1993
DOI: 10.1128/mcb.13.2.1093-1103.1993
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Erythroid Differentiation of Mouse Erythroleukemia Cells Results in Reorganization of Protein-DNA Complexes in the Mouse βMaj Globin Promoter but not its Distal Enhancer

Abstract: Dimethyl sulfoxide (DMSO) induction of mouse erythroleukemia (MEL) cells represents a well-defined in vitro system of terminal erythroid differentiation. We have studied the molecular mechanisms of transcriptional activation of the mouse beta maj globin gene during MEL cell differentiation by analyzing nuclear factor-DNA interactions in vivo at the gene's upstream promoter and a distal enhancer, 5'HS-2. Genomic footprinting data indicate that three motifs, CAC, NF-E2/AP1, and GATA-1, of the 5'HS-2 enhancer are… Show more

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Cited by 6 publications
(1 citation statement)
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“…To specifically knockdown miR-125b-5p in erythroblasts, BALB/c mice were transduced with adeno-associated virus (AAV) serotype 6 which carried beta globin enhancer (GenBank: S73747.1) and promoter (GenBank: GU057255.1) [ 47 ] driving enhanced green fluorescent protein (eGFP) together with either miR-125b sponge (8-repeat antisense-miR-125b-5p) or control (8-repeat scramble nucleotides). The designed enhancer-promoter-eGFP with 8 tandem repeat miR-125b-5p sponges (5′-TCACAAGTTAGGGTCTCAGGGA-3′) or a control (5′-AAGTTTTCAGAAAGCTAACA-3′) [ 48 ] was cloned into AAV by Genechem Co. Ltd. (Shanghai, China).…”
Section: Methodsmentioning
confidence: 99%
“…To specifically knockdown miR-125b-5p in erythroblasts, BALB/c mice were transduced with adeno-associated virus (AAV) serotype 6 which carried beta globin enhancer (GenBank: S73747.1) and promoter (GenBank: GU057255.1) [ 47 ] driving enhanced green fluorescent protein (eGFP) together with either miR-125b sponge (8-repeat antisense-miR-125b-5p) or control (8-repeat scramble nucleotides). The designed enhancer-promoter-eGFP with 8 tandem repeat miR-125b-5p sponges (5′-TCACAAGTTAGGGTCTCAGGGA-3′) or a control (5′-AAGTTTTCAGAAAGCTAACA-3′) [ 48 ] was cloned into AAV by Genechem Co. Ltd. (Shanghai, China).…”
Section: Methodsmentioning
confidence: 99%