A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection

Recent advances using MXenes in biomedical applications

“…In general, gold nanoparticles are incorporated, especially for the conjugation of DNA probes. [144][145][146][147][148] The sensing range for miRNA, [144][145][146] DNA/RNA, 147,149 or genes 148 is lower than BnM. For the sensing of proteins (e.g., cTnI, 150,151 H5N1 viral proteins, 152 IL-6 153,154 or Sortase A 142 ), detection limits reach as low as Bfg mL À1 .…”

“…In general, gold nanoparticles are incorporated, especially for the conjugation of DNA probes. 144–148 The sensing range for miRNA, 144–146 DNA/RNA, 147,149 or genes 148 is lower than ∼nM. For the sensing of proteins ( e.g.…”

Hymenaea courbaril resin-mediated gold nanoparticles as catalysts in organic dyes degradation and sensors in pharmaceutical pollutants

“…Furthermore, AuNPs is also getting popular due to its simple preparation procedure [ 24 ]. To date, colorimetric assays based on AuNPs have been effectively employed for the detection of a wide spectrum of analytes, encompassing small molecules, nucleic acids, proteins, and metal ions [ [25] , [26] , [27] ].…”

CRISPR/Cas12a-Derived Photoelectrochemical Aptasensor Based on Au Nanoparticle-Attached CdS/UiO-66-NH2 Heterostructures for the Rapid and Sensitive Detection of Ochratoxin A

“…20,21 For instance, Li's team reported a CRISPR/Cas12a-assisted electrochemical aptasensor for nucleic acid analysis based on Au NPs/MXene electrodes. 22 The target nucleic acid was used as an "activator" to specifically bind to programming CRISPR-RNA (crRNA) and trigger the trans-cleavage activity, 23 leading to the cleavage of ssDNA and the changes in sensing interface characteristics that can be employed for target quantification. When the target is absent, the activity of CRISPR/Cas12a is in a "locked" state.…”

“…Considering the high homology and low concentration in real samples, exploring an effective target recognition and amplification strategy to improve the sensitivity and selectivity of aptasensors is essential. Recently, the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas12a detection technology has been applied for aptasesnor construction, owing to its excellent specificity and sensitivity. , Particularly, it possesses trans-cleavage activity and can indiscriminately cleave any ssDNA. , For instance, Li’s team reported a CRISPR/Cas12a-assisted electrochemical aptasensor for nucleic acid analysis based on Au NPs/MXene electrodes . The target nucleic acid was used as an “activator” to specifically bind to programming CRISPR-RNA (crRNA) and trigger the trans-cleavage activity, leading to the cleavage of ssDNA and the changes in sensing interface characteristics that can be employed for target quantification.…”