A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles

Le, Thao Nguyen; Tran, Tai Duc; Kim, Moon Il

doi:10.3390/nano10010092

Cited by 51 publications

(24 citation statements)

References 35 publications

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“…To the best of our knowledge, chitosan stabilized AgNPs have not been previously used for LPS detection. In a recent publication, chitosan-coated iron oxide magnetic nanoparticles were reported for the colorimetric detection of E. coli and the Gram-positive Staphylococcus aureus [49]. A similar electrostatic interaction was observed showing the universality of the nanoparticle-LPS detection.…”

Section: Discussionmentioning

confidence: 64%

Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria

et al. 2020

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Negatively charged lipopolysaccharide (LPS), a major endotoxin and component of the outer membrane of several Gram-negative bacteria, provides a useful biomarker for the indirect detection of these pathogens. For instance, Escherichia coli (E. coli) is a pathogenic bacterium that causes infections in almost all age groups, and has been implicated in food and water contamination. Current diagnostic and detection methods tend to be labor-intensive or expensive, necessitating the need for an easy, sensitive, rapid, and low-cost method. We report on the synthesis and use of positively charged chitosan stabilized silver nanoparticles (Chi-AgNPs) as a sensitive electrochemical nanobiosensor for the detection of LPS. Chi-AgNPs were synthesized through a facile, single step protocol, and characterized for size, charge, and morphology. Glassy carbon electrodes modified with Chi-AgNPs resulted in an enhancement of signal in the presence of both LPS and E. coli. Detection was accomplished over a large concentration range (several orders of magnitude) of 0.001–100 ng/mL and 10–107 CFU/mL. The biosensors can reliably detect LPS and E. coli at very low concentrations. Chi-AgNPs have potential as low cost, sensitive nanobiosensors for Gram-negative bacteria due to strong electrostatic interaction with LPS present in their outer membranes.

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Section: Discussionmentioning

confidence: 64%

Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria

et al. 2020

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“…As the extinction coefficient of Au nanoparticles is several orders of magnitude larger than those of traditional organic dye molecules, upon formation of Au nanoparticle aggregates, the solution color will change from red to purple for Au nanoparticles, which can be easily visualized by naked eyes. Lei et al reported a simple, reliable, and economical colorimetric assay using the peroxidase-like activity of chitosan-coated iron oxide MNPs [43]. This simple colorimetric strategy allows for the rapid detection of bacterial cells down to 10 4 CFU/mL by the naked eye and 10 2 CFU/mL using spectrophotometry within 10 min.…”

Section: Colorimetric Detectionmentioning

confidence: 99%

Harnessing the Intriguing Properties of Magnetic Nanoparticles to Detect and Treat Bacterial Infections

Xiang

Akakuru

et al. 2021

Magnetochemistry

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Infections caused by pathogenic bacteria, especially multidrug-resistant bacteria, have become a serious worldwide public health problem. Early diagnosis and treatment can effectively prevent the adverse effects of such infections. Therefore, there is an urgent need to develop effective methods for the early detection, prevention, and treatment of diseases that are caused by bacterial infections. So far, magnetic material nanoparticles (MNPs) have been widely used in the detection and treatment of bacterial infections as detection agents and therapeutics. Therefore, this review describes the recent research on MNPs in bacterial detection and treatment. Finally, a brief discussion of challenges and perspectives in this field is provided, which is expected to guide the further development of MNPs for bacterial detection and treatment.

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“…Magnetic nanoparticles (MNPs) have also been explored for the specific characterization of pathogenic bacteria, as they show remarkable enzyme activity with extremely high stability and they can be mass-produced at low cost. Pathogen-specific receptors like antibodies are therefore conjugated on the surface of the nanoparticles and are utilized to identify bacteria (Le, Tran, & Kim, 2020). Shams et al designed an immunosensor for characterization ofBrucella abortus based on the blue-silica nanoparticles (SiNPs) and paramagnetic nanoparticles (PMNPs).…”

Section: Sandwich Immunoassay Based On the Silica-and Magnetic-nanoparticles For Detection Of Brucellamentioning

confidence: 99%

Biosensors functionalized with nanoparticles for rapid detection of Brucella

Ahangari

Mahmoodi

Mohammadzadeh

2021

Preprint

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Brucellosis is an infectious bacterial disease that mainly affects ruminants and humans and its detection is still a significant challenge in particular, in the developing countries. At present, methods used to diagnose brucellosis consist of microbiological, biochemical, immunological, and molecular techniques which may be time-consuming, laborious, expensive, and dependent on special equipment, while they may not be suitable for on-site miniaturized detection. Thus, development of novel methods that can characterize Brucella spp with minimal equipment is invaluable for rapid and easy detection of these pathogenic bacteria especially in basic laboratories or even in the farm fields. The nanotechnology-driven approach can be successfully applied using different nanoparticles for the diagnosis of pathogens. Nanobiosensors are therefore, one of those promising solutions in this case. These sensors are efficient tools with diverse biological applications which can identify pathogenic bacteria with relatively high sensitivity and specificity. In this context, new nanobiosensors, developed based on various nanoparticles, for rapid detection of Brucella spp have been discussed.

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A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles

Cited by 51 publications

References 35 publications

Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria

Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria

Harnessing the Intriguing Properties of Magnetic Nanoparticles to Detect and Treat Bacterial Infections

Biosensors functionalized with nanoparticles for rapid detection of Brucella

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