A dual enhanced anti-bacterial strategy based on high chlorin e6-loaded polyethyleneimine functionalized graphene

Wang, Jiangxia; Yang, Yuting; Xu, Yuanliang; Zhao, Lifeng; Wang, Lu; Yin, Zheng‐Zhi; Li, Huiming; Tan, Huan; Liu, Kunping

doi:10.1039/d0ra07976f

Cited by 6 publications

(9 citation statements)

References 23 publications

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“…Liposomes and serum albumin nanocarriers are another example of nanotechnology-based PS applications, and in those studies, hypericin improved the APDT performance and speed [ 168 , 169 ]. Graphene interactions with chlorin PS improved antibacterial efficiency [ 170 ], and hypocrellin A micelles exhibit improved solubility and overcome the solubility problem of APDT [ 171 ].…”

Section: Emerging Technologies and Solutions To Current Apdt Limitationsmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

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Health problems and reduced treatment effectiveness due to antimicrobial resistance have become important global problems and are important factors that negatively affect life expectancy. Antimicrobial photodynamic therapy (APDT) is constantly evolving and can minimize this antimicrobial resistance problem. Reactive oxygen species produced when nontoxic photosensitizers are exposed to light are the main functional components of APDT responsible for microbial destruction; therefore, APDT has a broad spectrum of target pathogens, such as bacteria, fungi, and viruses. Various photosensitizers, including natural extracts, compounds, and their synthetic derivatives, are being investigated. The main limitations, such as weak antimicrobial activity against Gram-negative bacteria, solubility, specificity, and cost, encourage the exploration of new photosensitizer candidates. Many additional methods, such as cell surface engineering, cotreatment with membrane-damaging agents, nanotechnology, computational simulation, and sonodynamic therapy, are also being investigated to develop novel APDT methods with improved properties. In this review, we summarize APDT research, focusing on natural photosensitizers used in in vitro and in vivo experimental models. In addition, we describe the limitations observed for natural photosensitizers and the methods developed to counter those limitations with emerging technologies.

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Section: Emerging Technologies and Solutions To Current Apdt Limitationsmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

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“…This value demonstrated that the selected photosensitizer was effectively adsorbed onto H-CeO 2 NPs. The generation of singlet oxygen ( 1 O 2 ) radicals by exposing H-CeO 2 @Ce6@PDA@GOx NPs to visible light irradiation obtained from a red LED at 650 nm was followed by a spectrophotometric protocol, using DPBF as the fluorescent probe . The variation of DPBF concentration with the time in nonaqueous dispersion of H-CeO 2 @Ce6@PDA@GOx NPs exposed to visible light irradiation was determined by plotting the maximum absorbance of the supernatant obtained from the sample at 410 nm against time (Figure A).…”

Section: Resultsmentioning

confidence: 99%

“…54 Typically, H-CeO 2 NPs (5.0 mg/mL) were mixed with Ce6-methanol solution (1.0 mg/mL, 1.0 mL). The adsorption of Ce6 onto H-CeO 2 NPs was carried out at 55 For this purpose, the adsorption medium was centrifuged at 6000 rpm for 10 min, and the absorbance of the supernatant was measured at 400 nm in a UV−vis spectrophotometer (Thermo Scientific, Genesys 150, USA). Ce6 adsorption onto H-CeO 2 NPs, shown by Q Ce6 , was calculated based on the following equation:…”

Section: Preparation Of Ce6mentioning

confidence: 99%

In Vitro Synergistic Photodynamic, Photothermal, Chemodynamic, and Starvation Therapy Performance of Chlorin e6 Immobilized, Polydopamine-Coated Hollow, Porous Ceria-Based, Hypoxia-Tolerant Nanozymes Carrying a Cascade System

Süngü Akdogan,

Akbay Çetin,

Onur

et al. 2024

ACS Appl. Bio Mater.

