A comparison of the immune response between early exposed and 1 year post exposure to <i>Bacillus anthracis</i> in Indonesia

Redhono, Dhani; Kusumawardani, Arie; Dirgahayu, Paramasari

doi:10.1088/1755-1315/125/1/012012

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…The study used 40 rats that were divided into different groups (as shown in Table 1): 8 rats were normal, 16 rats had inhaled spores at dose 4x10¹ CFU (8 rats received no treatment, 8 rats received EEP 200 mg) and 16 rats had been injected with 0.2 cc spores subcutaneously or approximately 2x10 11…”

Section: Samples Characteristicmentioning

confidence: 99%

“…Cutaneous anthrax appears primary as a papule that progresses to a large vesicle that may develop an eschar if ruptured. Study conducted by Redhono et al (2018), showed that clinical symptoms in the form of eschar are seen in 11.9 percent of patients with cutaneous anthrax. Patients with cutaneous anthrax may have a resolution of illness or develop signs of disseminated disease, but the risk of dissemination and death can be minimized by treating the patient with antibiotics.…”

Section: Effects Of Eep On Mdamentioning

confidence: 99%

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The Comparison of Propolis Effects on Tumor Necrosis Factor Alpha and Malondialdehyde Between Inhalation and Cutaneous Anthrax Animal Models

Redhono¹,

Sanjaya²,

Werdiningsih³

2021

AJID

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Background: Inflammatory response and oxidative stress can be found in anthrax characterized by increased level of serum Tumor Necrosis Factor Alpha (TNF-α) and Malondialdehyde (MDA). The use of antibiotics in anthrax has been known to cause some disturbing side-effects, such as allergic reaction, nausea, vomiting, and antibiotic resistance. Thus, ethanolic extract of propolis (EEP) might be the alternative regimen, due to its anti-inflammatory and antioxidant properties. This study aimed to compare the effects of ethanolic extract of propolis (EEP) on TNF-α and MDA between the inhalation and cutaneous anthrax animal model. Materials and Methods: This was an experimental study with a post-test-only control group design on 40 samples of Rattus norvegicus. Samples were randomized into 5 groups: control, inhalation anthrax model, inhalation anthrax model + EEP, cutaneous anthrax model, and cutaneous anthrax model + EEP. After 14 days, the level of TNF-α and MDA were measured. To compare the data, we used the ANOVA test continued by the post-hoc Turkey test. Results: The results obtained showed that the level of TNF-α and MDA between the inhalation and cutaneous anthrax animal models treated with EEP were statistically different (p < 0.05). The P5 group showed the lowest level of TNF-α (6.822 ± 0.383 pg/ml) and MDA (2.717 ± 0.383 nmol/ml). Conclusion: EEP has a better effect on reducing TNF-α and MDA in cutaneous anthrax animal models compared to the inhalation anthrax animal model.

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Section: Samples Characteristicmentioning

confidence: 99%

Section: Effects Of Eep On Mdamentioning

confidence: 99%

The Comparison of Propolis Effects on Tumor Necrosis Factor Alpha and Malondialdehyde Between Inhalation and Cutaneous Anthrax Animal Models

Redhono¹,

Sanjaya²,

Werdiningsih³

2021

AJID

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“…There was an anthrax outbreak in Pati Regency in 2007 and then in Boyolali Regency in 2009. Anthrax outbreak then appeared in 2011–2015 in Boyolali, Sragen and Pacitan Regencies ( Redhono et al, 2018 ). In 2019–2020, there were similar outbreak in Gunungkidul district, Yogyakarta.…”

Section: Introductionmentioning

confidence: 99%

The effect of Ethanolic extract of Indonesian propolis on endothelial dysfunction and Multi Organ dysfunction syndrome in anthrax animal model

Redhono

Purwanto

Wasita

et al. 2022

Saudi Journal of Biological Sciences

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“…Anthrax cases in Boyolali and Gunungkidul regency attacked not only livestock but also humans. Some people involved in slaughtering or consuming the ill livestock showed the cutaneous-type and gastrointestinal-type anthrax symptoms, which caused death [9]. One solution made in endemic areas was vaccination using the live Sterne spore vaccine (Anthravet, National Center for Veterinary Biologics known as Pusat Veterinaria Farma, Surabaya Indonesia) to all types of susceptible animals, such as cows, buffalos, goats, sheep, horses, and pigs [7].…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of avirulent strains of Bacillus anthracis from environmental samples in Central Java, Indonesia

Apriliana

Wibawa²,

Ruhiat³

et al. 2021

Int J One Health

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Background and Aim: Anthrax is a non-contagious infectious disease caused by Bacillus anthracis. The bacteria form spores that are resistant to extreme conditions and can contaminate the environment for decades. This study aimed to detect and characterize B. anthracis found in endemic areas of anthrax in Yogyakarta and Central Java province, Indonesia. Materials and Methods: Soil samples were collected from Gunungkidul regency, Yogyakarta province (n=315) and Boyolali regency, Central Java province (n=100). Additional soil samples (n=10) and straw samples (n=5) were obtained from Pati regency, Central Java province. The isolation and identification of B. anthracis were performed using conventional methods: Morphology of bacteria colony in solid media, Gram staining, capsule staining, spores staining, and motility test. Isolates were further identified using polymerase chain reaction (PCR) against Ba813, lef (pXO1), and capC (pXO2) gene. An avirulent vaccine strain of B. anthracis (strain 34F2) was used as a control. Results: Only four samples grew on blood agar with a ground-glass appearance, white-gray colony (Gunungkidul and avirulent strain) or yellowish (Boyolali and Pati). All were Gram-positive, presented chains, square-ended rods, spores, and were then identified as B. anthracis. Boyolali, Pati, and avirulent strain isolates had slightly different characteristics, including the growth of non-mucoid in the bicarbonate agar medium, and their uncapsulated form. The PCR showed two Gunungkidul isolates which amplified three genes, including Ba813, lef, and capC. Contrarily, the other isolates did not amplify the capC gene. Conclusion: Gunungkidul isolates were identified as virulent strains of B. anthracis while Boyolali and Pati isolates were proposed as avirulent strains. This is the first report of isolation and identification of avirulent strains of B. anthracis in Central Java, Indonesia.

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A comparison of the immune response between early exposed and 1 year post exposure to Bacillus anthracis in Indonesia

Cited by 3 publications

References 7 publications

The Comparison of Propolis Effects on Tumor Necrosis Factor Alpha and Malondialdehyde Between Inhalation and Cutaneous Anthrax Animal Models

The Comparison of Propolis Effects on Tumor Necrosis Factor Alpha and Malondialdehyde Between Inhalation and Cutaneous Anthrax Animal Models

The effect of Ethanolic extract of Indonesian propolis on endothelial dysfunction and Multi Organ dysfunction syndrome in anthrax animal model

Isolation and identification of avirulent strains of Bacillus anthracis from environmental samples in Central Java, Indonesia

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