Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Kelly, Susan E.; Cartwright, Iain L.

doi:10.1128/mcb.9.1.332

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…Certain of the Drosophila small-hsp genes are induced during oogenesis, and members of the small-hsp gene family in lilies are induced during meiosis. For the Drosophila small-hsp genes, elements regulating heat shock induction are widely dispersed and readily separated from those regulating developmental expression (4,7,10,11,15,17,35). In contrast, none of our many HSP26 deletion mutations separated heat shock regulatory elements from developmental regulatory elements.…”

Section: Resultsmentioning

confidence: 99%

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Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock.

Susek

Lindquist

1990

Mol. Cell. Biol.

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hsp26, the small heat shock protein of Saccharomyces cerevisiae, accumulates in response to heat and other types of stress. It also accumulates during the normal course of development, as cells enter stationary phase growth or begin to sporulate (S. Kurtz, J. Rossi, L. Petko, and S. Lindquist, Science 231:1154-1157, 1986). Analysis of deletion and insertion mutations demonstrated that transcriptional control plays a critical role in regulating HSP26 expression. The HSP26 promoter was found to be complex and appears to contain repressing elements as well as activating elements. Several upstream deletion mutations resulted in strong constitutive expression of HSP26. Furthermore, upstream sequences from the HSP26 gene repressed the constitutive expression of a heterologous heat shock gene. We propose that basal repression and heat-induced depression of transcription play major roles in regulating the expression of HSP26. None of the recombinant constructs that we analyzed separated cis-regulatory sequences responsible for heat shock regulation from those responsible for developmental regulation of HSP26. Depression of HSP26 transcription may be the general mechanism of HSP26 induction in yeast cells. This regulatory scheme is very different from that described for the regulation of most other heat shock genes.

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Section: Resultsmentioning

confidence: 99%

“…This result also is unexpected. In the only other well-studied case, that of the Drosophila small-hsp genes, cis-regulatory sequences involved in stress induction are readily separated from those involved in developmental induction (4,7,10,11,15,17,35).…”

mentioning

confidence: 99%

Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock.

Susek

Lindquist

1990

Mol. Cell. Biol.

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“…In a detailed analysis of DNAse I hypersensitivity at locus 67B, Kelly and Cartwright (1989) observed a major alteration of chromatin structure in the 5' regulatory region of gene 1 following exposure of Drosophila cultured cells to ecdysterone, but not after heat shock. Their results suggest that the HSTF alone is unable to bind to its regulatory sites on the gene i promoter region, or to modify the chromatin structure, in contrast to the other heat shock genes.…”

Section: Discussionmentioning

confidence: 99%

Response to heat shock of gene 1, a Drosophila melanogaster small heat shock gene, is developmentally regulated

Vazquez

1991

Molec. Gen. Genet.

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The expression of gene 1, a member of the small heat shock gene family from the Drosophila melanogaster chromosomal locus 67B was studied. In contrast to the other heat shock genes, the response of gene 1 to stress was modulated during development. In the absence of stress, gene 1 was expressed at the beginning of pupation, and at a very low level in adult males. Expression of gene 1 was substantially increased by heat shock in pupae, but was one to two orders of magnitude lower in adults or in embryos. Under the same conditions, hsp70 or hsp26 were induced to similar levels in all stages. This developmental effect could be mimicked in cultured Drosophila cells: expression of gene 1 was stimulated by heat shock in the presence, but not in the absence, of the moulting hormone ecdysterone, while the level of expression of hsp26 and hsp70 in response to heat shock was independent of the presence of the hormone. Thus, the presence and activity of the heat shock transcription factor are not sufficient for the maximal response of gene 1 to stress. These results suggest that the heat shock activator protein requires additional factors, which are developmentally regulated, to activate transcription of gene 1. Furthermore, S1 nuclease mapping analysis revealed several gene 1 mRNA species, which are generated by the use of alternative polyadenylation sites and by the use of differentially regulated transcriptional initiation sites.

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Multiple interacting elements delineate an ecdysone-dependent regulatory region with secondary responsive character

Rogulski

Cartwright

1995

Journal of Molecular Biology

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Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Cited by 8 publications

References 28 publications

Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock.

Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock.

Response to heat shock of gene 1, a Drosophila melanogaster small heat shock gene, is developmentally regulated

Multiple interacting elements delineate an ecdysone-dependent regulatory region with secondary responsive character

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