A Drosophila heat shock gene from locus 67B is expressed during embryogenesis and pupation

Pauli, Daniel; Tonka, Chia-Hwa

doi:10.1016/0022-2836(87)90309-3

Cited by 20 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In particular, the seven small heat shock genes clustered at the chromosomal locus 67B (Fig. 1) show a complex pattern of developmental expression (Sirotkin and Davidson 1982;Zimmerman et al 1983;Mason et al 1984;Ayme and Tissikres 1985;Thomas and Lengyel 1986;Pauli and Tonka 1987;Pauli et al 1988). For example, maternally derived mRNAs encoding hsp26,27, and 83 have been found to be present in very young embryos.…”

Section: Introductionmentioning

confidence: 98%

“…The finding that the treatment of Drosophila tissue culture cells or imaginal discs with the moulting hormone beta-ecdysone induces the synthesis of the low molecular weight hsps Black arrows indicate the position and the orientation of the transcription of the small hsp genes and open arrows those of genes 1, 2, and 3. All these genes but gene 2 have a unique start of transcription and are homologous between themselves and with mammalian alpha-crystallins (Ingolia and Craig 1982;Southgate et al 1983;Ayme and Tissikres 1985;Pauli and Tonka 1987). Gene 2 is interrupted by introns and produces, during different developmental stages, two transcripts differing by the presence or not of a 5' non-coding exon ).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Expression of the small heat shock genes during Drosophila development: comparison of the accumulation of hsp23 and hsp27 mRNAs and polypeptides

Pauli¹,

Arrigo²,

Vazquez³

et al. 1989

Genome

Self Cite

View full text Add to dashboard Cite

Seven heat shock genes are clustered within 15 kilobases of DNA at the Drosophila melanogaster chromosomal site 67B. They show a complex pattern of expression in the absence of external stress during normal development of this organism. In this paper, we quantitatively compare the abundance of the messenger RNAs for these seven genes at all major stages of Drosophila development and then focus on hsp23 and hsp27 for which available antibodies allow the comparison between the accumulation of the mRNAs and that of their corresponding polypeptides. Transcripts for both genes are maximally abundant in white prepupae. We observe that the amount of hsp23 message decreases more rapidly than that of hsp27 mRNA throughout the pupal period. The maximal abundance of the proteins occurs at the middle of the pupal stage, when their corresponding RNAs have almost completely disappeared. The peaks of expression of the proteins are also broader than those of their transcripts, indicating that the half-lives of the polypeptides are longer. These observations suggest that complex mechanisms regulate the expression of the small heat shock genes during Drosophila development.

show abstract

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Expression of the small heat shock genes during Drosophila development: comparison of the accumulation of hsp23 and hsp27 mRNAs and polypeptides

Pauli¹,

Arrigo²,

Vazquez³

et al. 1989

Genome

Self Cite

View full text Add to dashboard Cite

show abstract

“…Based on DNA sequence homology, the genes designated 1 and 3 appear related to the small hsp gene family. Although they are not heat inducible, generally, they do show a pattern of developmental regulation similar to that seen for the small hsp genes (Ayme and Tissieres, 1985;Pauli and Tonka, 1987;Pauli et al, 1988). Gene 1, curiously, is not heat inducible at most stages of development but becomes a heat shock gene during pupation when its developmental expression is maximum.…”

Section: Introductionmentioning

confidence: 90%

“…Recent studies have identified three additional genes interspersed in this 15 kb region (Ayme and Tissieres, 1985;Corces et al, 1980;Craig and McCarthy, 1980;Pauli and Tonka, 1987;Pauli et al, 1988;Voellmy et al, 1981). Based on DNA sequence homology, the genes designated 1 and 3 appear related to the small hsp gene family.…”

Section: Introductionmentioning

confidence: 99%

Regulatory elements near the Drosophila hsp 22 gene required for ecdysterone and heat shock induction

1991

View full text Add to dashboard Cite

A transient expression assay was used to localize cis-acting DNA regulatory elements near the Drosophila heat shock protein (hsp) 22 gene, that are involved in heat shock expression and in ecdysterone-induced expression. The results identify a region between positions -320 and -232 that is essential for ecdysterone control, but not for heat-induced expression, and a sequence between -199 and -156, which, when deleted, leads to the loss of heat shock induction. To investigate the function of these DNA sequences, transfection-competition experiments were carried out. The evidence suggests that the DNA regulatory sequences identified by transient expression studies contain binding sites for transacting transcription factors.

show abstract

“…In addition to being heat inducible, all these genes have been found to be developmentally regulated in a complex manner throughout the life cycle of the fly. In late third instar larvae and prepupae, all four of the small heat shock protein genes and both genes 1 and 3 are expressed (15,18,23) at a time coinciding with elevated titers of the insect steroid molting hormone ecdysterone. Ecdysterone directly stimulates the synthesis of the small heat shock protein gene mRNAs and proteins in both the Schneider 3 (S3) D. melanogaster cell line and isolated imaginal discs (12,13).…”

mentioning

confidence: 99%

Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Kelly

Cartwright

1989

Mol. Cell. Biol.

View full text Add to dashboard Cite

Alterations in the pattern of DNase I hypersensitivity were observed on ecdysterone-stimulated transcription of Drosophila melanogaster small heat shock protein genes. Perturbations were induced near hsp27 and hsp22, coupled with an extensive domain of chromatin unfolding in the intergenic region between hsp23 and the developmentally regulated gene 1. These regions represent candidates for ecdysterone regulatory interactions.

show abstract

A Drosophila heat shock gene from locus 67B is expressed during embryogenesis and pupation

Cited by 20 publications

References 28 publications

Expression of the small heat shock genes during Drosophila development: comparison of the accumulation of hsp23 and hsp27 mRNAs and polypeptides

Expression of the small heat shock genes during Drosophila development: comparison of the accumulation of hsp23 and hsp27 mRNAs and polypeptides

Regulatory elements near the Drosophila hsp 22 gene required for ecdysterone and heat shock induction

Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Contact Info

Product

Resources

About