A Comparison of Microbiological and Radioimmunoassay Methods for the Determination of Pantothenic Acid in Foods

Abstract

Seventy‐five foods were analyzed for the vitamin pantothenic acid using a microbiological assay and a new radioimmunoassay (RIA). The food sample extract used in both assays was the result of dialysis after enzyme hydrolysis. In the method used previously, the food‐enzyme mixture had been filtered. A very high correlation (r2=.94) between the results from the RIA and the microbiological assay was found. There was a statistically significant difference between the two assay results for all foods and for the subgroups meats, breads and cereals, and fruits and vegetables at p=.05. At p = .01 breads and cereals and fruits and vegetables did not have significantly different results between the two assay methods. For all foods and all subgroups, the microbiological assay produced a higher mean result than the RIA. The RIA is an acceptable method for assaying pantothenic acid in breads and cereals and fruits and vegetables. Futher study is needed to determine how components of meat interact with one or both assay systems.

Rich biotin content in lignocellulose biomass plays the key role in determining cellulosic glutamic acid accumulation by Corynebacterium glutamicum

“…Riboflavin (vitamin B2) and folic acid (vitamin B9) were assayed based on the cell growth of the riboflavin and folic acid dependent strain Lactobacillus casei ATCC 7469 [ 28 , 63 ]. Niacin (vitamin B3) and pantothenate (vitamin B5) were assayed based on the cell growth of a niacin and pantothenate auxotrophic strain Lactobacillus plantarum ATCC 8014 [ 63 , 64 ]. Pyridoxine (vitamin B6) and cobalamin (vitamin B12) was assayed based on the cell growth of the pyridoxine dependent strain Saccharomyces carlsbergensis ATCC 9080 [ 30 ] and the cobalamin dependent strain Lactobacillus leichmannii ATCC 7830 [ 63 , 65 ].…”

Pantothenic acid

“…Walsh, Wyse and Hansen (1979) omitted the slow and troublesome filtration step, and used dialysis to isolate the liberated pantothenate from the large amount of protein in the food digest.…”

Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization

“…Alternative chemical assay methods have therefore been developed. For example, the use of radioimmunoassay [6,7] and indirect enzyme immunoassay [8][9][10] have been reported. Radioisotopes and scintillation counting are required for the former, and non-commercially available antisera are needed for the latter.…”