A critical analysis of human immunodeficiency virus transmission using human cartilage allografts

Bujía, J; Pitzke, P.; Wilmes, E.; Hammer, C.; Gürtler, Lutz

doi:10.1007/bf00176950

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…The absence of any vascularization and the complex mucopolysaccharide matrix forms a barrier to blood and immune cells. The absence of the CD4 receptor on human cartilage tissue has recently been reported by our group, excluding a possible infection of cartilage cells by the CD4-dependent mechanisms [7].…”

Section: Introductionmentioning

confidence: 96%

“…When the first antibody (negative control) was omit ted, no background staining was observed. We investigated the presence of CD4 antigens in the chondrocyte cell surface and could demonstrate the ab sence of such antigens [7], The present study demon strates that human cartilage cells obtained from the nasal septum are not susceptible for productive infection with HIV. The chondrocytes were cultured with a viral inocu lum 10,000-fold higher than the minimal doses sufficient for the infection of a human being (in analogy to the find ing that 1 monkey-infectious dose of SIV corresponds to 100 TCID50 [22]).…”

Section: Immunocytochemical Staining Procedures Using Alkaline Phosphmentioning

confidence: 99%

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Human Immunodeficiency Virus Cannot Productively Infect Freshly Cultured Human Cartilage Cells

Bujía¹,

Meyer²,

Hammer³

et al. 1993

ORL

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The use of cartilage allografts is being discussed because of the possible transmission of the human immunodeficiency virus (HIV). To further delineate the possibility for an HIV infection of cartilage cells the susceptibility and permissivity of normal human chondrocytes to HIV-1 was assessed in culture. Isolated cartilage cells were incubated during 30 days with the HIV, testing the production of viral p24 antigens and the formation of particle-bound reverse transcriptase (RT) in the supernatant. The H9 cell line was used as positive control. The production of p24 antigen did not occur on chondrocytes during the whole culture time. Furthermore, no RT activity was detected throughout the entire experimental period. Our results indicate clearly that HIV was not able to infect cartilage cells under these in vitro conditions, and, conclusively, HIV infection of cartilage in vivo is improbable.

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Section: Introductionmentioning

confidence: 96%

Section: Immunocytochemical Staining Procedures Using Alkaline Phosphmentioning

confidence: 99%

Human Immunodeficiency Virus Cannot Productively Infect Freshly Cultured Human Cartilage Cells

Bujía¹,

Meyer²,

Hammer³

et al. 1993

ORL

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“…Both CD4‐dependent (fusion with the plasma membrane through interaction with a membrane receptor) and CD4‐independent mechanisms of nonlym‐phoid‐cell infection (phagocytosis and endocytosis) have been suggested for the onset of infection 20 . To examine the possibility of HIV transmission using cartilage allografts, Bujía, et al 21 previously investigated the presence of CD4 antigens on the chondrocyte cell surface and demonstrated the absence of such antigens. Furthermore, it was shown that human cartilage cells obtained from nasal septum are not susceptible to productive infection with HIV when these cells are cocultivated with HIV in tissue culture 22 .…”

Section: Discussionmentioning

confidence: 99%

Influence of Chemical Allograft Preservation Procedures on the Human Immunodeficiency Virus

et al. 1996

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Since chemically preserved allogenic transplants have an established place in reconstructive procedures, the possibility of transferring the human immunodeficiency virus (HIV) with these transplants has been intensively discussed. In this study the authors obtained brain and spleen samples from six HIV-infected cadavers and preserved them with Merthiolate, Cialit, and formaldehyde. After preservation, the tissues were examined for proviral HIV-1 DNA (gag, pol, env) using the polymerase chain reaction. Proviral sequences were clearly demonstrated after the preservation procedure. The results of this study indicate that HIV remains in tissues that have been treated with Merthiolate, formaldehyde, or Cialit. Further investigations are necessary to determine if the virus is in an inactivated or activated form. It can be concluded that, because of the possible transmission of HIV by chemically preserved homografts, serologic screening of donors should be mandatory.

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“…Human chondrocytes lack a CD4 receptor and therefore are not considered to be susceptible to HIV infection through a CD4-dependent mechanism (Bujia et al 1993b). Other mechanisms leading to possible HIV infection of chondrocytes have been examined.…”

mentioning

confidence: 99%

Hiv Infection of Human Cartilage

Campbell

Oakeshott

1996

The Journal of Bone and Joint Surgery. British volume

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I nfection of human cartilage with HIV in vivo hasnot previously been reported. Specimens of articular cartilage taken at postmortem from ten patients who were HIV-positive were examined. Two had AIDS and eight were believed to have stage-2 disease.The standard polymerase chain reaction (PCR) protocol was modified to allow semiquantitative analysis of the samples. Oligonucleotide primers labelled with 32 P gamma-ATP were used to detect a segment of HIV DNA and a control DNA gene segment (HLA genome) to estimate the ratio of infected cells. The 32 P-labelled PCR products were separated on acrylamide gels and visualised directly by autoradiography and computer densitometry.Infection of human cartilage in vivo was demonstrated in nine of the ten samples in which the PCR analysis was positive. The other did not react sufficiently to produce detectable radiolabelled PCR product despite repeated DNA digestion and extraction. Cartilage infected with HIV could be a potential source of HIV when used in operations.

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A critical analysis of human immunodeficiency virus transmission using human cartilage allografts

Cited by 5 publications

References 24 publications

Human Immunodeficiency Virus Cannot Productively Infect Freshly Cultured Human Cartilage Cells

Human Immunodeficiency Virus Cannot Productively Infect Freshly Cultured Human Cartilage Cells

Influence of Chemical Allograft Preservation Procedures on the Human Immunodeficiency Virus

Hiv Infection of Human Cartilage

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