A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen

Wu, Jie; Tang, Jinhai; Dai, Zong; Yan, Feng; Ju, Huangxian; Murr, Nabil El

doi:10.1016/j.bios.2005.12.008

Cited by 173 publications

(65 citation statements)

References 46 publications

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“…All working electrodes shared the same reference and auxiliary electrodes. We fabricated the 10-electrode array according to a previous report (36 ). The insulating layer printed around the working area constituted a reservoir of the electrochemical microcell.…”

Section: Fabrication Of Immunosensor Arraymentioning

confidence: 99%

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

et al. 2007

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Background: Automated and convenient multianalyte detection with high throughput is increasingly needed in clinical diagnosis. We developed a disposable 4-by-2 array for programmed simultaneous amperometric immunoassay of 4 tumor markers. Methods: We used a screen-printed technique, 1-step immobilization method, and flow injection technique. We immobilized carcinoembryonic antigen, ␣-fetoprotein, ␤-human choriogonadotropin, and carcinoma antigen 125 as model analytes in a redox mediator-grafted, biopolymer-modified, screen-printed carbon electrode array to capture corresponding horseradish peroxidaselabeled antibodies in competitive immunoreactions. The simultaneous multianalyte immunoassay was automatically carried out to amperometrically monitor the mediator-catalyzed enzymatic response to hydrogen peroxide, which decreased in proportion to the concentrations of analytes in samples. Results: The multianalyte immunosensor array had a throughput of 60 samples/h and allowed simultaneous detection of carcinoembryonic antigen, ␣-fetoprotein, ␤-human choriogonadotropin, and carcinoma antigen 125 in clinical serum samples with concentrations up to 188 g/L, 250 g/L, 266 IU/L, and 334 kIU/L, respectively. The detection limits (limits of the blank, mean of blank plus 3 SD) were 1.1 g/L, 1.7 g/L, 1.2 IU/L, and 1.7 kIU/L. The inter-and intraassay imprecision (CVs) of the immunosensor arrays were <7.8% and <9.0%, respectively. The immunosensor arrays were stable for 28 days.

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Section: Fabrication Of Immunosensor Arraymentioning

confidence: 99%

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

et al. 2007

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“…1. The 6-electrode array was fabricated as reported (32 ), and it contained 4 graphite working electrodes (2 mm in diameter, 1 mm edge-to-edge separation), 1 graphite auxiliary electrode, and 1 Ag/AgCl reference electrode. All working electrodes shared the same reference and auxiliary electrodes.…”

Section: Fabrication Of Immunosensors Arraymentioning

confidence: 99%

Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

Yan

Zhang

et al. 2008

Clinical Chemistry

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“…ELISA was commonly used with good selectivity and high sensitivity, but time-consuming and complex operations. An electrochemical immunosensor for 0.5-25 ng/mL CEA was reported with DL of 0.22 ng/mL, 19 but immunosensor had a lower responsive velocity when the CEA concentration was lower.…”

Section: Introductionmentioning

confidence: 98%

“…In clinical laboratory, the periodical detection of CEA can be as an index for prognosis estimation, recrudescence and transfer prediction, and clinical treatment effect. [1][2][3][4][5][6][7] At present, several methods such as radioimmunoassay (RIA), [8][9][10][11] chemiluminescent immunoassay (CLIA), [12][13][14][15] time-resolved fluoroimmunoassay (TR-FIA), 16,17 enzyme-linking immunoassay (ELISA), 18 and electrochemical immunoassay (ECIA), 19,20 have been reported for assay of CEA. REIA has been commonly used to detect CEA, and 05 mg/mL CEA could be detected by RIA using 125 I label, but this method is time-comsuming and radioactive.…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Highly Selective Resonance Scattering Spectral Assay for Trace Carcinoembryonic Antigen

Jiang

Deng

2008

Chin. J. Chem.

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Gold nanoparticles, in size of 10 nm, were used to label monoclonal carcinoembryonic antigen antibody (CEAAb) to obtain an immunonanogold resonance scattering (RS) spectral probe (AuCEAAb) for the measurement of trace carcinoembryonic antigen (CEA). In pH 6.8 Na 2 HPO 4 -NaH 2 PO 4 buffer solution and the presence of polyethylene glycol (PEG)-6000, the AuCEAAb combined with CEA to form the nanogold-labeled immunocomplex clusters in average size of 227 nm, which exhibited two scattering peaks at about 320 and 581 nm. The enhanced RS intensity at 581 nm (∆I 581 nm ) is proportional to CEA concentration in the range of 1.0-50.0 ng/mL, with a detection limit (DL) of 0.52 ng/mL. The immunonanogold RS assay was utilized to determine CEA in serum samples with high sensitivity, good selectivity and simplicity.

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A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen

Cited by 173 publications

References 46 publications

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

A Rapid and Highly Selective Resonance Scattering Spectral Assay for Trace Carcinoembryonic Antigen

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