A Double-Antibody Radioimmunoassay Procedure for Ovine Pituitary Prolactin

Arai, Y.; Lee, T. H.

doi:10.1210/endo-81-5-1041

Cited by 36 publications

(17 citation statements)

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“…Saji (1967) who used the haemagglutination-inhibition assay for measuring prolactin. These results differ substantially from those reported by Arai & Lee (1967) (Johke, 1970) and hand milking in the goat (Hart, 1972) (Bryant & Greenwood, 1968;Davis et al, 1971;Cumming, Brown, Goding, Bryant & Greenwood, 1972) Prolactin levels are expressed in ng/ml plasma. 1965; Bryant, Linzell & Greenwood, 1970;Johke, 1970;Neill, 1970;Butler, 1971;Raud, Kiddy & Odell, 1971;Cumming et al, 1972;Frantz, Kleinberg & Noel, 1972;Fell, Findlay, Cumming & Goding, 1973 (1971) and Fell et al (1972).…”

contrasting

confidence: 96%

Prolactin Release in the Sheep

Ge¹,

Moseley²,

McNeilly³

1974

Reproduction

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The recent development of radioimmunoassay for prolactin has extended the depth and extent of studies of factors affecting prolactin levels in mammals and has permitted long-term studies to be made of changes in blood prolactin levels in individual animals. Although ovine prolactin has been widely studied in relation to its structure (Li, 1972) and biological activity (see Wolstenholme & Knight, 1972) and has been extensively used as a reference material, the serum prolactin levels of the sheep have not been widely investigated. Many of the early studies involved measurements of single samples obtained by venepuncture, a stressful procedure now known to affect prolactin release in the

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confidence: 96%

Prolactin Release in the Sheep

Ge¹,

Moseley²,

McNeilly³

1974

Reproduction

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“…Chemical isolation of prolactin from several animal species has been accomplished. The amino acid sequence of ovine prolactin has been reported (4), and radioimmunoassays for rat (5,6) and for ovine (7,8) prolactin have been developed.…”

Section: Introductionmentioning

confidence: 99%

Human Prolactin: Measurement in Plasma by In Vitro Bioassay

Kleinberg¹,

Frantz²

1971

J. Clin. Invest.

103

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A B S T R A C T Prolactin has been measured in unex-tracted human plasma by a sensitive and specific in vitro bioassay. Secretory activity of breast tissue fragments from mid-pregnant mice, incubated in organ culture with human plasma, serves as the histologic end point.Sensitivity is 5 ng/ml (0.14 mU/ml) or somewhat better for ovine prolactin, and approximately 0.42 mU/ml for prolactin activity of human plasma at the dilutions used in the assay. Human growth hormone as it circulates in blood, like the material extracted from pituitary glands, is strongly lactogenic. Antisera to human growth hormone are capable of completely neutralizing the prolactin effect of large amounts (600 ng/ml) of human growth hormone added to the system. Plasma prolactin activity is less than 0.42 mU/ml in normal men and women. Of 26 patients with nonpuerperal galactorrhea, 14 had elevated prolactin activities ranging from 0.42 to 3.5 mU/ml. Growth hormone levels by radioimmunoassay were far too low, in general, to account for the observed prolactin activity. All of 14 nursing mothers, 1-30 days post partum, had elevated prolactin activity with a mean of 2.29 and a total range of 0.56-4.5 mU/mi.Growth hormone was in the low normal range in all of these subjects. Seven patients on psychoactive drugs of the phenothiazine series similarly had elevated prolactin activity with low growth hormone. Antiserum to human growth hormone, when preincubated with plasma samples from each of these three groups of subjects, produced no significant inhibition of prolactin activity. In two acromegalic patients with markedly elevated growth hormone levels, antiserum to growth hormone produced complete inhibition of prolactin activity in one and partial inhibition in the other. These studies indicate that human growth hormone and human prolactin This work was presented in part at

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“…The antiserum against pituitary prolactin that was studied here and similar antisera prepared in our own laboratory were also low in titre. This was unexpected in view of the ease in which high-titre antisera against ovine prolactin can be raised in guinea pigs and rabbits [1,5], Antisera against amniotic fluid extract or pituitary material bound label led human pituitary prolactin (Lewis). The label could be displaced by HCG, HTSH, HLH and HFSH, and by unlabelled prolactin.…”

Section: Discussionmentioning

confidence: 99%

Radioimmunoassay for Human Pituitary Prolactin, Using Antiserum against an Extract of Human Amniotic Fluid

Cole¹,

Boyns²

1973

Horm Res

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Rabbit antiserum raised against a prolactin-rich extract of human amniotic fluid was used to establish a double-antibody radioimmunoassay for human pituitary prolactin inplasma. The binding of ¹³¹I-labelIed human pituitary prolactin(Lewis) to the antiserum was inhibited by unlabelled prolactin, human chorionic gonadotrophin (HCG), human luteinizing hormone (HLH) and human follicle stimulating hormone (HFSH). Absorption of the antiserum with HCG rendered the assay specific for prolactin no cross-reaction being then observed with HLH, HFSH, human thyroid stimulating hormone, human growth hormone or human chorionic somatomammotrophin. The binding of ¹³¹I-labelled human pituitary prolactin (Friesen) to antibody was not affected by HCG. The concentration of prolactin in plasmas from normal males, pregnant females, non-pregnant females and in patients treated with phenothiazine was measured.

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A Double-Antibody Radioimmunoassay Procedure for Ovine Pituitary Prolactin

Cited by 36 publications

References 0 publications

Prolactin Release in the Sheep

Prolactin Release in the Sheep

Human Prolactin: Measurement in Plasma by In Vitro Bioassay

Radioimmunoassay for Human Pituitary Prolactin, Using Antiserum against an Extract of Human Amniotic Fluid

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