SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice

Fang, Na; Xu, Ran; Huang, Luojiang; Zhang, Baolan; Duan, Penggen; Li, Na; Luo, Yuqin; Li, Yunhai

doi:10.1186/s12284-016-0136-z

Cited by 96 publications

(86 citation statements)

References 41 publications

(74 reference statements)

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“…The genomic DNAs from Xiushui09 and Changlixian were also resequenced as control. The candidate causal genes were identified as described before (Fang et al, 2016). Six and three candidate causal SNPs for large8-1 and large8-2 were identified, respectively (Tables S1 and S2).…”

Section: Identification Of the Large8 Genementioning

confidence: 99%

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A mitogen‐activated protein kinase phosphatase influences grain size and weight in rice

Wang

et al. 2018

The Plant Journal

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Grain size and weight are directly associated with grain yield in crops. However, the molecular mechanisms that set final grain size and weight remain largely unknown. Here, we characterize two large grain mutants, large grain8-1 (large8-1) and large grain8-2 (large8-2). LARGE8 encodes the mitogen-activated protein kinase phosphatase1 (OsMKP1). Loss of function mutations in OsMKP1 results in large grains, while overexpression of OsMKP1 leads to small grains. OsMKP1 determines grain size by restricting cell proliferation in grain hulls. OsMKP1 directly interacts with and deactivates the mitogen-activated protein kinase 6 (OsMAPK6). Taken together, we identify OsMKP1 as a crucial factor that influences grain size by deactivating OsMAPK6, indicating that the reversible phosphorylation of OsMAPK6 plays important roles in determining grain size in rice.

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Section: Identification Of the Large8 Genementioning

confidence: 99%

“…MutMap approach was used to identifying the candidate genes for the large8-1 and large8-2 mutants (Abe et al, 2012;Fang et al, 2016).…”

Section: Molecular Cloning Of Large8/osmkp1mentioning

confidence: 99%

A mitogen‐activated protein kinase phosphatase influences grain size and weight in rice

Wang

et al. 2018

The Plant Journal

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“…Comparison of the grain size of ah2 mutant with its wild‐type showed that the grain length and width of the ah2 mutant were reduced by 15.2% and 8.4%, respectively, compared with the wild‐type. Several known genes that affect grain size by modulating cell proliferation and expansion have been reported, which include GL7/GW7, BG1 , GS2, GL3 , GS3, GS5, DSG1 , GW2 , and GW8 (Duan et al ., ; Fang et al ., ). The GL7/GW7, GS2, GL3 , DSG1 , GW2 , and GW8 transcript levels were higher in the ah2 mutant than in the wild‐type, consistent with the ah2 phenotype.…”

Section: Discussionmentioning

confidence: 97%

AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice

Ren

Cui

et al. 2019

The Plant Journal

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Summary The palea and lemma (hull) are grass‐specific organs, and determine grain size and quality. In the study, AH2 encodes a MYB domain protein, and functions in the development of hull and grain. Mutation of AH2 produces smaller grains and alters grain quality including decreased amylose content and gel consistency, and increased protein content. Meantime, part of the hull lost the outer silicified cells, and induces a transformation of the outer rough epidermis to inner smooth epidermis cells, and the body of the palea was reduced in the ah2 mutant. We confirmed the function of AH2 by complementation, CRISPR‐Cas9, and cytological and molecular tests. Additionally, AH2, as a repressor, repress transcription of the downstream genes. Our results revealed that AH2 plays an important role in the determination of hull epidermis development, palea identity, and grain size.

