A detailed analysis of the leaf rolling mutant <i>sll2</i> reveals complex nature in regulation of bulliform cell development in rice (<i>Oryza sativa</i> L.)

Zhang, J. J.; Wu, Shuwen; Jiang, Ling; Wang, J. L.; Zhang, X.; Guo, Xiuping; Wu, Chuanyin; Wan, Jianmin

doi:10.1111/plb.12255

Cited by 53 publications

(43 citation statements)

References 52 publications

(20 reference statements)

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“…Several genetic regulators of leaf rolling have been identified in rice, and some of the recently identified ones include ROLLED LEAF9/SHALLOT-LIKE1 (Zhang et al, 2009), SHALLOT-LIKE2 (Zhang et al, 2015), ROLLED AND ERECT LEAF1 (Chen et al, 2015), ROLLED AND ERECT LEAF2 (Yang et al, 2016), SEMI-ROLLED LEAF1 (Xiang et al, 2012), SEMI-ROLLED LEAF2 (Liu et al, 2016), ROLLING LEAF14 (Fang et al, 2012), and LAT-ERAL ORGAN BOUNDARIES DOMAIN GENE (Li et al, 2016b). Mutants of these genes show either adaxially (inward) or abaxially (outward) rolled-leaf phenotypes, which is usually associated with altered development of bulliform cells.…”

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confidence: 99%

Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development

et al. 2018

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MicroRNAs are 19- to 22-nucleotide small noncoding RNAs that have been implicated in abiotic stress responses. In this study, we found that knockdown of microRNA166, using the Short Tandem Target Mimic (STTM) system, resulted in morphological changes that confer drought resistance in rice (). From a large-scale screen for miRNA knockdown lines in rice, we identified miR166 knockdown lines (STTM166); these plants exhibit a rolled-leaf phenotype, which is normally displayed by rice plants under drought stress. The leaves of STTM166 rice plants had smaller bulliform cells and abnormal sclerenchymatous cells, likely causing the rolled-leaf phenotype. The STTM166 plants had reduced stomatal conductance and showed decreased transpiration rates. The STTM166 lines also exhibited altered stem xylem and decreased hydraulic conductivity, likely due to the reduced diameter of the xylem vessels. Molecular analyses identified rice (), a member of HD-Zip III gene family, as a major target of miR166; moreover, rice plants overexpressing a miR166-resistant form of resembled the STTM166 plants, including leaf rolling and higher drought resistance. The genes downstream of miR166- consisted of polysaccharide synthesis-related genes that may contribute to cell wall formation and vascular development. Our results suggest that drought resistance in rice can be increased by manipulating miRNAs, which leads to developmental changes, such as leaf rolling and reduced diameter of the xylem, that mimic plants' natural responses to water-deficit stress.

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confidence: 99%

Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development

et al. 2018

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“…Bulliform cells are located between the vascular veins of the leaf in rice. Previous studies have demonstrated that changes in the number and area of the bulliform cells result in leaf rolling ( Zhang et al, 2009 , 2015 ; Li et al, 2010 ). Therefore, we performed transverse sectioning so that we could examine the leaves of OE- OsRRK1 and WT plants.…”

Section: Resultsmentioning

confidence: 99%

“…In rice, two types of leaf rolling (adaxial rolling and abaxial rolling) have been related to the abnormal development of bulliform cells. Changes in the number, volume, and localization of bulliform cells can result in lack of osmotic pressure to support the normal form of the blade, and then, the leaf becomes rolled ( Zhang et al, 2015 ). Generally, a leaf displays adaxial rolling when the number and size of bulliform cells are decreased in rice.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of OsRRK1 Changes Leaf Morphology and Defense to Insect in Rice

Zhao

Shangguan

et al. 2017

Front. Plant Sci.

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It has been reported that the receptor-like cytoplasmic kinases (RLCKs) regulate many biological processes in plants, but only a few members have been functionally characterized. Here, we isolated a rice gene encoding AtRRK1 homology protein kinase, OsRRK1, which belongs to the RLCK VI subfamily. OsRRK1 transcript accumulated in many tissues at low to moderate levels and at high levels in leaves. Overexpression of OsRRK1 (OE-OsRRK1) caused adaxial rolling and erect morphology of rice leaves. In the rolled leaves of OE-OsRRK1 plants, both the number and the size of the bulliform cells are decreased compared to the wild-type (WT) plants. Moreover, the height, tiller number, and seed setting rate were reduced in OE-OsRRK1 plants. In addition, the brown planthopper (BPH), a devastating pest of rice, preferred to settle on WT plants than on the OE-OsRRK1 plants in a two-host choice test, indicating that OE-OsRRK1 conferred an antixenosis resistance to BPH. The analysis of transcriptome sequencing demonstrated that several receptor kinases and transcription factors were differentially expressed in OE-OsRRK1 plants and WT plants. These results indicated that OsRRK1 may play multiple roles in the development and defense of rice, which may facilitate the breeding of novel rice varieties.

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“…The shallot-like 2 rolling leaf mutant (sll2) exhibits adaxially rolled leaves accompanied by increased photosynthesis and reduced plant height and tiller number. The shrinkage of bulliform cells is considered responsible for the formation of inward-curved leaves [24]. NRL2, also known as SRL2 and AVB, encodes a novel protein with unknown biochemical function [25].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids

Wang

Shí

Zhang

et al. 2020

IJMS

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The leaf blade is the main photosynthetic organ and its morphology is related to light energy capture and conversion efficiency. We isolated a novel rice Dynamic Narrow-Rolled Leaf 1 (dnrl1) mutant showing reduced width of leaf blades, rolled leaves and lower chlorophyll content. The narrow-rolled leaf phenotype resulted from the reduced number of small longitudinal veins per leaf, smaller size and irregular arrangement of bulliform cells compared with the wild-type. DNRL1 was mapped to chromosome 7 and encoded a putative 3-deoxy-7-phosphoheptulonate synthase (DAHPS) which catalyzes the conversion of phosphoenolpyruvate and D-erythrose 4-phosphate to DAHP and phosphate. Sequence analysis revealed that a single base substitution (T–A) was detected in dnrl1, leading to a single amino acid change (L376H) in the coding protein. The mutation led to a lower expression level of DNRL1 as well as the lower activity of DAHPS in the mutant compared with the wild type. Genetic complementation and over-expression of DNRL1 could rescue the narrow-rolled phenotype. DNRL1 was constitutively expressed in all tested organs and exhibited different expression patterns from other narrow-rolled leaf genes. DNRL1-GFP located to chloroplasts. The lower level of chlorophyll in dnrl1 was associated with the downregulation of the genes responsible for chlorophyll biosynthesis and photosynthesis. Furthermore, dnrl1 showed significantly reduced levels of aromatic amino acids including Trp, Phe and Tyr. We conclude that OsDAHPS, encoded by DNRL1, plays a critical role in leaf morphogenesis by mediating the biosynthesis of amino acids in rice.

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A detailed analysis of the leaf rolling mutant sll2 reveals complex nature in regulation of bulliform cell development in rice (Oryza sativa L.)

Cited by 53 publications

References 52 publications

Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development

Knockdown of Rice MicroRNA166 Confers Drought Resistance by Causing Leaf Rolling and Altering Stem Xylem Development

Overexpression of OsRRK1 Changes Leaf Morphology and Defense to Insect in Rice

Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids

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