Multiple interacting elements delineate an ecdysone-dependent regulatory region with secondary responsive character

Rogulski, Kenneth; Cartwright, Iain L.

doi:10.1006/jmbi.1995.0298

Cited by 6 publications

(4 citation statements)

References 68 publications

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“…The small hsp genes of Drosophila melanogaster are coordinately induced by heat shock and are subject to complex de-velopmental control, with a major peak of expression at the end of the third larval instar. The nature and location of cisacting regulatory elements involved in the regulation of small hsp genes have been studied in a variety of systems, including heterologous cells, Drosophila tissue culture cells, and P-element-transformed flies (27,32). Discrete heat shock elements and functional EcREs have been localized in short regions flanking the hsp23 transcription unit, and trans-acting DNAbinding proteins that recognize these sites are known.…”

Section: Discussionmentioning

confidence: 99%

The Broad-Complex Gene Is a Tissue-Specific Modulator of the Ecdysone Response of the Drosophila hsp23 Gene

Dubrovsky

Dretzen

Berger

1996

Molecular and Cellular Biology

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The steroid hormone ecdysone causes dramatic changes in the genetic programs leading to the pupariation of Drosophila melanogaster, and the Broad-Complex (BR-C) gene is known to play a key role in this process. Previously we showed that BR-C regulates developmental changes in transcription and chromatin structure of the 67B heat shock gene cluster, which contains four small hsp genes. Importantly, the downregulation of the hsp23 gene in the BR-C mutants correlates with the absence of a DNase I-hypersensitive site (DHS) at position -1400. To study the functional importance of the DHS-1400, we have introduced genomic fragments containing a modified hsp23 gene into the Drosophila germ line. Our analysis shows that the ecdysone response element is necessary but not sufficient for full developmental expression of hsp23 in the late third instar and that there is, indeed, another regulatory element, in the vicinity of DHS-1400. We also show that hsp23 developmental expression is not tissue specific. A construct lacking the ecdysone response element is unable to direct normal hsp23 expression in all tissues except the brain. Similarly, brain-specific expression is BR-C independent, although in the other tissues we find different requirements for BR-C genetic functions. The effect of the br mutations is restricted to wing imaginal discs and midgut tissue, while that of 2Bc is restricted to the fat body and Malpighian tubules, and mutations in the rbp group have no effect in any of the tissues studied. Thus, BR-C regulatory action is mediated through different genetic functions in a tissue-specific manner.

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Section: Discussionmentioning

confidence: 99%

The Broad-Complex Gene Is a Tissue-Specific Modulator of the Ecdysone Response of the Drosophila hsp23 Gene

Dubrovsky

Dretzen

Berger

1996

Molecular and Cellular Biology

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“…Two regions are overlapping with regions earlier found to be important for hormone induction of hsp23 (Mestril et al 1986;Rogulski and Cartwright 1995;Dubrovsky et al 2001) and three regions contain a proneural transcription factor binding site CAGCTG sequence (E-box) (Powell et al 2004). …”

Section: Identification Of Putative Response Element By Comparative Smentioning

confidence: 96%

Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia

Frydenberg

Barker

Loeschcke

2010

Cell Stress and Chaperones

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The small heat shock gene (shsp) cluster of Drosophila buzzatii was sequenced and the gene order and DNA sequence were compared with those of the shsps in Drosophila melanogaster. The D. buzzatii shsp cluster contains an inversion and a duplication of hsp26. A phylogenetic tree was constructed based on hsp26 genes from several Drosophila species of the Sophophora and Drosophila subgenera. The tree shows first a separation of the Sophophora and the Drosophila subgenera and then the Drosophila subgenus is divided into the Hawaiian Drosophila and the repleta/virilis groups. Only the latter contain a duplicated hsp26. Comparing the gene organisation of the shsp cluster shows that all the Drosophila subgenus species contain the inversion. Putative heat shock elements (HSE) were found in the promoters of all the shsp and putative regulator elements for tissue specific expression were found in the promoter of hsp23, hsp27 and one of the hsp26 genes. hsp23 was found to be polymorphic for four non-synonymous changes that all lead to exchange of a Valine. The duplicated hsp26 gene in D. buzzatii (phsp26) was polymorphic for two non-synonymous changes. The allele frequencies of these variants were determined in nine D. buzzatii populations covering most of its distribution in Australia using high-resolution melting curves. The allele frequencies of one of the hsp23 variants showed a significant linear regression with longitude and the pooled frequency of the four Valine changes of hsp23 in the nine populations showed a significant linear regression with longitude and with a composite measure of climatic variables.

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“…Drosophila Schneider Line 2 (SL2) cells were seeded at a density of 3 × 10 6 cells per 35 mm plate in Ex-cell 400 medium supplemented with 6.8 mM glutamine and cultured overnight at 25_C prior to transfection. Triplicate plates were co-transfected with each combination of CAT reporter (600 ng) and the indicated expression plasmid (activator plus empty pac5c-pl vector to total 600 ng) by the DEAE dextran (100 µg/ml) method for 4-5 h at 25_C (35). Transfection medium was removed, the cells washed, re-fed with 2 ml of medium and cultured for 72 h. Cells were then harvested into 1 ml TNE (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA) and spun for 1 min at 13 000 r.p.m.…”

Section: Cloramphenicol Acetyltransferase (Cat) Assaysmentioning

confidence: 99%

“…[ 35 S]methionine labeled Ski and NFI proteins were produced by coupled transcription-translation in vitro using the p5′EETM1 or pRP0.5 plasmids described above according to the suppliers protocol (Promega). Binding of in vitro translated proteins to GST-v-Ski was performed and analyzed as described previously (5).…”

Section: In Vitro Translation Co-immunoprecipitation and Gst-fusion P...mentioning

confidence: 99%

DNA Binding and Transcriptional Activation by the Ski Oncoprotein Mediated by Interaction with NFI

Tarapore

Richmond²,

Zheng

et al. 1997

Nucleic Acids Research

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The Ski oncoprotein has been found to bind non-specifically to DNA in association with unindentified nuclear factors. In addition, Ski has been shown to activate transcription of muscle-specific and viral promoters/enhancers. The present study was undertaken to identify Ski's DNA binding and transcriptional activation partners by identifying specific DNA binding sites. We used nuclear extracts from a v-Ski-transduced mouse L-cell line and selected Ski-bound sequences from a pool of degenerate oligonucleotides with anti-Ski monoclonal antibodies. Two sequences were identified by this technique. The first (TGGC/ANNNNNT/GCCAA) is the previously identified binding site of the nuclear factor I (NFI) family of transcription factors. The second (TCCCNNGGGA) is the binding site of Olf-1/EBF. By electophoretic mobility shift assays we find that Ski is a component of one or more NFI complexes but we fail to detect Ski in Olf-1/EBF complexes. We show that Ski binds NFI proteins and activates transcription of NFI reporters, but only in the presence of NFI. We also find that homodimerization of Ski is essential for co-activation with NFI. However, the C-terminal dimerization domain of c-Ski, which is missing in v-Ski, can be substituted by the leucine zipper domain of GCN4.

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Multiple interacting elements delineate an ecdysone-dependent regulatory region with secondary responsive character

Cited by 6 publications

References 68 publications

The Broad-Complex Gene Is a Tissue-Specific Modulator of the Ecdysone Response of the Drosophila hsp23 Gene

The Broad-Complex Gene Is a Tissue-Specific Modulator of the Ecdysone Response of the Drosophila hsp23 Gene

Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia

DNA Binding and Transcriptional Activation by the Ski Oncoprotein Mediated by Interaction with NFI

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