A comprehensive genetic study reveals a crucial role of <scp><i>CYP90D2/D2</i></scp> in regulating plant architecture in rice (<i><scp>O</scp>ryza sativa</i>)

Li, Hui; Jiang, Ling; Youn, Ji-Hyun; Sun, Wei; Cheng, Zhijun; Jin, Tianyun; Ma, Xiaoding; Guo, Xiuping; Wang, Jiulin; Zhang, Xin; Wu, Fuqing; Wu, Chuanyin; Kim, Seong‐Ki; Wan, Jianmin

doi:10.1111/nph.12427

Cited by 68 publications

(53 citation statements)

References 61 publications

(108 reference statements)

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“…S6), further suggesting that the smg11 mutation influences BR biosynthesis. Although several rice d2 alleles have been previously reported (Hong et al, 2003; Hong et al, 2005; Li et al, 2013), none of these alleles was reported to increase the panicle branches and grain number per panicle. Consistent with the role of SMG11 in panicle branch and grain number control, expression of SMG11 complemented the panicle branch and grain number phenotypes of smg11, and strong expression of SMG11 decreased panicle branches and grain number per panicle.…”

Section: Discussionmentioning

confidence: 94%

SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice

Fang

Huang

et al. 2016

Rice

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BackgroundGrain size is one of key agronomic traits that determine grain yield in rice. Several regulators of grain size have been identified in rice, but the mechanisms that determine grain size and yield remain largely unknown.ResultsHere we characterize a small grain (smg11) mutant in rice, which exhibits small grains, dense panicles and the increased number of grains per panicle. Cloning and sequence analyses of the SMG11 gene reveal that smg11 is a new allele of DWARF2 (D2), which encodes a cytochrome P450 (CYP90D2) involved in brassinosteroid biosynthetic pathway. Overexpression of D2/SMG11 increases grain size and grain weight of wild-type plants. Overexpression of D2/SMG11 at a suitable level also significantly increases grain yield in rice. Cellular analyses indicate that D2/SMG11 controls grain size by promoting cell expansion. Further results reveal that D2/SMG11 influences expression of several known grain size genes involved in the regulation of cell expansion, revealing a novel link between D2/SMG11 and known grain size genes.Conclusions SMG11 controls grain size by promoting cell expansion in grain hulls. SMG11 regulates cell expansion, at least in part, by influencing expression of several grain size genes involved in the regulation of cell expansion. The smg11 is a new allele of DWARF2/D2. The suitable expression of SMG11 increases grain size, grain weight and grain yield. Our findings reveal the functions of D2/SMG11 in grain size and grain yield, suggesting that the suitable expression of D2/SMG11 is a promising approach to improve grain yield in rice.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0136-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 94%

SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice

Fang

Huang

et al. 2016

Rice

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“…In addition, the full-length cDNA of LOC_Os07g38664 (amplified with primers NcoⅠForward/Reverse) was also cloned into the same vector pCAMBIA1381 to create a cDNA complementary vector p35S::cDNA. For the RNA interference experiment, the construct pCUbi1390-Δ FAD2 (ubiquitin promoter and a FAD2 intron inserted into pCAMBIA1390) was used as RNAi vector (Stoutjesdijk et al 2002;Wu et al 2007;Li et al 2013). A 241-bp specific fragment of the LOC_Os07g38664 gene was amplified with primer pairs RNAi-A Forward/Reverse and RNAi-B Forward/Reverse, respectively, and then cloned into the pCUbi1390-ΔFAD2 vector to form a transformation construct (pUbi-RNAi-LOC_Os07g38664).…”

Section: Vector Construction and Rice Transformationmentioning

confidence: 99%

A detailed analysis of the leaf rolling mutant sll2 reveals complex nature in regulation of bulliform cell development in rice (Oryza sativa L.)

Zhang

Jiang

et al. 2014

Plant Biol J

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Bulliform cells are large, thin-walled and highly vacuolated cells, and play an important role in controlling leaf rolling in response to drought and high temperature. However, the molecular mechanisms regulating bulliform cell development have not been well documented. Here, we report isolation and characterisation of a rice leaf-rolling mutant, named shallot-like 2 (sll2). The sll2 plants exhibit adaxially rolled leaves, starting from the sixth leaf stage, accompanied by increased photosynthesis and reduced plant height and tiller number. Histological analyses showed shrinkage of bulliform cells, resulting in inward-curved leaves. The mutant is recessive and revertible at a rate of 9%. The leaf rolling is caused by a T-DNA insertion. Cloning of the insertion using TAIL-PCR revealed that the T-DNA was inserted in the promoter region of LOC_Os07 g38664. Unexpectedly, the enhanced expression of LOC_Os07 g38664 by the 35S enhancer in the T-DNA is not responsible for the leaf rolling phenotype. Further, the enhancer also exerted a long-distance effect, including up-regulation of several bulliform cell-related genes. sll2 suppressed the outward leaf rolling of oul1 in the sll2oul1 double mutant. We conclude that leaf rolling in sll2 could be a result of the combined effect of multi-genes, implying a complex network in regulation of bulliform cell development.

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“…BR-related mutants usually exhibit short and compact stature with deep green and erect leaves and delayed flowering [37,38]. The rice Osdwarf4-1 mutant exhibits erect leaves and slight dwarfism without compromising grain yield [39]; this phenotype is due to loss of function of a cytochrome P450 involved in BR biosynthesis [40]. A dwarf brassinosteroiddeficient mutant of broad bean (Vicia faba L.) created by γ-ray irradiation was defective in sterol C-24 reduction (the metabolism of the sterol 24-methylenecholesterol to campesterol) [41].…”

Section: Brassinosteroidsmentioning

confidence: 99%

Dwarfing of Breadfruit (Artocarpus altilis) Trees: Opportunities and Challenges

Zhou

Taylor

Underhill

2014

AJEA

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Breadfruit [Artocarpus altilis (Parkinson) Fosberg)] is a traditional staple crop grown for its starchy fruit throughout the tropics. It has long been recognized for its potential to alleviate hunger in the region. However, being a tree of 10 -30m, breadfruit is vulnerable to wind damage. Owing to the continuing trend of global climate change, the success of the species as a sustainable crop for delivering local food security is compromised by the likelihood of more intense tropical windstorms in the island nations. Tree height also forms a major constraint to disease management and fruit harvesting. These imperatives have driven an increasing interest in developing breadfruit varieties with short stature. While a great diversity of breadfruit cultivars with varying nutritional and agronomic characteristics exists, the genetic resource showing dwarfing traits is largely uncharacterised. Historically, there has been no intentional breeding for breadfruit cultivars. The long growth cycle, predominantly vegetative propagation and lack of genome information create challenge for crop improvement through traditional breeding. In this review, we highlight the current knowledge of plant dwarfism and its application in agricultural practices and genetic improvement for dwarf phenotype, and present options and tools for breadfruit dwarfing with special reference to natural genetic variability for dwarfing rootstocks, plant growth regulators, potential of mutagenesis and its combination with the currently Review Article

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A comprehensive genetic study reveals a crucial role of CYP90D2/D2 in regulating plant architecture in rice (Oryza sativa)

Cited by 68 publications

References 61 publications

SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice

SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice

A detailed analysis of the leaf rolling mutant sll2 reveals complex nature in regulation of bulliform cell development in rice (Oryza sativa L.)

Dwarfing of Breadfruit (Artocarpus altilis) Trees: Opportunities and Challenges

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