Regulatory elements near the Drosophila hsp 22 gene required for ecdysterone and heat shock induction

Abstract

A transient expression assay was used to localize cis-acting DNA regulatory elements near the Drosophila heat shock protein (hsp) 22 gene, that are involved in heat shock expression and in ecdysterone-induced expression. The results identify a region between positions -320 and -232 that is essential for ecdysterone control, but not for heat-induced expression, and a sequence between -199 and -156, which, when deleted, leads to the loss of heat shock induction. To investigate the function of these DNA sequences, transfection-competition experiments were carried out. The evidence suggests that the DNA regulatory sequences identified by transient expression studies contain binding sites for transacting transcription factors.

Lifespan extension in C. elegans by a molecular chaperone dependent upon insulin‐like signals

“…Notably, hsp‐16 promoters lack obvious binding sites for DAF‐16, further implicating indirect regulation of hsp‐16 by DAF‐16. The Drosophila homologue of hsp‐16 , hsp22 , is in part regulated by the insect hormone ecdysterone (Rudolph et al ., 1991) and like hsp‐16 of C. elegans , D. melanogaster hsp22 is intrinsically up‐regulated with age (Wheeler et al ., 1995). Similar hormonal regulation of stress genes may exist in the worm.…”