A comparison of tetrazolium reduction and FDA hydrolysis with other measures of microbial activity

Stubberfield, L.; Shaw, Peter J.

doi:10.1016/0167-7012(90)90026-3

Cited by 90 publications

(48 citation statements)

References 6 publications

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“…The FDA method was also shown to correlate well with some of the most accurate measures of microbial biomass such as ATP content and cell density studies (Stubberfield and Shaw, 1990) and radio-labelled thymidine incorporation into microbial DNA (Federle et al,. 1990).…”

Section: Introductionmentioning

confidence: 99%

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Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils

Adam

Duncan

2001

Soil Biology and Biochemistry

903

344

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Section: Introductionmentioning

confidence: 99%

“…This colourless compound is hydrolysed by both free (exoenzymes) and membrane bound enzymes (Stubberfield and Shaw, 1990), releasing a coloured end product, fluorescein. Fig.…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils

Adam

Duncan

2001

Soil Biology and Biochemistry

903

344

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“…A quantificação da atividade das enzimas esterases (EST) foi realizada utilizando 1g de sedimento seguindo a metodologia proposta por Stubberfield & Shaw (1990). O método é baseado na estimativa da fluoresceína produzida na amostra tratada com solução de diacetato de fluoresceína e incubada 24°C por 75 minutos em mesa agitadora.…”

Section: Parâmetros Microbiológicosunclassified

Caracterização da Atividade Bacteriana e Classificação Trófica do Sedimento Superficial do Reservatório de Funil

2011

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RESUMOO reservatório de Funil funciona como receptor do material particulado originado do curso superior do Rio Paraíba do Sul. O material particulado, ao atingir o reservatório, tende a sedimentar. O material particulado sedimentado, rico em nutrientes e material orgânico, abriga uma grande variedade de organismos incluindo as bactérias. Devido à escassez de estudos relacionados à comunidade bacteriana bentônica dos reservatórios o objetivo deste trabalho foi caracterizar os processos bacterianos atuantes através do número de bactérias (autótrofas e heterótrofas), atividades enzimáticas e metabólicas, bem como caracterizar o estado trófico do sedimento superficial do reservatório de Funil a partir da composição biopolimérica (lipídios, proteínas e carboidratos). Foram coletadas amostras de sedimentos superficiais na entrada do reservatório (A), no corpo central (B) e próximo à barragem (C) do reservatório de Funil, no período de baixa pluviosidade (outubro, 2009). Os parâmetros físico-químicos foram obtidos in situ. A análise granulométrica dos sedimentos mostrou a predominância da fração silte-argilosa. Elevados números de bactérias autótrofas foram encontrados no corpo central (1,77x10 6 células.cm -3 ) e próximo à barragem do reservatório de Funil (1,29x10 6 células.cm -3 ). Somente na entrada do reservatório o número de bactérias heterótrofas foi maior que o número de bactérias autótrofas. O número mais provável da atividade respiratória bacteriana (NMP_ARB) mostrou a predominância das bactérias com metabolismo anaeróbio (fermentativas e sulfato-redutoras). A atividade das enzimas esterases (EST) variou entre 1,18-4,79µg fluoresceína.g -1 .h -1 , a qual foi maior na entrada do reservatório. Entretanto, a atividade do sistema transportador de elétrons foi maior na estação próxima a barragem do reservatório (0,004µg O 2 .h -1 .g -1 ). Os sedimentos superficiais foram classificados como um ambiente meso-oligotrófico baseado nas concentrações de biopolímeros (proteínas e carboidratos). As análises microbiológicas mostraram a necessidade da hidrólise da matéria orgânica que atinge o fundo do reservatório, transformando-a em monômeros e oligômeros para posteriormente serem metabolizadas pelas bactérias. Palavras-chave: Atividade metabólica bacteriana; biopolímeros; estado trófico; reservatório de Funil. ABSTRACT CHARACTERIZATION OF BACTERIAL METABOLISM AND CLASSIFICATION OF THE TROPHIC STATE OF THE SUPERFICIAL SEDIMENT IN FUNIL RESERVOIR.The Funil reservoir works as receiver of the particulate matter brought from the upper reaches of the Rio Paraiba do Sul. When the particulate matter arrives in the reservoir, it tends to settle. The settled, particulate matter rich in nutrients and organic matter, act as a shelter for a variety of organisms including bacteria. Therefore, the aims of this work were the characterization of the acting bacterial process through the number of bacteria (autotrophic and heterotrophic), the metabolic and enzymatic activities, and the characterization of the trophic state of the superfic...

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“…Esterase enzyme activity (EST) was determined using the method described by Stubberfield and Shaw (1990). It is based on fluorogenic compounds, which are enzymatically transformed into fluorescent products that can be quantified by the emission with a spectrophotometer by enzymes that hydrolyze many polymeric biomolecules.…”

Section: Esterase Activitymentioning

confidence: 99%

Superficial distribution of aromatic compounds and geomicrobiology of sediments from Suruí Mangrove, Guanabara Bay, RJ, Brazil

Fontana

Silva

Figueiredo

et al. 2010

An. Acad. Bras. Ciênc.

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The distribution of selected aromatic compounds and microbiology were assessed in superficial sediments from Suruí Mangrove, Guanabara Bay. Samples were collected at 23 stations, and particle size, organic matter, aromatic compounds, microbiology activity, biopolymers, and topography were determined. The concentration of aromatic compounds was distributed in patches over the entire mangrove, and their highest total concentration was determinated in the mangrove's central area. Particle size differed from most mangroves in that Suruí Mangrove has chernies on the edges and in front of the mangrove, and sand across the whole surface, which hampers the relationship between particle size and hydrocarbons. An average 10% p/p of organic matter was obtained, and biopolymers presented high concentrations, especially in the central and back areas of the mangrove. The biopolymers were distributed in high concentrations. The presence of fine sediments is an important factor in hydrocarbon accumulation. With high concentration of organic matter and biopolymers, and the topography with chernies and roots protecting the mangrove, calmer areas are created with the deposition of material transported by wave action. Compared to global distributions, concentrations of aromatic compounds in Suruí Mangrove may be classified from moderate to high, showing that the studied area is highly impacted.

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A comparison of tetrazolium reduction and FDA hydrolysis with other measures of microbial activity

Cited by 90 publications

References 6 publications

Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils

Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils

Caracterização da Atividade Bacteriana e Classificação Trófica do Sedimento Superficial do Reservatório de Funil

Superficial distribution of aromatic compounds and geomicrobiology of sediments from Suruí Mangrove, Guanabara Bay, RJ, Brazil

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