A DNA-probe for the detection of the species Staphylococcus haemolyticus

Abstract

A 1.3-kb DNA fragment isolated from Staphylococcus haemolyticus strain DSM 20264 can be used as a specific probe for this species. The probe hybridized with 39 clinical isolates of S. haemolyticus but not with any of the 121 isolates representative of the other 25 species of staphylococci described to date.

The value of rRNA gene restriction site polymorphism analysis for delineating taxa in the genus Staphylococcus.

“…These approaches fall into two categories : those based on the detection of species-specific sequences that are not present in the chromosome of all staphylococci (Akatova et al, 1992 ;Chesneau et al, 1993a ;Martineau et al, 1996 ;Vandenesch et al, 1995) and those based on the detection of sequence variations in ubiquitous elements such as rRNA and tRNA operons (Bialkowska-Hobrzanska et al, 1990 ;Chesneau et al, 1992 ;De Buyser et al, 1989Forsman et al, 1997 ;Gaszewska-Mastalarz et al, 1998 ;Hesselbarth & Schwarz, 1995 ;Irlinger et al, 1997 ;Kluytmans et al, 1998 ;Maes et al, 1997 ;Mendoza et al, 1998 ;Pennington et al, 1991 ;Thomson-Carter et al, 1989 ;Webster et al, 1994 ;Welsh & McClelland, 1992) or chaperonin-encoding genes (Goh et al, 1997). The second of these categories of methods may be useful for classifying staphylococci if appropriate databases exist and are updated continuously as data for new taxa become available.…”

Isolation and characterization of a DNA probe forStaphylococcus aureussubspeciesaureusbiovar

“…Chromosomal DNA from NBSA M280(0) was prepared as previously described by Akatova et al [5]. DNA was digested with Hin dIII, ligated using T 4 DNA ligase with Hin dIII‐cleaved and phosphatase‐treated (calf intestinal alkaline phosphatase) vector pUC19 DNA [8].…”

“…Although helpful, biochemical identification has drawbacks, primarily relating to phenotypic alterations which may occur after repeat culturing of the isolates, or due to the physiological state of the culture [3, 4]. Genetic analysis, including DNA/DNA hybridization, remains the reference system for species identification [5, 6]. The aim of the present study was to identify a DNA fragment to be used as a gene probe for identifying these organisms [7].…”

Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar

“…Chromosomal DNA from NBSA M280(0) was prepared as previously described by Akatova et al [5]. DNA was digested with HindIII, ligated using T R DNA ligase with HindIII-cleaved and phosphatasetreated (calf intestinal alkaline phosphatase) vector pUC19 DNA [8].…”

“…Although helpful, biochemical identi¢cation has drawbacks, primarily relating to phenotypic alterations which may occur after repeat culturing of the isolates, or due to the physiological state of the culture [3,4]. Genetic analysis, including DNA/ DNA hybridization, remains the reference system for species identi¢cation [5,6]. The aim of the present study was to identify a DNA fragment to be used as a gene probe for identifying these organisms [7].…”