1990
DOI: 10.1128/mcb.10.3.972-981.1990
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A Directly Repeated Sequence in the β-Globin Promoter Regulates Transcription in Murine Erythroleukemia Cells

Abstract: We have identified a previously undetected cis-acting element in the mouse beta-major globin promoter region that is necessary for maximal transcription levels of the gene in the inducible preerythroid murine erythroleukemia (MEL) cell line. This element, termed the beta-globin direct-repeat element (beta DRE), consists of a directly repeated 10-base-pair sequence, 5'-AGGGCAG(G)AGC-3', that lies just upstream from the TATA box of the promoter. The beta DRE motif is highly conserved in all adult mammalian beta-… Show more

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Cited by 3 publications
(4 citation statements)
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“…5). The proximal GATA-1 (pPG60M) and CACC (p,CCM) promoter mutants are comparatively inactive, confirming and extending previous results (6,11,32,44). In contrast, the distal NF1 (pPNFlM), GATA-1 (pPi215M), and BB1 (pPBBlM) promoter mutants are fourto eightfold more active than ppWT.…”
Section: Resultssupporting
confidence: 87%
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“…5). The proximal GATA-1 (pPG60M) and CACC (p,CCM) promoter mutants are comparatively inactive, confirming and extending previous results (6,11,32,44). In contrast, the distal NF1 (pPNFlM), GATA-1 (pPi215M), and BB1 (pPBBlM) promoter mutants are fourto eightfold more active than ppWT.…”
Section: Resultssupporting
confidence: 87%
“…The nuclear DHS of the mouse ,-major-globin gene promoter in MEL cells spans a 250-bp (-1 to -250 bp) sequence (4). A GATA-1 protein binding site at -212 bp (38) and conserved motifs such as the CCAAT, CACC, ,BDRE, and TATA motifs, which were identified in functional assays (6,11,32,44), have been described. Using in vitro protein binding assays and competitor oligonucleotides (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…While Pol II is a binding partner of TFIIB in the pre-initiation complex, this ChIP does have the limitation of indirectly immunoprecipitating other factors in the transcriptional pre-initiation complex and might also reflect general promoter activity. The β-DRF motif has been shown to be important for high transcription of HBB , but its bona fide binding factor has not yet been identified Stuve and Myers, 1990 . In the homozygous clones K and KDT, which harbor the edited KLF1 sequence, we observed increased binding of KLF1 to the HBD promoter relative to unedited WT HUDEP-2 cells.…”
Section: Resultsmentioning
confidence: 99%