A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda

Sun, Yuying; Zhang, Jiquan; Xiang, Jianhai

doi:10.1016/j.fsi.2017.09.065

Cited by 27 publications

(8 citation statements)

References 28 publications

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“…563 Further research on delivery methods of CRISPR-Cas is required to achieve efficient in vivo genome editing in penaeid shrimp, for example via microinjection of CRISPR-Cas9, as has been successfully applied to the decapod (non-penaeid) shrimp Exopalaemon carinicuda. 555,[563][564][565][566][567][568] Based on results from large-scale and integrated genomic analyses, target genes could be chosen for the investigation of their functional role using genome editing tools. These targets could be candidate genes or pathways purported to underlie intra-or inter-specific genetic variation in host resistance to the pathogen, for example, candidate genes within a QTL region.…”

Section: Targeted Editing Of Candidate Loci In Embryos and Cellsmentioning

confidence: 99%

“…Genome-edited hosts, along with unedited and mock-edited control animals of the same families can be challenged with the pathogen of interest to assess whether the gene(s) subjected to editing affect host Knockout of chitinase resistance by measuring and comparing host survival and/or pathogen load, as has been done to explore the effect of the chitinase gene on ridge back white shrimp host resistance to Aeromonas hydrophila and Vibrio parahaemolyticus. 555 DNA and RNA samples would be collected to check if the edit is present in the target organ and if it has the desired effect on gene expression. Tests for mosaicism (using PCR and sequencing) and checks for obvious effects on the external phenotype and behaviour should also be carried out.…”

Section: Testing the Effects Of Edits On Host Resistancementioning

confidence: 99%

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Applying genetic technologies to combat infectious diseases in aquaculture

Robinson

Robledo

Sveen

et al. 2022

Reviews in Aquaculture

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Disease and parasitism cause major welfare, environmental and economic concerns for global aquaculture. In this review, we examine the status and potential of technologies that exploit genetic variation in host resistance to tackle this problem. We argue that there is an urgent need to improve understanding of the genetic mechanisms involved, leading to the development of tools that can be applied to boost host

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Section: Targeted Editing Of Candidate Loci In Embryos and Cellsmentioning

confidence: 99%

Section: Testing the Effects Of Edits On Host Resistancementioning

confidence: 99%

Applying genetic technologies to combat infectious diseases in aquaculture

Robinson

Robledo

Sveen

et al. 2022

Reviews in Aquaculture

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“…In Exopalaemon carinicauda, a wide range of indels at the E. carinicauda chitinase 4 (EcChi4) gene locus in the genome was induced by the CRISPR/Cas9 system using a microinjection method (Gui et al, 2016). EcChi4 knockout prawns showed significantly higher death rates compared with wild-type prawns when challenged with Vibrio parahaemolyticus or Aeromonas hydrophila (Sun et al, 2017). The molt-inhibiting hormone (EcMIH), β, β-carotene 9 , and 10 -oxygenase (EcBCO2) genes, and the carotenoid isomerooxygenase gene EcNinaB-X1 were also successfully knocked out using CRISPR technology.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 System-Mediated Gene Editing in the Fujian Oysters (Crassostrea angulate) by Electroporation

et al. 2021

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The Fujian oyster (Crassostrea angulate) is an important marine bivalve mollusk with high economic value. Gene function research and gene editing techniques have broad application prospects in oyster. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been widely used for genome engineering in many species. CRISPR-mediated gene editing has also been used successfully in the Pacific oyster through direct delivery of the CRISPR/Cas9 components into oyster embryos by microinjection. However, the low throughput and operational difficulties associated with microinjection is one of the factors limiting the widespread application of CRISPR/Cas9 in oysters. In this study, we attempted to deliver the CRISPR/Cas9-system into the embryos of C. angulate by electroporation. An all-in-one CRISPR/Cas9 vector plasmid was used as CRISPR/Cas9 system in this study. Electroporation was carried out using both eggs and blastula larvae. A large number of larvae became malformed or die after electroporation. A single base substitution mutation was detected in the D-larvae developed from electroporated eggs. Our results demonstrate that the CRISPR/Cas9 system can be delivered into embryos of C. angulate for gene editing by electroporation, which provides a reference and will further contribute to the future application of electroporation in mollusks.

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“…Therefore, it is mandatory to have a genomic resource and develop confirmatory experimental research. Kumagai et al (2017), Sun et al (2017), Clark-Hachtel andTomoyasu (2017), andZhang et al (2018a), presented contributions about this new molecular tool. Site directed mutagenesis techniques such as genome editing is an innovative and potential improvement for crustacean or fish aquaculture.…”

Section: Genome Editing In Crustacean Models: Innovative Tool For the Genetic Improvement Of Penaeidsmentioning

confidence: 99%

Research frontiers in penaeid shrimp reproduction: Future trends to improve commercial production

2019

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Shrimp farming worldwide is based on a similar technological package, characterized by three phases: Controlled Reproduction, Larvae Culture, and Grow-out Culture. This basic aquaculture package uses broodstock animals, with different levels of fundamental genetic selection, induced to mature and reproduce based on unilateral eyestalk ablation. This review identified ten reproduction-related research subjects that can improve the shrimp industry based on basic scientific knowledge and four levels of application: eyestalk ablation alternatives, larvae production, product protection, and grow-out yield improvement. Species-specificity must be considered in developing biotechnology solutions. Alternatives to eyestalk ablation for controlling ovarian maturation are based on neurotransmitter regulation in Litopenaeus; however, the environmental impact of this approach has to be evaluated. Maturation by RNAi requires further evaluation, and maturation pheromones have not been explored. Sex reversal, hybridization, in vitro fertilization, and seedstock cryopreservation of penaeids require fundamental research. Triploid culture and genetic selection can be applied to some species for product protection; however, for L. vannamei, triploidization is not practical yet.

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A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda

Cited by 27 publications

References 28 publications

Applying genetic technologies to combat infectious diseases in aquaculture

Applying genetic technologies to combat infectious diseases in aquaculture

CRISPR/Cas9 System-Mediated Gene Editing in the Fujian Oysters (Crassostrea angulate) by Electroporation

Research frontiers in penaeid shrimp reproduction: Future trends to improve commercial production

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