A Coumarin from Mallotus resinosus that Mediates DNA Cleavage

Abstract

A crude extract prepared from roots of Mallotus resinosus exhibited significant Cu(2+)-dependent DNA strand scission activity and was thus selected for bioassay-guided fractionation. Scopoletin (1), a simple coumarin, was identified as the active principle responsible for DNA cleavage activity of the crude extract. The DNA strand scission activity of 1, as well as that of three structural analogues, is reported.

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

“…The plants of the genus Mallotus are a rich source of biologically active compounds such as phloroglucinols, tannins, terpenoids, coumarins, benzopyrans, and chalcons (Amakura & Yoshida 1996;Tanaka et al 1998;Cheng & Chen 1999;Huang et al 1999;An et al 2001An et al , 2003Chung et al 2002;Van Kiem et al 2004;Supudompol et al 2004;Wei et al 2004;Ma et al 2004;Likhitwitayawuid & Supudompol 2005). Biological activities of the extracts of the plants belonging to the genus Mallotus, or of individual chemical constituents isolated from these extracts, have been reported by several authors.…”

Mallotus species from Vietnamese mountainous areas: phytochemistry and pharmacological activities

“…The existing knowledge about the other investigated plants is in most cases very limited. However, some data underline the isolation of a novel furanocarboxamide from M. cuneatus (Groweiss et al 1994), scopoletin from M. resinosus (Ma et al 2004), phloroglucinol derivatives from M. pallidus (Supudompol et al 2004; or triterpenoids and casbane-type diterpenoid lactones from M. hookerianus (Hui and Li 1976;Bai et al 2006).…”

“…Coumarins, isocoumarins and coumarinolignoids (Table 11) Scopoletin (64), a simple coumarin, was detected in M. resinosus (Ma et al 2004). Isoscopoletin (65) was obtained from the leaves of M. apelta (Kang and Lu 2007).…”

Screening and mechanism study of components targeting DNA from the Chinese herb Lonicera japonica by liquid chromatography/mass spectrometry and fluorescence spectroscopy

“…cisplatin). Considering the various structure types and biological activities of natural products, researchers recently reported the detection and isolation of biological active agents from extracts of natural products, using DNA as an affinity probe, and consequently a range of naturally occurring agents that function by interaction with DNA were found (Correa et al, 2007;Deng et al, 2005;Ma et al, 2004;Pham et al, 2005).…”

“…A conventional procedure for discovery of bioactive compounds bound to DNA from natural products is DNA bioassay-guided detection and isolation (Deng et al, 2005;Ma et al, 2004;Pham et al, 2005), which seems to be time-consuming and laborious. Recently, a simple method of biological fingerprinting chromatogram analysis using high-performance liquid chromatography (HPLC), which is advantageous for eliminating the need of purification of the component, has been successfully devised for the screening bioactive compounds targeted to DNA from CHMs, such as Coptis chinensis, Rheum palmatum and Phellodendron amurense (Su et al, 2005(Su et al, , 2006.…”