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A synergistic therapy agent (STA) with photothermal, photodynamic, chemodynamic, and starvation therapy (PTT, PDT, CDT, and ST) functions was developed. Hollow, mesoporous, and nearly uniform CeO 2 nanoparticles (H-CeO 2 NPs) were synthesized using a staged shape templating sol−gel protocol. Chlorin e6 (Ce6) was adsorbed onto H-CeO 2 NPs, and a thin polydopamine (PDA) layer was formed on Ce6-adsorbed H-CeO 2 NPs. Glucose oxidase (GOx) was bound onto PDA-coated Ce6-adsorbed H-CeO 2 NPs to obtain the targeted STA (H-CeO 2 @Ce6@PDA@GOx NPs). A reversible photothermal conversion behavior with the temperature elevations up to 34 °C was observed by NIR laser irradiation at 808 nm. A cascade enzyme system based on immobilized GOx and intrinsic catalase-like activity of H-CeO 2 NPs was rendered on STA for enhancing the effectiveness of PDT by elevation of ROS generation and alleviation of hypoxia in a tumor microenvironment. Glucose-mediated generation of highly toxic hydroxyl radicals ( • OH) was evaluated for CDT. The effectiveness of PDT on glioblastoma T98G cells was markedly enhanced by O 2 generation started by the decomposition of glucose. A similar increase in cell death was also observed when ST and CDT functions were enhanced by photothermal action. The viability of T98G cells decreased to 10.6% by in vitro synergistic action including ST, CDT, PDT, and PTT without using any antitumor agent.

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“…The time of serially passaged bacteria in penicillin was recorded as a control. 59 Cytotoxicity In Vitro. Cytocompatibility evaluations of hydrogels were performed by the MTT method on L929 cells and 3T3 cells and live/dead staining assay on L929 cells.…”

Section: ■ Conclusionmentioning

confidence: 99%

“…By observing the time before the logarithmic phase of bacteria, the drug resistance of bacteria could be evaluated. The time of serially passaged bacteria in penicillin was recorded as a control …”

Section: Experimental Sectionmentioning

confidence: 99%

Photodynamic Alginate Zn-MOF Thermosensitive Hydrogel for Accelerated Healing of Infected Wounds

Zhang

Wang

Xiang

et al. 2023

ACS Appl. Mater. Interfaces

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Antibiotic resistance reduces the effectiveness of infected wound healing, and it is necessary to develop a new strategy to promote infected wound healing without using antibiotics. Here, we develop a Chlorin e6 (Ce6)-loaded zinc-metal−organic framework (MOF) thermosensitive hydrogel (Ce6@MOF-Gel) based on alginate and poly(propylene glycol) 407, which enhances antibacterial effects and promotes infected wound healing by a novel strategy of combining zinc-MOF with photodynamic therapy (PDT). Zinc-MOF can realize acid-responsive release of Ce6 and improve antibacterial performance without drug resistance by destroying the integrity of bacterial cell membranes and enhancing the production of bacterial reactive oxygen species (ROS). Additionally, Ce6@MOF-Gel enhances the stability, solubility, and photodynamic properties of Ce6. More importantly, Ce6@MOF-Gel reduces inflammation and promotes collagen deposition and re-epithelialization to facilitate infected wound healing. Collectively, the photodynamic MOF-based hydrogel provides a new, efficient, and safe way for accelerated healing of infected wounds.

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A dual enhanced anti-bacterial strategy based on high chlorin e6-loaded polyethyleneimine functionalized graphene

Abstract: Dual enhanced anti-bacterial strategy based on a high chlorin e6-loaded polyethyleneimine functionalized graphene nanomaterial.

Cited by 6 publications

References 23 publications

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

In Vitro Synergistic Photodynamic, Photothermal, Chemodynamic, and Starvation Therapy Performance of Chlorin e6 Immobilized, Polydopamine-Coated Hollow, Porous Ceria-Based, Hypoxia-Tolerant Nanozymes Carrying a Cascade System

Photodynamic Alginate Zn-MOF Thermosensitive Hydrogel for Accelerated Healing of Infected Wounds

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