A conformally adapted all-in-one hydrogel coating: towards robust hemocompatibility and bactericidal activity

Zhang, Fanjun; Hu, Cheng; Yang, Li; Liu, Kunpeng; Yao, Ge; Wei, Yuan; Wang, Jingyu; Luo, Rifang; Wang, Yunbing

doi:10.1039/d1tb00021g

Cited by 42 publications

(29 citation statements)

References 36 publications

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“…After irradiation under UV for 1 h, a thin and robust hydrogel layer was generated on the PVC tube; using the same method, hydrogel coatings were also prepared with PDMS and a polyurethane substrate, as reported in our previous research. 48 The surface-initiated polymerization mechanism is described as follows. Due to the dehydrogenation of benzophenone, active free radicals formed in the PVC polymer backbone could initiate the polymerization of the monomers under UV irradiation.…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 1C, SA (poly(SMBA-co-AAc) hydrogel coating) displayed a wrinkled surface topography under a low magnification, in accordance with the previous report. 48 After peptide grafting, the surface topography changed slightly due to the weak crosslink. The cross-sectional images of the hydrogel coating are shown in Figure 1D and Figure S2; under a high magnification, a thin hydrogel layer containing some micropores was observed.…”

Section: Resultsmentioning

confidence: 99%

“…The amount of AMPs loaded in the hydrogels was determined by the concentration difference before and after AMP grafting, and the concentration of the AMPs was determined by high-performance liquid chromatography as we previously reported. 48 The hydrogel coating grafted with AMPs WR (amino acid sequence: WRWRWR-NH 2 ) and Bac2A (amino acid sequence: RLARIVVIRVAR-NH 2 ) was named as SA-WR and SA-Bac2A, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Dressing Blood-Contacting Materials by a Stable Hydrogel Coating with Embedded Antimicrobial Peptides for Robust Antibacterial and Antithrombus Properties

Liu

Zhang

Wei

et al. 2021

ACS Appl. Mater. Interfaces

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Although dressing blood-contacting devices with robust and synergistic antibacterial and antithrombus properties has been explored for several decades, it still remains a great challenge. In order to endow materials with remarkable antibacterial and antithrombus abilities, a stable and antifouling hydrogel coating was developed via surface-initiated polymerization of sulfobetaine methacrylate and acrylic acid on a polymeric substrate followed by embedding of antimicrobial peptides (AMPs), including WR (sequence: WRWRWR-NH 2 ) or Bac2A (sequence: RLARIVVIRVAR-NH 2 ) AMPs. The chemical composition of the AMP-embedded hydrogel coating was determined through XPS, zeta potential, and SEM−EDS measurements. The AMPembedded antifouling hydrogel coating showed not only good hemocompatibility but also excellent bactericidal and antiadhesion properties against Gram-positive and Gram-negative bacteria. Moreover, the hydrogel coating could protect the AMPs with long-term bioactivity and cover the positive charge of the dotted distributed AMPs, which in turn well retained the hemocompatibility and antifouling capacity of the bulk hydrogels. Furthermore, the microbiological results of animal experiments also verified the anti-infection performance in vivo. Histological and immunological data further indicated that the hydrogel coating had an excellent anti-inflammatory function. Therefore, the present study might provide a promising approach to prevent bacterial infections and thrombosis in clinical applications of blood-contacting devices and related implants.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Dressing Blood-Contacting Materials by a Stable Hydrogel Coating with Embedded Antimicrobial Peptides for Robust Antibacterial and Antithrombus Properties

Liu

Zhang

Wei

et al. 2021

ACS Appl. Mater. Interfaces

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“…S1-S4). The inhibition zone test was employed to analyze the ToAP2D antifungal activities (Zhang et al, 2021). The results showed that three peptides had no inhibition zone at the concentration of 1 mg/ml.…”

Section: Antifungal Activity Serum Stability and Acute Toxicity Assays Of Antimicrobial Peptidesmentioning

confidence: 99%

Antifungal Activity of ToAP2D Peptide Against Sporothrix globosa

Yan

et al. 2021

Front. Bioeng. Biotechnol.

