A comprehensive overview on alkaline phosphatase targeting and reporting assays

Shaban, Samy M.; Jo, Sae Byeok; Hafez, Eslam; Cho, Jeong Ho; Kim, Dong Hwan

doi:10.1016/j.ccr.2022.214567

Cited by 43 publications

(25 citation statements)

References 508 publications

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“…ALP has been integrated with ELISA as a reporter enzyme due to its catalytic activity . We modified the conventional ELISA using our multicolor sensor for IL-1β detection as an example, which is a well-known biomarker for the dental inflammation .…”

Section: Resultsmentioning

confidence: 99%

“…ALP has been integrated with ELISA as a reporter enzyme due to its catalytic activity. 35 We modified the conventional ELISA using our multicolor sensor for IL-1β detection as an example, which is a well-known biomarker for the dental inflammation. 64 The standard conventional monochromic ELISA for IL-1β depends on the ability of ALP enzyme to hydrolyze pnitrophenol phosphate (p-NPP) into p-nitrophenol (p-NP) which has just one signaling color (yellow color) under alkaline conditions (Figure 4a).…”

Section: And Nitrobenzene (1 Mm)mentioning

confidence: 99%

“…Numerous analytics have been developed for the ALP and IL-1β detection including surface-enhanced Raman spectroscopy, − fluorometric, − colorimetric, − electrochemical, ,− and electrical sensors. − Among them, the colorimetric sensors have proven to be most powerful and effective analytics for early diagnosis with simple and fast quantitative analytical performance based on a reduced instrumentation complexity, enabling them interfaced with a point-of-care (POC) assay. , The colorimetric signaling mechanism of the ALP assay is based on the chromogenic signal difference between the phosphate and the de-phosphated species . Ascorbic acid phosphate (AAP) has been utilized as a specific substrate to initiate the chromogenic signal in the ALP assay. − However, this is fundamental limitation for accurate diagnosis because AAP is being used as therapeutic elements to enhance the osseointegration of dental implants and improve post-surgical periodontal healing, forming baseline interfering signal of the periodontal analytes and prohibiting the accurate ALP quantification. − Therefore, it is critically required to design a signaling pathway that can produce diverse signal readout based on colorimetric responses for achieving multivariate analysis, high-throughput diagnosis, and versatility in dental applications.…”

Section: Introductionmentioning

confidence: 99%

“…33,34 The colorimetric signaling mechanism of the ALP assay is based on the chromogenic signal difference between the phosphate and the dephosphated species. 35 Ascorbic acid phosphate (AAP) has been utilized as a specific substrate to initiate the chromogenic signal in the ALP assay. 36−38 However, this is fundamental limitation for accurate diagnosis because AAP is being used as therapeutic elements to enhance the osseointegration of dental implants and improve post-surgical periodontal healing, forming baseline interfering signal of the periodontal analytes and prohibiting the accurate ALP quantification.…”

Section: ■ Introductionmentioning

confidence: 99%

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Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

et al. 2023

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Alkaline phosphatase (ALP) and interleukin-1beta (IL-1β) are crucial salivary biomarkers for the diagnosis of periodontal disease that harms the periodontal tissue along with tooth loss. However, there has been no way of sensitive and portable detection of both biomarkers in saliva with multivariate signal readout. In this work, we design the multicolorimetric ALP and IL-1β sensing platform based on geometrical transformation of silver nanoplate transducer. By utilizing enzymatic activity of ALP that dephosphorylates p-aminophenol phosphate (p-APP) to p-aminophenol (p-AP), localized surface plasmon resonance properties of silver nanoplate vary with ALP and show a distinct color change from blue to yellow based on a controlled seed transformation from triangular to hexagonal, rounded pentagonal, and spherical shape. The multicolor sensor shows an ALP detection range of 0–25 U/L with a limit of detection (LOD) of 0.0011 U/L, which is the lowest range of LOD demonstrated to date for state-of-the-art ALP sensor. Furthermore, we integrate the sensor with the conventional ELISA to detect IL-1β for multicolor signaling and it exhibits a linear detection range of 0–250 pg/mL and an LOD of 0.066 pg/mL, which is 2 orders of magnitude lower than the monochromic conventional ELISA (LOD of 3.8 pg/mL). The ALP multicolor sensor shows high selectivity with a recovery of 100.9% in real human saliva proving its reliability and suitability for the readily accessible periodontal diagnosis with multivariate signal readout.

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Section: Resultsmentioning

confidence: 99%

Section: And Nitrobenzene (1 Mm)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

et al. 2023

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“…The selection of the substrate for these analytical applications depends mainly on the type of transducer used. Examples of ALP substrates and ALP bioassays strategies Biosensors 2022, 12, 698 2 of 10 include colorimetric, fluorometric, chemiluminescent, and electrochemical techniques among others [8]. Many of these sensing schemes often involve high economic and time costs, as they include several steps and the use of reactants, which can sometimes be very expensive and must be used in a laboratory by specialized personnel.…”

Section: Introductionmentioning

confidence: 99%

Disposable Electrochemical Biosensor Based on the Inhibition of Alkaline Phosphatase Encapsulated in Acrylamide Hydrogels

et al. 2022

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The present work describes the development of an easy-to-use portable electrochemical biosensor based on alkaline phosphatase (ALP) as a recognition element, which has been immobilized in acrylamide-based hydrogels prepared through a green protocol over disposable screen-printed electrodes. To carry out the electrochemical transduction, an electroinactive substrate (hydroquinone diphosphate) was used in the presence of the enzyme and then it was hydrolyzed to an electroactive species (hydroquinone). The activity of the protein within the matrix was determined voltammetrically. Due to the adhesive properties of the hydrogel, this was easily deposited on the surface of the electrodes, greatly increasing the sensitivity of the biosensor. The device was optimized to allow the determination of phosphate ion, a competitive inhibitor of ALP, in aqueous media. Our study provides a proof-of-concept demonstrating the potential use of the developed biosensor for in situ, real-time measurement of water pollutants that act as ALP inhibitors.

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A Concerted Enzymatic and Bioorthogonal Approach for Extra‐ and Intracellular Activation of Environment‐Sensitive Ruthenium(II)‐Based Imaging Probes and Photosensitizers

et al. 2023

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In this article, we report a novel targeting strategy involving the combination of an enzymeinstructed self-assembly (EISA) moiety and a strained cycloalkyne to generate large accumulation of bioorthogonal sites in cancer cells. These bioorthogonal sites can serve as activation triggers in different regions for transition metal-based probes, which are new ruthenium(II) complexes carrying a tetrazine unit for controllable phosphorescence and singlet oxygen generation. Importantly, the environment-sensitive emission of the complexes can be further enhanced in the hydrophobic regions offered by the large supramolecular assemblies, which is highly advantageous to biological imaging.Additionally, the (photo)cytotoxicity of the large supramolecular assemblies containing the complexes was investigated, and the results illustrate that cellular localization (extracellular and intracellular) imposes a profound impact on the efficiencies of photosensitizers.

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A comprehensive overview on alkaline phosphatase targeting and reporting assays

Cited by 43 publications

References 508 publications

Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

Disposable Electrochemical Biosensor Based on the Inhibition of Alkaline Phosphatase Encapsulated in Acrylamide Hydrogels

A Concerted Enzymatic and Bioorthogonal Approach for Extra‐ and Intracellular Activation of Environment‐Sensitive Ruthenium(II)‐Based Imaging Probes and Photosensitizers

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