer's patches (PPs) in the intestinal tract. We analyzed the functional characteristics of ALNA in gastrointestinal mucosal immunity. Results We quantified the expressed proteins in ALNA using iTRAQ-based proteomic analysis in six Bactrian camels. In the experiment, we sampled the RMFR and LMFR separately from the ALNA and compared their proteomic quantification results with samples from the PPs. A total of 1790 proteins were identified, among which only 22 differentially expressed proteins (DEPs) were found between RMFR and PPs, 35 DEPs were found between LMFR and PPs, and 19 DEPs were found between LMFR and RMFR. There was no direct correlation between DEPs and immune-related functions. Subsequently, the abundance changes of 10 DEPs were verified at the transcriptional level by qPCR. Such as TPM1, MYH11 and HSPB1 were significantly up-regulated in LMFR vs PPs, but down-regulated in RMFR vs PPs. The expression levels of CR2 and GALS2 were found to be significantly increased in RMFR vs PPs, respectively, while decreased in LMFR. The expression of 10 genes was largely consistent with the corresponding protein expression. Besides, InnateDB was used to identify information on immune-associated proteins in ALNA, over 87.7% of proteins have been recognized in the same orthogroups of a human immune-related gene. Those proteins are recognized as involving a variety of functions, including the treatment and presentation of antigens, the intracellular activation of T-cells, the T-cell and B-cell receptor pathways, and the regulation of actin polycondensation. Conclusions The experimental findings indicate that the immune-related proteins present in ALNA and PPs exhibit similarities. Despite differences in structural phenotypes between LMFR and RMFR, proteins expressed in the two structures are highly similar and play similar roles in mucosal immune responses.

show abstract

“…In our previous study, we found the bacterial communities to be similar between the RMFR and LMFR [ 54 ]. The similar bacterial structure suggests that these differences may not be caused by bacterial parasitism, but rather to the host’s internal expression, which implies distinct physiological patterns for RMFR and LMFR.…”

Section: Discussionmentioning

confidence: 93%

Similar proteome expression profiles of the aggregated lymphoid nodules area and Peyer’s patches in Bactrian camel

Cheng,

Ren,

Wang

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

Background The presence of Aggregated Lymphoid Nodules Area (ALNA) is a notable anatomical characteristic observed in the abomasum of Bactrian camels. This area is comprised of two separate regions, namely the Reticular Mucosal Folds Region (RMFR) and the Longitudinal Mucosal Folds Region (LMFR). The histological properties of ALNA exhibit significant similarities to those of Peyer’s patches (PPs) found in the gastrointestinal system. The functional characteristics of ALNA were examined in relation to mucosal immunity in the gastrointestinal system. Results We used iTRAQ-based proteomic analysis on twelve Bactrian camels to measure the amount of proteins expressed in ALNA. In the experiment, we sampled the RMFR and LMFR separately from the ALNA and compared their proteomic quantification results with samples from the PPs. A total of 1253 proteins were identified, among which 39 differentially expressed proteins (DEPs) were found between RMFR and PPs, 33 DEPs were found between LMFR and PPs, and 22 DEPs were found between LMFR and RMFR. The proteins FLNA, MYH11, and HSPB1 were chosen for validation using the enzyme-linked immunosorbent assay (ELISA), and the observed expression profiles were found to be in agreement with the results obtained from the iTRAQ study. The InnateDB database was utilized to get data pertaining to immune-associated proteins in ALNA. It was observed that a significant proportion, specifically 76.6%, of these proteins were found to be associated with the same orthogroups as human immune-related genes. These proteins are acknowledged to be associated with a diverse range of functions, encompassing the uptake, processing and presentation of antigens, activation of lymphocytes, the signaling pathways of T-cell and B-cell receptors, and the control of actin polymerization. Conclusions The experimental results suggest that there are parallels in the immune-related proteins found in ALNA and PPs. Although there are variations in the structures of LMFR and RMFR, the proteins produced in both structures exhibit a high degree of similarity and perform comparable functions in the context of mucosal immune responses.

show abstract

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

Cited by 4 publications

References 51 publications

Integrated omics analysis reveals the immunologic characteristics of cystic Peyer’s patches in the cecum of Bactrian camels

Integrated omics analysis reveals the immunologic characteristics of cystic Peyer’s patches in the cecum of Bactrian camels

Proteomic analysis reveals similarity of expression profile in aggregated lymphoid nodules in the abomasum with Peyer’s patches of Bactrian camel

Similar proteome expression profiles of the aggregated lymphoid nodules area and Peyer’s patches in Bactrian camel

Contact Info

Product

Resources

About