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“…Grain size, which is an important agronomic trait in many crops, is determined by grain width, grain length and grain thickness [35]. Although some genes that manipulate seed size have been identified in crop plants [36], the molecular mechanisms that regulate seed size are still poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of full length amino acid sequence conserved domain and similarity, we subdivided the 171 typical members of the MADS gene family into 5 groups (designated MIKC, Mα, Mβ, Mγ, Mδ), according to the previous classification of MADS proteins from Arabidopsis [9]. Of the 63 inferred physic nut JcMADS proteins, thirty-two were assigned to group MIKC (JcMADS03, 04, 05, 07, 12,16,17,19,20,22,24,25,26,27,28,29,31,32,37,38,42,44,46,47,49,50,52,53,54,55,56,63), thirteen to group Mα (JcMADS01, 06, 08, 11,13,14,33,35,39,43,57,58,59), four to group Mβ (JcMADS09, 48, 51, 60), six to group Mγ (JcMADS10, 15,34,36,41,62) and eight to group Mδ (JcMADS02, 18,21,23,30,40,45,…”

Section: Phylogenetic Relationships Of the Jcmads Proteinsmentioning

confidence: 99%

Genome-wide analysis of physic nut MADS-box gene family and functional characterization of the JcMADS05 gene in transgenic rice

Tang

Wang

Bao

et al. 2020

Preprint

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Background: Physic nut (Jatropha curcas), a non-edible oilseed plant, is among the most promising alternative energy sources because of its high seed oil content, rapid growth and extensive adaptability. Proteins encoded by MADS-box genes are important transcription factors involved in the regulation of plant growth, seed development and responses to abiotic stress. However, there has been no in-depth research on the MADS-box genes and their roles in physic nut. Results:In the present study, 63 MADS-box genes (JcMADSs) were identified in the physic nut genome, and classed into five groups (MIKC, Mα, Mβ, Mγ, Mδ) based on phylogenetic comparison with Arabidopsis homologs. Expression profile analysis based on RNA-seq suggested that many JcMADS genes were expressed most strongly in seeds, and seven of them responded in leaves to at least one abiotic stressor (drought and/or salinity) at one or more time points. Transient expression analysis and a transactivation assay indicated that JcMADS05 is a nucleus-localized transcriptional activator.Plants overexpressing JcMADS05 did not show altered plant growth, but the overexpressing plants did exhibit reductions in grain size, grain length, grain width, 1000-seed weight and yield per plant.Further data on the reduced grain size in JcMADS05-overexpressing plants supported the putative role of JcMADS genes in seed development. Conclusions:This study will be useful in understanding the involvement of MADS-box genes in growth and development in addition to their functions in abiotic stress resistance, and will ultimately form the basis for functional characterization and the exploitation of candidate genes for the genetic engineering of physic nut. BackgroundThe regulation of plant growth, development and stress responses is complex and is coordinated by many mechanisms. These mechanisms are under the control of a series of related genes acting through complex regulatory networks. In these processes, transcription factor (such as members of the MYB, HD-Zip, ARF, NAC, MADS-box, and ERF gene families) specifically recognize cis-regulatory elements present in the promoter regions of these genes, and regulate their expression so as to modulate a wide range of biochemical, physiological and developmental processes [1][2][3][4][5][6] (Ferrandiz All Arabidopsis MADS-box protein sequences were used as queries in a BlastP search to identify physic nut proteins. A Hidden Markov Model (HMM) search was also performed against the physic nut protein database using the MADS-domain PF03106. In total, 63 putative MADS proteins were identified in physic nut, with the presence of the MADS-box domain in each of them being confirmed by a SMART website search. These genes were named sequentially from JcMADS01 to JcMADS63 according to their chromosomal locations (Additional File 1). The 63 JcMADS genes ranged in length from 195 (JcMADS35) to 1164 (JcMADS34), thus potentially the proteins encoded would be from 64 to The research was conceived and designed by XB and YT. The experiments were performed ...

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SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice

Cited by 96 publications

References 41 publications

A mitogen‐activated protein kinase phosphatase influences grain size and weight in rice

A mitogen‐activated protein kinase phosphatase influences grain size and weight in rice

AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice

Genome-wide analysis of physic nut MADS-box gene family and functional characterization of the JcMADS05 gene in transgenic rice

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