A degradation fragment of type X collagen is a real-time marker for bone growth velocity

Coghlan, Ryan; Oberdorf, Jon; Sienko, Susan; Aiona, Michael D.; Boston, Bruce A.; Connelly, Kara J.; Bahney, Chelsea S.; Larouche, Jérémie; Almubarak, Sarah; Coleman, Daniel T.; Girkontaitė, Irutė; Mark, Klaus von der; Lunstrum, Gregory P.; Horton, William A.

doi:10.1126/scitranslmed.aan4669

Cited by 46 publications

(74 citation statements)

References 40 publications

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“…Most interestingly, a recently published study has shown that a group of fragments containing the entire C1q domain and variable portions of the attached linker and collagenous region designated as CXM, was a degradation product of type X collagen during endochondral ossification and an ideal biomarker for the overall growth plate activity 32 . The serum-based assay for CXM used in this study was built upon a mAb X34 specifically targeting a multimeric form of the NC1 domain, the~50 kDa NC1 region 10 .…”

Section: Discussionmentioning

confidence: 99%

Potential diagnostic value of a type X collagen neo-epitope biomarker for knee osteoarthritis

Manon‐Jensen

Arendt-Nielsen

et al. 2019

Osteoarthritis and Cartilage

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s u m m a r yObjective: Phenotypic changes of chondrocytes toward hypertrophy might be fundamental in the pathogenesis of osteoarthritis (OA), of which type X collagen (Col10) is a well-known marker. The purpose was to develop a specific immunoassay for blood quantification of a newly identified neo-epitope of type Col10 to assess its diagnostic value for radiographic knee OA. Methods: A neo-epitope of Col10 was identified in urine samples from OA patients. A monoclonal antibody against the neo-epitope was produced in Balb/C mice. The enzyme responsible for the cleavage was identified. Immunohistochemical detection of this neo-epitope was performed on human OA cartilage. An immunoassay (Col10neo) was developed and quantified in two clinical studies: the C4Pain-003 and the NYU OA progression study. Receiver operating characteristic curve (ROC) curve analysis was carried out to evaluate the discriminative power of Col10neo between OA and rheumatoid arthritis (RA). Results: A neo-epitope specific mAb was produced. The Cathepsin K-generated neo-epitope was localized to the pericellular matrix of chondrocytes, while its presence was extended and more prominent in superficial fibrillation in the cartilage with advanced degradation. In the C4Pain study, a higher level of Col10neo was seen in subjects with greater KL grade. The group of the highest tertile of Col10neo included more subjects with KL3-4. In the NYU study, Col10neo was statistically higher in OA than control or RA. ROC curve analysis revealed area under the curve was 0.88 (95% CI 0.81e0.94). Conclusion: Our findings indicate that Col10neo linked to hypertrophic chondrocytes could be used as a diagnostic biochemical marker for knee OA.

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Section: Discussionmentioning

confidence: 99%

Potential diagnostic value of a type X collagen neo-epitope biomarker for knee osteoarthritis

Manon‐Jensen

Arendt-Nielsen

et al. 2019

Osteoarthritis and Cartilage

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“…Few studies have addressed the association between asymptomatic viral infections and growth . To better assess the growth impact of non‐chronic viral infections, one should thus probably use with a short follow‐up period a more sensitive outcome variable, such as knemometry, plasma concentrations of insulin‐like growth factor or collagen X .…”

Section: Discussionmentioning

confidence: 99%

Presence of Giardia lamblia in stools of six‐ to 18‐month old asymptomatic Malawians is associated with children's growth failure

et al. 2019

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Aim Despite high pathogen burden and malnutrition in low‐income settings, knowledge on relationship between asymptomatic viral or parasitic infections, nutrition and growth is insufficient. We studied these relationships in a cohort of six‐month‐old Malawian infants. Methods As part of a nutrient supplementation trial for 12 months, we documented disease symptoms of 840 participant daily and anthropometric measurements every three months. Stool specimens were collected every six months and analysed for Giardia lamblia, Cryptosporidium species and enterovirus, rotavirus, norovirus, parechovirus and rhinovirus using polymerase chain reaction (PCR). The prevalence of the microbes was compared to the children's linear growth and the dietary. Results The prevalence of the microbes was similar in every intervention group. All age groups combined, children negative for G. lamblia had a mean standard deviation (SD) of −0.01 (0.49) change in length‐for‐age Z‐score (LAZ), compared to −0.12 (0.045) among G. lamblia positive children (difference −0.10, 95% CI −0.21 to −0.00, p = 0.047). The LAZ change difference was also statistically significant (p = 0.042) at age of 18–21 months but not at the other time points. Conclusion Asymptomatic G. lamblia infection was mainly associated with growth reduction in certain three‐month periods. The result refers to the chronic nature of G. lamblia infection.

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“…Furthermore, COLX involved in hematopoiesis, mineralization, and endochondral ossification. The COLX is stored in the hypertrophic zone and is removed during the endochondral ossification process (Chang et al 2012;Gu et al 2014;Coghlan et al 2017). Type X-collagen production increases progressively during the development of the embryonic framework, thereby accelerating embryo growth (Prickett 2014).…”

Section: Resultsmentioning

confidence: 99%

Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia

Susanti

Yuniastuti

2020

Biodiversitas

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Abstract. Susanti R, Yuniastuti A. 2020. Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia. Biodiversitas 21: 605-610. Genetic conservation is an effective approach to preserve unique and protected species in Indonesia, including indigenous duck (Anas platyrhynchos Linnaeus, 1758). One of the most important things is an identification effort by studying genetic characteristics to support genetic resource conservation programs. This study aim was to analyze the genetic polymorphisms of Central Javanese local duck based on prolactin (PRL) and collagen-x (COLX) genes. A total of 35 local ducks in Central Java was collected as samples, the duck’s calamus feathers were taken for tissue DNA isolation. Polymorphism analysis of PRL and COLX genes were performed using single nucleotide polymorphism (SNP) markers and PCR-RFLP method. The amplicons were then digested with DraI restriction enzyme (AhaIII) (5'TTT/ AAA) for PRL gene, and HaeIII (BsuRI) enzyme (5'GG/ CC-3 ') for COLX gene. The genotype was determined based on RFLP PCR amplicon band results from each target gene. For each gene, data on genotype frequencies, allele frequencies, expected heterozygosities (HE), and Hardy-Weinberg equilibrium were analyzed using PopGene32 software. Based on the PRL gene, there were 25 ducks (71.43%) with BB genotype and 10 ducks (28.57%) with AB genotype. While based on the COLX gene, as many as 25 ducks (71.43%) had TT genotypes, 9 ducks (25.71%) had TC types and 1 duck (1.86%) had CC types. Both PRL and COLX genotypes were clustered the Central Javanese local ducks into 5 haplotypes (A-E). The high allele frequency has belonged to B allele (85.71%) of the PRL gene and T allele (84.25%) of the COLX gene that showed high egg production and quality. Allele B (PRL gene) and T allele (COLX gene) could be predicted as weight eggs’ alleles marker.

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A degradation fragment of type X collagen is a real-time marker for bone growth velocity

Cited by 46 publications

References 40 publications

Potential diagnostic value of a type X collagen neo-epitope biomarker for knee osteoarthritis

Potential diagnostic value of a type X collagen neo-epitope biomarker for knee osteoarthritis

Presence of Giardia lamblia in stools of six‐ to 18‐month old asymptomatic Malawians is associated with children's growth failure

Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia

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