A comparison of Sysmex-XN 2000 and Yumizen H2500 automated hematology analyzers

Abstract

A complete blood count is a highly automated laboratory test. The use of highly advanced measurement methods increases the accuracy and sensitivity of the determination of individual hematological parameters, especially in the case of white blood cells differentiation. Therefore, it is necessary to make comparative analyses, which involve performance of the analyzers used in daily work. The aim of the study was to indicate whether the results obtained using two compared analyzers show significant differences. Materials and methods In this study a comparative analysis of 241 peripheral blood samples from adult patients was performed. The complete blood count results were obtained using two automated hematology analyzers: Sysmex XN-2000 and Horiba Yumizen H2500. The Passing-Bablok regression method and Bland- Altman analysis were also used to evaluate the results received for both analyzers. Results Statistically significant differences were found for four hematological parameters: eosinophil count, immature granulocytes, mean corpuscular hemoglobin concentration (MCHC) and platelet distribution width (PDW). The P value for MCHC was 0.01. Sysmex XN-2000 and Horiba Yumizen H2500 also showed disagreement in plate platelet distribution width (PDW) (P ​= ​0.04). For other parameters both analyzers showed good agreement. Conclusion Based on the results of the study, it was shown that there are significant differences in the measurements of hematological parameters between compared analyzers.

Evaluation of the WBC‐differential flagging performances and accuracy of the HORIBA Yumizen H2500 cell counter in oncology patients from a single institution

“…have previously compared the YH2500 to the Sysmex XN-2000 and concerning the WBC-diff, the results showed a significant difference on the eosinophil and immature granulocyte count. 12 They concluded that the different techniques used in the two analyzers may be the source of variability. However, the flagging performance of the two analyzers was not studied.…”

Contagem de Reticulócitos e suas Frações Imaturas como Indicador Precoce de Alteração Medular em Trabalhadores de Postos de Combustíveis

“…The samples were collected as usual and submitted to automated processing in the Sysmex XN-1000 equipment and, after analyzing the results, selected for counting reticulocytes and their IRF in the same equipment, which uses fluorescent dyes as the technology that bind to the RNA of reticulocytes and IRF 16,17 .…”

“…Values between 25,000 and 85,000/mm³ were considered as reference values for the absolute reticulocyte count in people aged over 30 days of life. However, as there are no absolute reference values for the IRF in the literature, these were analyzed only as research parameters 17 .…”

“…Naoum et al 12 and Ruiz et al 11 already suggested that the functionality and integrity of the bone marrow in these individuals could be estimated through the quantification, of the concentration of reticulocytes [13][14][15][16] in peripheral blood. More recently, with the automated counting of reticulocytes 13,16,17 and immature reticulocytes fractions (IRF), these parameters have gained even greater accuracy for the evaluation of erythropoiesis activity [14][15][16] .…”

Overall equipment effectiveness, efficiency and slide review analysis of high-end hematology analyzers

“…The Yumizen H2500 mixes the samples at 360° to ensure their homogeneity. The analyzer capacity is 120 samples per hour and the sample volume needed for analysis is 110 μl [ 9 ].…”