A facile method for the fabrication of magnetic molecularly imprinted stir-bars: A practical example with aflatoxins in baby foods

Díaz-Bao, Mónica; Regal, Patricia; Barreiro, Rocío; Fente, C.; Cepeda, Alberto

doi:10.1016/j.chroma.2016.10.022

Cited by 46 publications

(22 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MIPs, in contrast to classical SPE sorbents, are more selective and allow the elution of analytes from the cartridges, virtually free from co-extracted compounds ( Figure 2) [26]. Díaz-Bao et al [28] developed a quick and easy method for the fabrication of magnetic molecularly imprinted stir-bars (MMIP-SB). They used a combination of imprinting technology and magnetite, for the analysis of AFM1 in milk powder (infant formulas).…”

Section: Extractionmentioning

confidence: 99%

Detection Methods for Aflatoxin M1 in Dairy Products

et al. 2020

View full text Add to dashboard Cite

Mycotoxins are toxic compounds produced mainly by fungi of the genera Aspergillus, Fusarium and Penicillium. In the food chain, the original mycotoxin may be transformed in other toxic compounds, reaching the consumer. A good example is the occurrence of aflatoxin M1 (AFM1) in dairy products, which is due to the presence of aflatoxin B1 (AFB1) in the animal feed. Thus, milk-based foods, such as cheese and yogurts, may be contaminated with this toxin, which, although less toxic than AFB1, also exhibits hepatotoxic and carcinogenic effects and is relatively stable during pasteurization, storage and processing. For this reason, the establishment of allowed maximum limits in dairy products and the development of methodologies for its detection and quantification are of extreme importance. There are several methods for the detection of AFM1 in dairy products. Usually, the analytical procedures go through the following stages: sampling, extraction, clean-up, determination and quantification. For the extraction stage, the use of organic solvents (as acetonitrile and methanol) is still the most common, but recent advances include the use of the Quick, Easy, Cheap, Effective, Rugged, and Safe method (QuEChERS) and proteolytic enzymes, which have been demonstrated to be good alternatives. For the clean-up stage, the high selectivity of immunoaffinity columns is still a good option, but alternative and cheaper techniques are becoming more competitive. Regarding quantification of the toxin, screening strategies include the use of the enzyme-linked immunosorbent assay (ELISA) to select presumptive positive samples from a wider range of samples, and more reliable methods—high performance liquid chromatography with fluorescence detection or mass spectroscopy—for the separation, identification and quantification of the toxin.

show abstract

Section: Extractionmentioning

confidence: 99%

Detection Methods for Aflatoxin M1 in Dairy Products

et al. 2020

View full text Add to dashboard Cite

show abstract

“…However, the diverse drawbacks of this method (poor stability of patulin under alkaline extraction, poor resolution between patulin and co-extracted hydroxymethylfurfural) have originated interest in alternative options, such as LC methods coupled to mass spectrometry [1]. In the last years, purification with molecularly imprinted polymers and magnetic materials started to be used and they are becoming promising materials in analytical chemistry and, more specifically, in mycotoxins determination [7,8,12,13]. Imprinted polymers have also showed potential for detoxification purposes in large-scale environmental applications [14].…”

Section: Resultsmentioning

confidence: 99%

“…Template bleeding may be an additional problem of these polymers, especially when dealing with very low detection levels. To overcome these limitations, dummy templates can be applied during MIP synthesis [7]. Also, magnetic materials can provide fast and simple methods of extraction and have already demonstrated their effectiveness to extract patulin [8].…”

Section: Introductionmentioning

confidence: 99%

Design of a Molecularly Imprinted Stir-Bar for Isolation of Patulin in Apple and LC-MS/MS Detection

et al. 2017

Self Cite

View full text Add to dashboard Cite

Abstract:Mycotoxins are a very diverse group of natural products produced as secondary metabolites by fungi. Patulin is produced by mold species normally related to vegetable-based products and fruit, mainly apple. Its ingestion may result in agitation, convulsions, edema, intestinal ulceration, inflammation, vomiting, and even immune, neurological or gastrointestinal disorders. For this reason, the European Commission Regulation (EC) 1881/2006 established a maximum content for patulin of 10 ppb in infant fruit juice, 50 ppb for fruit juice for adults and 25 ppb in fruit-derived products. In this work, a rapid and selective method based on magnetic molecularly imprinted stir-bar (MMISB) extraction has been developed for the isolation of patulin, using 2-oxindole as a dummy template. The final extraction protocol consisted of simply pouring in, stirring and pouring out samples and solvents from a beaker with the MMISB acting inside. The magnetic device provided satisfactory recoveries of patulin (60%-70%) in apple samples. The successful MMISB approach has been combined with high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to determine patulin.

show abstract

“…For this reason, the development of new coating materials has become an important issue to improve SBSE methodologies. In this sense, Diaz-Bao et al proposed the synthesis of a magnetic dummy MIP stir bar to achieve the SBSE extraction of 5 aflatoxins from milk and cereal-based baby foods combined with LC-MS/MS analysis [81]. Fe 3 O 4 nanoparticles were first prepared by a co-precipitation method and afterwards they were embedded into a dummy MIP through a bulk polymerization process.…”

Section: Stir-bar Sorptive Extraction (Sbse)mentioning

confidence: 99%

“…Steps of fabrication and application of the magnetic molecularly imprinted stir-bars (reprinted with permission from reference[81]). …”

mentioning

confidence: 99%

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

2020

View full text Add to dashboard Cite

Natural toxins are chemical substances that are not toxic to the organisms that produce them, but which can be a potential risk to human health when ingested through food. Thus, it is of high interest to develop advanced analytical methodologies to control the occurrence of these compounds in food products. However, the analysis of food samples is a challenging task because of the high complexity of these matrices, which hinders the extraction and detection of the analytes. Therefore, sample preparation is a crucial step in food analysis to achieve adequate isolation and/or preconcentration of analytes and provide suitable clean-up of matrix interferences prior to instrumental analysis. Current trends in sample preparation involve moving towards “greener” approaches by scaling down analytical operations, miniaturizing the instruments and integrating new advanced materials as sorbents. The combination of these new materials with sorbent-based microextraction technologies enables the development of high-throughput sample preparation methods, which improve conventional extraction and clean-up procedures. This review gives an overview of the most relevant analytical strategies employed for sorbent-based microextraction of natural toxins of exogenous origin from food, as well as the improvements achieved in food sample preparation by the integration of new advanced materials as sorbents in these microextraction techniques, giving some relevant examples from the last ten years. Challenges and expected future trends are also discussed.

show abstract

A facile method for the fabrication of magnetic molecularly imprinted stir-bars: A practical example with aflatoxins in baby foods

Cited by 46 publications

References 43 publications

Detection Methods for Aflatoxin M1 in Dairy Products

Detection Methods for Aflatoxin M1 in Dairy Products

Design of a Molecularly Imprinted Stir-Bar for Isolation of Patulin in Apple and LC-MS/MS Detection

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

Contact Info

Product

Resources

About