Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level

Chen, Xiangyu; Liang, Zhiting; Li, Daobo; Xiong, Ying; Xiong, Penghui; Guan, Yong; Hou, Shuangyue; Hu, Yue; Chen, Shan; Liu, Gang; Tian, Yangchao

doi:10.1016/j.bios.2017.08.017

Cited by 43 publications

(31 citation statements)

References 35 publications

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“…Moving from yeast to bacteria, Chen et al. fabricated a microfluidic DEP platform that was capable of trapping, counting, and detecting Shewanella oneidensis [67]. This device achieved rapid, label‐free detection of S. oneidensis using a castellated, or interdigitated, electrode configuration with a central flow channel.…”

Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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The 20th century has seen tremendous innovation of dielectrophoresis (DEP) technologies, with applications being developed in areas ranging from industrial processing to micro‐ and nanoscale biotechnology. From 2010 to present day, there have been 981 publications about DEP. Of over 2600 DEP patents held by the United States Patent and Trademark Office, 106 were filed in 2019 alone. This review focuses on DEP‐based technologies and application developments between 2010 and 2020, with an aim to highlight the progress and to identify potential areas for future research. A major trend over the last 10 years has been the use of DEP techniques for biological and clinical applications. It has been used in various forms on a diverse array of biologically derived molecules and particles to manipulate and study them including proteins, exosomes, bacteria, yeast, stem cells, cancer cells, and blood cells. DEP has also been used to manipulate nano‐ and micron‐sized particles in order to fabricate different structures. The next 10 years are likely to see the increase in DEP‐related patent applications begin to result in a greater level of technology commercialization. Also during this time, innovations in DEP technology will likely be leveraged to continue the existing trend to further biological and medical‐focused applications as well as applications in microfabrication. As a tool leveraged by engineering and imaginative scientific design, DEP offers unique capabilities to manipulate small particles in precise ways that can help solve problems and enable scientific inquiry that cannot be addressed using conventional methods.

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Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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“…Manipulation of model organism has been demonstrated by Chen et al [18], who perform detection and trapping of Shewanella oneidensis, a model organism for electrochemical activity bacteria, using DEP microfluidic chip, which equipped with real-time imaging for trapping process observation. The trapping is enhanced by providing hole arrays on top of the electrode, in which the hole size-bacteria count relation is also studied.…”

Section: Dielectrophoretic Manipulation Of Bioparticlesmentioning

confidence: 99%

Biological Particle Control and Separation using Active Forces in Microfluidic Environments

Ali¹,

Kayani²,

Majlis³

2018

Microfluidics and Nanofluidics

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Exploration of active manipulation of bioparticles has been impacted by the development of micro-/nanofluidic technologies, enabling evident observation of particle responses by means of applied tunable external force field, namely, dielectrophoresis (DEP), magnetophoresis (MAG), acoustophoresis (ACT), thermophoresis (THM), and optical tweezing or trapping (OPT). In this chapter, each mechanism is presented in brief yet concise, for broad range of readers, as strong foundation for amateur as well as brainstorming source for experts. The discussion covers the fundamental mechanism that underlying the phenomenon, presenting the theoretical and schematic description; how the response being tuned; and utmost practical, the understanding by specific implementation into bioparticles manipulation engaging from micron-sized material down to molecular level particles.

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“…Although this is an effective analysis tool, it is highly manual, has low throughput, and is not easily automated. There is also a number of approaches that use DEP trapping to infer dielectric properties [27,41–43]. There are DEP trapping‐based systems to measure the dielectrophoretic collection rate [9,40].…”

Section: Introductionmentioning

confidence: 99%

Parallel single‐cell optical transit dielectrophoresis cytometer

et al. 2020

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In this work, we present an optical transit DEP flow cytometer for parallel single-cell analysis. Each cell's dielectric property is inferred from velocity perturbations due to DEP actuation in a microfluidic channel. Dual LED sources facilitate velocity measurement by producing two transit shadows for each cell passing through the channel. These shadows are detected using a 256-pixel linear optical array detector. Massively parallel analysis is possible as each pixel of the detector can independently analyze the passing cells. A wide channel (∼18 mm) was employed to carry many particles simultaneously, and the system was capable of detecting the velocity of over 200 cells simultaneously. We have achieved analysis rates for 10 µm diameter polystyrene spheres response exceeding 250 per second. With appropriate calibration, this DEP cytometer can quantitatively measure the dielectric response. The dielectric response (Clausius-Mossotti factor) of viable CHO cells was measured over the frequency range of 100 kHz to 6 MHz, and the obtained response matches the previously measured values by our group. The DEP cytometer uses simple modular components to achieve high throughput label-free single-cell dielectric analysis and can begin analyzing particles within 10 s after starting to pump the sample into the channel.

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Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level

Cited by 43 publications

References 35 publications

Dielectrophoresis: Developments and applications from 2010 to 2020

Dielectrophoresis: Developments and applications from 2010 to 2020

Biological Particle Control and Separation using Active Forces in Microfluidic Environments

Parallel single‐cell optical transit dielectrophoresis cytometer

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