Radioimmunoassay for Human Pituitary Prolactin, Using Antiserum against an Extract of Human Amniotic Fluid

Cole, E.N.; Boyns, A. R.

doi:10.1159/000178313

Cited by 17 publications

(5 citation statements)

References 13 publications

(21 reference statements)

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“…Serum was separated immediately and stored at -20°C until assayed. Serum prolactin was measured using a double antibody technique (Cole and Boyns, 1973). The standard used was the MRC standard 71/222 containing 10 milliunits of prolactin per ampoule.…”

Section: Subjectsmentioning

confidence: 99%

Serum prolactin in liver disease and its relationship to gynaecomastia.

Morgan¹,

Jakobovits²,

Gore³

et al. 1978

Gut

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SUMMARY Serum immunoreactive prolactin was measured in 150 patients with liver disease of varying aetiology and severity and in 45 control subjects. The upper limit of the reference range for serum prolactin was 331 mU/1. Eighteen patients with liver disease (12%) had unexplained hyperprolactinaemia. No relationship existed between the prolactin value and the sex of the patient, the aetiology of the liver disease, the severity of the liver disease, or the presence of gynaecomastia. The cause of the hyperprolactinaemia in patients with liver disease and its clinical implications need further investigation.Feminisation can occur in men with chronic liver disease and also in male alcoholics with only minimal liver damage (

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Section: Subjectsmentioning

confidence: 99%

Serum prolactin in liver disease and its relationship to gynaecomastia.

Morgan¹,

Jakobovits²,

Gore³

et al. 1978

Gut

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“…Plasma uric acid was measured by a modification of the automated phosphotungstate colorimetric method (Nishi, 1967). Plasma prolactin was measured by radioimmunoassay (Cole & Boyns, 1973).…”

Section: Methodsmentioning

confidence: 99%

The Influence of Sodium and Potassium Supplements on the Diuretic Responses to Frusemide Administration in Normal Subjects

Branch

Cole

Horth³

et al. 1978

British J Pharmacology

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Twelve normal subjects received (1) normal diet, (2) normal diet with 100 mmol supplementary sodium chloride and (3) normal diet with 96 mmol supplementary potassium chloride, each for 10 days, in a balanced cross‐over study according to a Latin Square design. At the end of each study period, the subjects received 80 mg frusemide orally. Each study period was separated from the other by 10 days. Changes in urinary electrolyte excretion occurred within the first four days of each dietary period then remained constant, with significant differences in urinary Na/K ratio between the dietary regimes. Between‐subject correlations, using the mean values over the three study periods, demonstrated significant associations between plasma uric acid and urinary Na/K ratio and between plasma prolactin and urinary potassium excretion. Urinary potassium excretion and Na/K ratio following frusemide were influenced significantly by alteration of diet but there was no change in sodium excretion. Between‐subject correlations of pretreatment variables with diuretic response, using the mean values over the three study periods, demonstrated significant associations between both pretreatment urinary Na/K ratio and plasma uric acid and respectively the urinary potassium excretion and urinary Na/K ratio in response to frusemide. While the response to frusemide was altered by short‐term changes in dietary sodium and potassium, the difference was less than expected from observations in two populations with customary diets differing in similar manner.

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“…Plasma GH was estimated by a specific double antibody radioimmunoassay (Schalch and Parker, 1964) using purified human GH (MRC 69/46) and rabbit antiserum to the hormone (Wellcome Reagents Ltd, England). Plasma prolactin was measured by a homologous double antibody radioimmunoassay in which there was a negligible crossreaction with FSH, LH, TSH, GH and chorionic somatomammotrophin (Cole and Boyns, 1973). The sensitivities of the assay procedures for testosterone, oestradiol-178, LH, FSH, GH and prolactin were 0.052 nmol/l, 11 pmol/l 0.5 IU/I of MRC 63/15, 0.5 IU/I of 2nd-IRP-HMG, 0.5 mU/I and 0.03 U/I respectively as assessed by the method of Kaiser and Specker (1956).…”

Section: Hormone Assaysmentioning

confidence: 99%

Evaluation of Plasma Hormone Concentrations in Relation to Clinical Staging in Patients with Prostatic Cancer:BRITISH PROSTATE STUDY GROUP*

1979

British Journal of Urology

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Summary— Plasma concentrations of testosterone, oestradiol‐1 7β, luteinising hormone (LH), follicle stimulating hormone (FSH), prolactin and growth hormone (GH) were measured in patients with histologically proven prostatic cancer, before any form of therapy was given for this disease. Patients were categorised according to UICC classification. No systematic change in the group means of any of these hormones was associated with the progression of the disease from the TO to the T4 stage. When multivariate analysis was applied to the combined intraprostatic (TO + T1 + T2) and extraprostatic (T3 + T4) tumour category in patients without clinically evident metastases (MO) a discrimination was observed, GH substantially contributing to the separation of the 2 groups. When plasma hormone data from patients classified as MO (without metastases) were compared with M1 patients (with metastases), mean GH values were significantly larger (P<0.02) in patients with metastases. GH was also a major contributory factor to the discrimination between the MO and M1 groups, using multivariate analysis. Testosterone group means for MO versus M1 were also significantly different (P<0.02).

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Radioimmunoassay for Human Pituitary Prolactin, Using Antiserum against an Extract of Human Amniotic Fluid

Cited by 17 publications

References 13 publications

Serum prolactin in liver disease and its relationship to gynaecomastia.

Serum prolactin in liver disease and its relationship to gynaecomastia.

The Influence of Sodium and Potassium Supplements on the Diuretic Responses to Frusemide Administration in Normal Subjects

Evaluation of Plasma Hormone Concentrations in Relation to Clinical Staging in Patients with Prostatic Cancer:BRITISH PROSTATE STUDY GROUP*

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