A comparison of the effect of fucoidan from alga Fucus vesiculosus and its fractions obtained by anion-exchange chromatography on HeLa G-63, Hep G2, and Chang liver cells

Zhurishkina, Elena V.; Stepanov, S. I.; Shvetsova, Svetlana V.; Kulminskaya, Anna A.; Lapina, I. M.

doi:10.1134/s1990519x17030117

Cited by 11 publications

(12 citation statements)

References 36 publications

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“…In accordance with our results, treatment of Hep G2 cells with 120 µg/mL of the compounds for 24 h did not modify the cell viability and proliferation [49]. The same was found for the two purified fractions after incubation for 48 and 72 h, whereas the crude fucoidan reduced the cell viability [49].…”

Section: Discussionsupporting

confidence: 89%

“…Two studies on hepatocellular carcinoma cell line Hep G2 further underline the importance of concentration and purity of fucoidan regarding cellular effects [37,49]. In the study of Roshan et al (2014), incubation with high concentrations (200–6000 µg/mL) of Sigma fucoidan for 24 h concentration-dependently reduced the cell proliferation and viability (by about 20% at 200 µg/mL) [37].…”

Section: Discussionmentioning

confidence: 99%

“…In the study of Roshan et al (2014), incubation with high concentrations (200–6000 µg/mL) of Sigma fucoidan for 24 h concentration-dependently reduced the cell proliferation and viability (by about 20% at 200 µg/mL) [37]. Zhurishkina et al investigated both crude fucoidan from F. vesiculosus and two purified fractions obtained by ion-exchange chromatography [49]. In accordance with our results, treatment of Hep G2 cells with 120 µg/mL of the compounds for 24 h did not modify the cell viability and proliferation [49].…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the Effects of Fucoidans on the Cell Viability of Tumor and Non-Tumor Cell Lines

et al. 2019

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Fucoidans extracted from brown algae exert manifold biological activities paving the way for the development of numerous applications including treatments outside tumor therapy such as age-related macular degeneration or tissue engineering. In this study, we investigated the antiproliferative effects of fucoidans extracted from six different algae (Fucus vesiculosus, F. serratus, F. distichus subsp. evanescens, Dictyosiphon foeniculaceus, Laminaria digitata, Saccharina latissima) as well as three reference compounds (Sigma fucoidan, heparin, enoxaparin) on tumor (HL-60, Raji, HeLa, OMM-1, A-375, HCT-116, Hep G2) and non-tumor (ARPE-19, HaCaT) cell lines. All fucoidans were extracted according to a standardized procedure and tested in a commercially available MTS assay. Cell viability was measured after 24 h incubation with test compounds (1–100 µg/mL). Apart from few exceptions, fucoidans and heparins did not impair cell viability. In contrast, fucoidans significantly increased cell viability of suspension cell lines, but not of adherent cells. Fucoidans slightly increased viability of tumor cells and had no impact on the viability of non-tumor cells. The cell viability of HeLa and ARPE-19 cells negatively correlated with protein content and total phenolic content (TPC) of fucoidans, respectively. In summary, none of the tested fucoidans turned out to be anti-proliferative, rendering them interesting for future studies and applications.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparison of the Effects of Fucoidans on the Cell Viability of Tumor and Non-Tumor Cell Lines

et al. 2019

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“…Structure-activity relationships of fucoidan are still a controversy where fucoidan is highly heterogeneous in its structure. A large number of studies on this topic suggest that sulfate content and molecular weight of polymer are major factors related to the antioxidant, anticancer, and immunomodulatory properties of fucoidan [24]. Present evidence suggests that fucoidans from S. polycystum could provide health benefits and could be utilized as candidates in cancer chemotherapy.…”

Section: Discussionmentioning

confidence: 98%

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

et al. 2020

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Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 µg mL−1 and 93.62 ± 3.53 µg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.

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“…Moreover, the anti-tumor effects of fucoidans have been well illustrated because of their interaction with various signaling mechanisms [11][12][13][14]. Fucoidans extracted from various sources, such as Fucus vesiculus, Fucus evanescens, Laminaria japonica, and Macrocystis pyrifera have stimulatory effects on dendritic cells (DCs), macrophages, T cell lymphocytes, and other immune cells [15][16][17][18]. A previous study showed that fucoidan from Ecklonia cava (ECF) also induces activation of immune cells [19].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Immune Checkpoint Inhibitor-Mediated Anti-Cancer Immunity by Intranasal Treatment of Ecklonia cava Fucoidan against Metastatic Lung Cancer

Zhang

Hwang

Yadav

et al. 2021

IJMS

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Although fucoidan, a well-studied seaweed-extracted polysaccharide, has shown immune stimulatory effects that elicit anticancer immunity, mucosal adjuvant effects via intranasal administration have not been studied. In this study, the effect of Ecklonia cava-extracted fucoidan (ECF) on the induction of anti-cancer immunity in the lung was examined by intranasal administration. In C57BL/6 and BALB/c mice, intranasal administration of ECF promoted the activation of dendritic cells (DCs), natural killer (NK) cells, and T cells in the mediastinal lymph node (mLN). The ECF-induced NK and T cell activation was mediated by DCs. In addition, intranasal injection with ECF enhanced the anti-PD-L1 antibody-mediated anti-cancer activities against B16 melanoma and CT-26 carcinoma tumor growth in the lungs, which were required cytotoxic T lymphocytes and NK cells. Thus, these data demonstrated that ECF functioned as a mucosal adjuvant that enhanced the immunotherapeutic effect of immune checkpoint inhibitors against metastatic lung cancer.

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A comparison of the effect of fucoidan from alga Fucus vesiculosus and its fractions obtained by anion-exchange chromatography on HeLa G-63, Hep G2, and Chang liver cells

Cited by 11 publications

References 36 publications

Comparison of the Effects of Fucoidans on the Cell Viability of Tumor and Non-Tumor Cell Lines

Comparison of the Effects of Fucoidans on the Cell Viability of Tumor and Non-Tumor Cell Lines

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

Enhancement of Immune Checkpoint Inhibitor-Mediated Anti-Cancer Immunity by Intranasal Treatment of Ecklonia cava Fucoidan against Metastatic Lung Cancer

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