A dielectrophoresis method to manipulate and monitor theShewanella oneidensis

Abstract

Shewanella oneidensis is capable of producing electrons and transferring to the solid electrodes. To characterize the electrical properties of S. oneidensis and manufacture the miniature microbial fuel cells, cellular dielectrophoresis has been developed into a powerful and flexible tool to utilize microfabricated electrode arrays fulfilling S. oneidensis manipulation and monitoring. When S. oneidensis with green fluorescent protein were placed under the influence of an applied inhomogeneous field, the translation and enrichment of the green bacteria were observed by the fluorescence microscope. The distribution of electric field around the microelectrodes was simulated in finite element analysis software. The dielectrophoresis manipulation parameters were also optimized according to the peak value of applied alternating current signals and its frequency. In short, this study shows that dielectrophoresis can be a valuable tool to manipulate S. oneidensis to the sidewalls of microelectrode.

Observation Interface of PDMS Membrane in a Microfluidic Chip Based on One-Step Molding

“…The glass substrate with a microelectrode layer and a microvoid array layer was fabricated using ultraviolet photolithography. The fabrication process of the glass substrate was similar to our previous work [ 16 ]. Subsequently, the PDMS replica was fabricated according to the one-step molding method.…”

Dielectrophoresis: From Molecular to Micrometer-Scale Analytes

“…Narayanan et al reported separation of C. muris and G. lambia using indium tin oxide (ITO) electrodes in a PDMS device. Chen et al , recently reported DEP devices for trapping and counting S. oneidensis cells. The device consisted of an array for electrodes and holes to trap bacterial cells (expressing green fluorescent protein), and the fluorescence signal from the trapped cells was used for quantification.…”