A Comparison of the Relative Amounts of Tissue Esterases in Liver and Kidney Homogenates

Abstract

The relative amounts of the ali-esterases in beef and rabbit kidney and liver homogenates have been evaluated quantitatively, after resolution of the esterases by starch-gel electrophoresis. The relative amounts of total ali-esterases in livers and kidneys of a number of animals and fowl have been determined. The data presented and other unpublished data show that the ali-esterase content of tissues increases with age and that there is a sex difference.

Inhibition of beef liver hydrolytic enzymes by organophosphorus pesticides. Quantal inhibition characteristics of several organophosphorus compounds, using o-nitrophenyl butyrate as substrate

“…It has been demonstrated that organophosphorus pesticides and some of their metabolites inhibit a large number of tissue esterases in vitro (Heath, 1961;Lawrence et al, 1960; Ooms and Brebart-Hansen, 1965; Read and McKinley, 1963;van Aspern and Oppenoorth, 1960).…”

“…Carboxylesterases (previously referred to as aliesterases) have been identified in many different tissues from numerous species including blood serum and plasma (Aldridge, 1962;Bernsohn et al, 1961;Lawrence et al, 1960;Main and Braid, 1962;Seume et al, 1960), human and rat brain , human liver (Ecobichon and Kalow, 1961), and housefly homogenates (van Aspern and Oppenoorth, 1960). Much of the work dealing with the inhibition of carboxylesterases by organophosphorus pesticides has been done on crude tissue homogenates (Bergman and Rimon, 1960;Myers et al, 1957;Read and McKinley, 1963), mainly because of the difficulty involved in the isolation and purification of these enzymes (Bergman and Rimon, 1960;Franz and Krisch, 1966).…”

“…Working Enzyme Solution. The crude tissue homogenate was prepared according to the method used by Read and McKinley (1963). One milliliter of this crude material was introduced onto a column (1.5 X 30 cm.)…”