A disposable single-use electrochemical sensor for the detection of uric acid in human whole blood

Chen, J.-C.; Chung, Hsieh-Hsun; Hsu, Chao-Chun; Tsai, Dong‐Mung; Kumar, Arun; Zen, Jyh‐Myng

doi:10.1016/j.snb.2005.02.026

Cited by 110 publications

(46 citation statements)

References 23 publications

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“…[1][2][3] Nanobiosensors are used for sensing targets at a nanoscale, i.e., within 1-100 nm, and are capable of detecting biological events such as macromolecular-level alterations (metabolic and genetic) and sub-cellular-level infections (viral and bacterial). The detection signals generated are then converted into measurable patterns.…”

Section: Introductionmentioning

confidence: 99%

Nanomechanical Cantilever-Based Sensor: An Efficient Tool to Measure the Binding Between the Herbicide Mesotrione and 4-Hydroxyphenylpyruvate Dioxygenase

Rodrigues

Ierich

Andrade

et al. 2017

NANO

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Nanomechanical biosensors based on atomic force microscopy (AFM) cantilevers have garnered considerable attention. AFM cantilevers are devices that can detect a target either via a surface functionalization process based on immobilization through molecular adsorption, or through the selective chemical binding of a speci¯c molecule, transforming the device into a speci¯c biosensor. In this study, we demonstrate that functionalized AFM cantilevers could be used, in a process involving self-assembling layers, to create a homogeneous surface layer of the widely used herbicide mesotrione. Controlled experiments to evaluate its detection were performed, and binding between mesotrione and its target molecule, 4-hydroxyphenylpyruvate dioxygenase (HPPD), was evaluated using de°ection curves of functionalized cantilevers interacting with mesotrione. The cantilevers worked as nanomechanical sensors inside a°uid cell device, under di®erent ¶ Corresponding author. 1750079-1NANO: Brief Reports and Reviews Vol. 12, No. 7 (2017) concentrations of HPPD diluted in PBS. After evaluating increasing concentrations of HPPD, the de°ection curves showed a clear, dose-dependent pattern. The homogeneous dispersion of mesotrione on the cantilevers was assessed by confocal microscopy, and this corroborated the functionalization method. Thus, the results obtained by this functionalized cantilever presented a high e±ciency in detecting binding between HPPD and mesotrione molecules at concentrations as low as 17 ng mL À1 . In this way, as a preliminary step for a future environmental contaminants nanosensor development, the described detection method showed a suitable capability for molecular recognition at the nanoscale.

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Section: Introductionmentioning

confidence: 99%

Nanomechanical Cantilever-Based Sensor: An Efficient Tool to Measure the Binding Between the Herbicide Mesotrione and 4-Hydroxyphenylpyruvate Dioxygenase

Rodrigues

Ierich

Andrade

et al. 2017

NANO

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“…22 After adequate preoxidation, the SPCEs can present more oxygen-containing functionality and edge-plane sites, which makes the SPCE surface hydrophilic, and reduces the redox overpotential of various analytes. [23][24][25][26] Therefore, it is worth noting the effect of different preoxidizing procedures of SPCEs on the roughness of AuNS electrodeposition and the corresponding impedimetric properties of immunosensors. Moreover, the sensing characteristics of the impedimetric immunesensors are not only determined by the electrode roughness, but also by the physical properties of the modification layer.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Chain Length of a Modified Layer and Surface Roughness of an Electrode on Impedimetric Immunosensors

Lin

2017

ANAL. SCI.

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An ultrasensitive label-free impedimetric immunosensor is constructed by modifying a 3-mercaptoproponic acid (MPA) monolayer on highly rough gold nanostructure (AuNS)-electrodeposited screen printed carbon electrodes (SPCEs) for the detection of small molecular weight drugs (SMWDs), such as salbutamol (SAL). The SPCEs preoxidized in a 0.1 M H2SO4 solution (called po-SPCEH2SO4) are electrodeposited with the AuNS to increase the roughness factor to 23.64 ± 1.76, larger than the AuNS/po-SPCENaOH or the AuNS/po-SPCEPBS. Furthermore, the MPA modified layer as a link for the anti-SAL immobilization to give the immunosensors an impedimetric signal-to-noise ratio larger than the 11-mercaptoundecanoic acid-and 16-mercaptohexadecanoic acid-modified layer, due to the lower interfacial impedance of the MPA monolayer. The MPA/AuNS/po-SPCEH2SO4-based immunosensors have a wide linear range of 1 fg mL -1 to 1 ng mL -1 and a limit of detection of 0.6 fg mL -1 . Moreover, the immunosensors can practically quantify the SAL concentrations in 1000 times-diluted serum samples with good recovery.

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“…3 Uric acid levels are very high levels can cause kidney damage, liver damage and cardiovascular disease. 4 Controlling uric acid levels should be done early in order to do such a dangerous disease prevention. In general, the methods used for the analysis of uric acid levels are colorimetric with chemical reagents or by enzymatic reactions.…”

Section: Introductionmentioning

confidence: 99%

“…In general, the methods used for the analysis of uric acid levels are colorimetric with chemical reagents or by enzymatic reactions. Chen et al (2005) 4 analyzed the levels of uric acid in the blood by spectrophotometry using fosfotungstat acid reagent or enzyme uricase (uric oxidase). The analysis method of uric acid by spectrophotometry requires approximately 2-3 mL blood sample, have low sensitivity, and the detection limit is relatively high (mM).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Uric Acid Using Carbon Paste Electrodes Modified by Molecularly Imprinted Polymer as Potentiometry Sensor

Darmokoesoemo¹,

Widayanti²,

Khasanah³

et al. 2017

Rasayan J Chem

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The development of carbon paste electrodes modified with the molecularly imprinted polymer (MIP) for the potentiometric analysis of uric acid was carried out in this study. MIP was made from methyl methacrylate as a monomer, ethylene glycol dimethacrylate as crosslinker, and uric acid as a template. The carbon paste electrodes/MIP is produced from carbon, MIP, and paraffin with ratio 40 : 25 : 35 (%, w/w). The measurement of uric acid with carbon paste electrodes/MIP produced Nernst factor of 30.19 mV/decade with a measurement range of 10 -6 -10 -3 M. The minimum detection limit of this method was 3.03.10 -6 M, the precision and accuracy towards uric acid with a concentration of 10 -6 -10 -3 M was ranging between 1.36-2.03% and 63.9-166%. The selectivity coefficient value was less than one which indicated that the electrode was selective against uric acid and was not interfered by urea. This electrode has a response time less than two minutes and lifetime is eight weeks with the usage is 104 times.

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A disposable single-use electrochemical sensor for the detection of uric acid in human whole blood

Cited by 110 publications

References 23 publications

Nanomechanical Cantilever-Based Sensor: An Efficient Tool to Measure the Binding Between the Herbicide Mesotrione and 4-Hydroxyphenylpyruvate Dioxygenase

Nanomechanical Cantilever-Based Sensor: An Efficient Tool to Measure the Binding Between the Herbicide Mesotrione and 4-Hydroxyphenylpyruvate Dioxygenase

Effect of the Chain Length of a Modified Layer and Surface Roughness of an Electrode on Impedimetric Immunosensors

Analysis of Uric Acid Using Carbon Paste Electrodes Modified by Molecularly Imprinted Polymer as Potentiometry Sensor

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