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Improving clinical efficacy and reducing treatment time have been the focus of sporotrichosis therapy. Antimicrobial peptides ToAP2A, ToAP2C, and ToAP2D were synthesized on the basis of ToAP2 (AP02759), a peptide derived from the antimicrobial peptide database by the database filtering technology, and their physicochemical characteristics were analyzed. Compared with template peptide ToAP2, the modified peptides had much shorter length, lower molecular weight but significantly greater stability, which in return resulted in increases in the aliphatic index, hydrophilicity, and protein binding ability. Here, we show that the three derived peptides inhibit the growth of Sporothrix globosa, among which ToAP2D had the strongest anti-fungal activity. ToAP2D showed good serum stability without acute toxicity. The ToAP2D treatment inhibited the growth of S. globosa and enhanced apoptosis, which was evidenced by the upregulation of apoptosis-related protein caspase-3. The scanning electron microscopy analysis revealed deformation and rupture of S. globosa. The levels of mitochondrial membrane potential were decreased and that of the reactive oxygen species (ROS) were increased in S. globosa upon ToAP2D treatment. Moreover, ToAP2D activated metacaspase. In the in vivo study, we further demonstrated that ToAP2D inhibited the S. globosa infection of mice footpads, and its efficiency was nearly comparable to itraconazole. In summary, our results suggest that antimicrobial peptide ToAP2D has the potential for sporotrichosis therapy.

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“…Despite the systemic administration of pharmaceuticals such as antiplatelet agents or anticoagulants, which may reduce the risks of thrombosis and inflammation, therapeutic agents can also increase the risk of bleeding, which can be fatal. [8][9][10][11] To address these challenges, blood-compatible catheters that can prevent thrombosis and inflammation are urgently needed. It is well accepted that the adsorption of plasma proteins such as fibrinogen on a foreign synthetic surface initiates the adhesion/activation of blood cells, including platelets and leukocytes, which are primarily responsible for thrombosis and inflammation, respectively.…”

Section: Introductionmentioning

confidence: 99%

A Uniform and Robust Bioinspired Zwitterion Coating for Use in Blood‐Contacting Catheters with Improved Anti‐Inflammatory and Antithrombotic Properties

Liu

Zhang

Lang

et al. 2021

Macromolecular Bioscience

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Inflammation and thrombosis are two major complications of blood-contacting catheters that are used as extracorporeal circuits for hemodialysis and life-support systems. In clinical applications, complications can lead to increased mortality and morbidity rates. In this work, a biomimetic erythrocyte membrane zwitterion coating based on poly(2-methacryloyloxyethyl phosphorylcholine-co-dopamine methacrylate) (pMPCDA) copolymers is uniformly and robustly modified onto a polyvinyl chloride (PVC) catheter via mussel-inspired surface chemistry. The zwitterionic pMPCDA coating exhibits excellent antifouling activity and resists bacterial adhesion, fibrinogen adsorption, and platelet adhesion/activation. The material also demonstrates great hemocompatibility, cytocompatibility, and anticoagulation properties in vitro. Additionally, this biocompatible pMPCDA coating reduces in vivo foreign-body reactions by mitigating inflammatory response and collagen capsule formation, due to its outstanding ability to resist nonspecific protein adsorption. More importantly, when compared with a bare PVC catheter, the pMPCDA coating exhibits outstanding antithrombotic properties when tested in an ex vivo rabbit perfusion model. Thus, it is envisioned that this biomimetic erythrocyte membrane surface strategy will provide a promising way to mitigate inflammation and thrombosis caused by the use of blood-contacting catheters.

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A conformally adapted all-in-one hydrogel coating: towards robust hemocompatibility and bactericidal activity

Abstract: Hospital-acquired infections and thrombosis caused by bacteria attached to the device surface, or fibrin crosslinking owing to platelet accumulation/activation, are major healthcare challenges that cause morbidity and mortality. To prevent...

Cited by 42 publications

References 36 publications

Dressing Blood-Contacting Materials by a Stable Hydrogel Coating with Embedded Antimicrobial Peptides for Robust Antibacterial and Antithrombus Properties

Dressing Blood-Contacting Materials by a Stable Hydrogel Coating with Embedded Antimicrobial Peptides for Robust Antibacterial and Antithrombus Properties

Antifungal Activity of ToAP2D Peptide Against Sporothrix globosa

A Uniform and Robust Bioinspired Zwitterion Coating for Use in Blood‐Contacting Catheters with Improved Anti‐Inflammatory and Antithrombotic Properties

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